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Method for preparing soluble yeast glucan

A yeast glucan, soluble technology, used in allergic diseases, drug combinations, etc., can solve the problems of pollution, high cost, hidden safety hazards, etc.

Active Publication Date: 2014-01-22
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical modification mainly increases the solubility of yeast dextran by linking hydrophilic groups, such as phosphorylation, carboxymethylation, and sulfation, but such reactions are usually carried out in organic solvent systems and strong acids. , Strong alkali, not only high cost, serious pollution, but also may cause safety hazards; physical methods are mainly ultrasonic depolymerization and oxidative degradation methods, which increase the solubility of yeast dextran by reducing the molecular weight, but ultrasonic depolymerization can only reduce its molecular weight to a limiting constant value, while oxidative degradation introduces impurities such as oxidants into the dextran

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Preparation of Soluble Yeast Glucan by Mechanochemical Method Scheme 1

[0020] Mix yeast glucan powder and grinding balls (steel balls with a diameter of 3 mm) evenly at a mass ratio of 1:20, and use planetary ball milling to perform mechanochemical treatment, with a power of 900W and a treatment time of 30 minutes. Tap water is passed outside the spherical tank Circulating refrigeration; dissolve the treated yeast glucan in water at a mass ratio of 1:25, and centrifuge at 4000rpm for 20 minutes in a centrifuge to obtain a yeast glucan solution, in which 54% of the yeast glucan is dissolved in Water, 46% yeast glucan is insoluble in water.

Embodiment 2

[0021] Example 2 Preparation of Soluble Yeast Glucan by Mechanochemical Method Scheme 2

[0022] Mix the yeast glucan powder and the grinding ball (ceramic ball with a diameter of 6mm) evenly at a mass ratio of 1:12, and use a planetary ball mill for mechanochemical treatment with a power of 900W and a treatment time of 15 minutes, and tap water is passed outside the spherical tank Circulating refrigeration; dissolve the treated yeast glucan in water at a mass ratio of 1:20, centrifuge at 4000rpm for 20min in a centrifuge to obtain a yeast glucan solution, in which 34% of the yeast glucan is dissolved in Water, 66% of the yeast glucan is insoluble in water; after distilling the solution to volume with water, soluble yeast glucan with a concentration of 8.8g / L is obtained.

Embodiment 3

[0023] Example 3 Preparation of Soluble Yeast Glucan by Mechanochemical Method Scheme 3

[0024] Mix yeast glucan powder and grinding balls (ceramic balls with a diameter of 10mm) evenly at a mass ratio of 1:5, and use planetary ball milling for mechanochemical treatment, with a power of 1200W and a treatment time of 5 minutes, and tap water is passed outside the spherical tank Circulating refrigeration; dissolve the treated yeast glucan in water at a mass ratio of 1:10, centrifuge at 4000 rpm for 20 minutes to obtain a yeast glucan solution, in which 29% of the yeast glucan is dissolved in Water, 71% of the yeast glucan is insoluble in water; after distilling the solution to volume with water, soluble yeast glucan with a concentration of 6.0 g / L is obtained.

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Abstract

The invention discloses a method for preparing soluble yeast glucan. The method comprises the following steps: mixing the powdered yeast glucan with grinding balls according to the mass ratio of 1:(5-20), treating by using planetary ball milling for 5-30min at the power of 900-1200W, mixing the prepared yeast glucan with water according to the mass ratio of 1:(10-25) to dissolve the yeast glucan, centrifuging to remove insoluble substances to obtain the yeast glucan solvent. The method has the characteristics of being simple, rapid, efficient and clean, and the prepared yeast glucan has favorable water solubility and excellent immunological competence.

Description

technical field [0001] The invention relates to the technical field of yeast glucan, in particular to a method for improving the solubility of yeast glucan and preparing soluble yeast glucan. Background technique [0002] Yeast β-D-glucan (referred to as yeast β-glucan or yeast glucan) is a good immune response agent (non-specific immune enhancer), which has the functions of enhancing immunity, anti-tumor, anti-oxidation, promoting Wound healing, anti-radiation and other physiological functions, but due to its special chemical structure, it is insoluble in water and only slightly soluble in dimethyl maple, which seriously restricts the industrial application of yeast dextran. [0003] In order to improve the solubility of yeast glucan, scholars at home and abroad have carried out modification and modification research on it, including chemical modification and physical methods. Chemical modification mainly increases the solubility of yeast dextran by linking hydrophilic gro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02A61P37/04
Inventor 史锋石纪奎李永富
Owner JIANGNAN UNIV
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