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Automatic microscope focusing method

An automatic focus and microscope technology, applied in the field of micro vision, can solve problems such as the limitation of focus accuracy

Inactive Publication Date: 2014-01-22
BEIHANG UNIV
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Problems solved by technology

The passive autofocus system is simple to implement and easy to use. The current passive autofocus system mainly uses motor-driven platform movement and the use of electric zoom lenses, and uses focus evaluation to feedback the movement, so that the observed object is adjusted to the working distance of the microscope. The method is limited by the motor precision, vibration, response speed and hysteresis error during the motor movement process, which causes the limitation of focusing accuracy

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Embodiment Construction

[0012] Embodiments according to the present invention will be described below with reference to the drawings. In the drawings, like reference numerals denote like elements throughout.

[0013] The optical path of the microscope imaging system according to the embodiment of the present invention is described with reference to the accompanying drawings.

[0014] figure 1 A flow chart of an automatic focusing method according to an embodiment of the present invention is shown. In the autofocus process, the liquid lens is used as the zoom element, the focus evaluation operator is used to evaluate the focus state of the microscopic image in real time, and the focus search algorithm is used to servo-control the driving voltage of the liquid lens until the microscopic image has the highest focus evaluation value, and the microscopic image Focusing is complete when the sharpest state is reached.

[0015] figure 2 The optical path diagram of the microscope system according to the ...

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Abstract

The invention provides an automatic microscope focusing method, comprising the steps of utilizing a liquid lens of which the focal length is capable of being controlled by voltage in a microscope optical path to be used as a zooming element, capturing a video stream while performing continuous stepping changing on the driving voltage of the liquid lens, performing focusing evaluation on each captured image, utilizing a focal point searching method to find the liquid lens voltage, positioned at the optimal focusing state, of the microscopic image, and then driving the liquid lens voltage to such a position to finish the automatic focusing. The automatic microscope focusing method fully utilizes the advantages of fast responding speed and small delaying of the liquid lens and solves the problems of slow and inaccurate focusing during the automatic focusing process by using a traditional mobile lifting table.

Description

technical field [0001] The invention relates to the field of microscopic vision in photoelectric detection technology, and more particularly relates to a method for automatic focusing of microscopic vision. Background technique [0002] Autofocus system is a key component of automatic video microscope observation, measurement and depth information reconstruction. Research on microscope autofocus system has always been a hot spot in microscope research, especially for automatic video microscopes with large numerical aperture, high magnification and small depth of field. [0003] Currently proposed autofocus systems mainly include active autofocus systems based on external auxiliary measurement equipment and passive autofocus systems based on image quality evaluation. The active autofocus system based on external auxiliary measurement equipment adjusts the distance between the optical imaging system and the imaged target measured by the external measurement equipment to achiev...

Claims

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Application Information

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IPC IPC(8): G02B21/24G02B21/36G02B3/14
Inventor 屈玉福杨海娟毛建森马诗洁
Owner BEIHANG UNIV
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