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Marine microbial strain YS0810 capable of yielding alkaline catalase

A technology of catalase and marine microorganisms, applied in the field of marine microorganisms

Active Publication Date: 2014-01-22
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on Acinetobacter, especially Acinetobacter from marine sources.

Method used

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  • Marine microbial strain YS0810 capable of yielding alkaline catalase
  • Marine microbial strain YS0810 capable of yielding alkaline catalase
  • Marine microbial strain YS0810 capable of yielding alkaline catalase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] Embodiment 1——The strain identification of marine microbial strain YS0810

[0103] 1) Identification of morphological characteristics and physiological and biochemical characteristics,

[0104] Preparation of crude enzyme solution

[0105] Pick a ring of strain YS0810 from the preserved slant and insert it into a 250ml Erlenmeyer flask containing 50ml of seed medium, activate it at 25°C and 200r / min for 18h, and inoculate it with an inoculum of 10% (V / V) to In a 250ml Erlenmeyer flask with 50ml of fermentation medium, culture at 25°C and 200r / min for 48h, collect the fermentation broth, centrifuge at 10,000r / min for 10min, collect the bacteria, break it intermittently with ultrasonic waves in an ice bath for 10min, and centrifuge at 10,000r / min for 5min. Take the supernatant to detect the enzyme activity. The fermentation medium (wt%): peptone 1%, beef extract 0.3%, NaCl 0.5%, 0.1%KH 2 PO 4 , 0.02%MgSO 4 ·7H 2 O, pH7.0, sterilized by high pressure steam at 121°C f...

Embodiment 2

[0118] Embodiment 2---Fermentation and cultivation of marine microbial strain YS0810

[0119] 1) Initial culture method

[0120]Pick a ring from the preserved slant and put it into a 250ml Erlenmeyer flask containing 50ml of seed medium, activate and culture it at 25°C and 200r / min for 18h, and inoculate it with a 10% (V / V) inoculum to the Put 50ml of fermentation medium in a 250ml Erlenmeyer flask, culture at 25°C and 200r / min for 48h, collect the fermentation broth, centrifuge at 10000r / min for 10min, and take the supernatant to detect the enzyme activity. The seed medium (wt%): 1% peptone, 0.3% beef extract, 0.5% NaCl, adjust the pH to 7.0, and sterilize by high pressure steam at 121°C for 30 minutes; the fermentation medium (wt%): glucose 1% , yeast extract 0.5%, peptone 0.5%, KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O0.02%, pH adjusted to 7.0-7.5, sterilized by high pressure steam at 121°C for 30min. (For the preparation of catalase and the determination of its activity, refer ...

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Abstract

The invention relates to a marine microbial strain YS0810 capable of highly yielding alkaline catalase. The strain is a wild strain capable of highly yielding alkaline catalase, which is screened from a Qingdao sea-area bottom sludge sample, belongs to Acinetobacter sp. Gram-negative bacilli and has no spores, a milky colony of 2-3mm is formed through carrying out plate culture for 24h, has a smooth surface and regular edges, is sticky and is easy to pick up, the strain is brevibacterium, the size of the strain is relatively small, the length is about 1 micron, and the width is about 0.5 micron. The length of the gene sequence of 16S rDNA of the strain YS0810 is 1443bp. The strain has a short fermentation cycle, relatively high enzyme activity and excellent enzyme performance, can highly yield catalase, and can be applied to clinical analysis, food processing, spinning, paper making, medical appliance disinfection, medicaments and the like.

Description

technical field [0001] The invention relates to the field of marine microorganisms, in particular to a marine microorganism strain YS0810 producing alkaline catalase. Background technique [0002] Microbial catalase (EC1.11.1.6), also known as catalase (CAT for short), is ubiquitous in aerobic microorganisms. As an enzyme scavenger, its main function is to catalyze the decomposition of hydrogen peroxide into oxygen and water, and prevent the excessive accumulation of hydroxyl radicals in the body. Based on its high-efficiency catalytic activity, it has been widely used in clinical analysis, food processing, textile, papermaking, disinfection of medical equipment and anti-tumor drug research. In recent years, catalase has attracted much attention due to its application in the textile industry. Most of the application of catalase is carried out under the condition of alkaline high temperature, but the commercial catalase rarely has this characteristic, thus limiting the furt...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/08C12R1/01
Inventor 孙谧郝建华王伟郑鸿飞陈世建
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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