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Method for producing cellulase through solid state fermentation by adopting rice straws as main raw material

A technology of rice straw and main raw material is applied in the field of producing cellulase by solid-state fermentation with rice straw as the main raw material, which can solve the problems of high price of pure cellulose and high preparation cost of cellulase, and achieves the goal of being favorable for full development and low in price. , The effect of low production cost

Active Publication Date: 2014-01-15
湖南康捷生物科技有限公司
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Problems solved by technology

[0003] However, existing studies on the preparation of cellulase have shown that the cost of cellulase preparation is relatively high. Since cellulase belongs to the class of inducing enzymes, pure cellulose is generally considered to be the best inducer for cellulase synthesis, but pure fiber Cellulase is expensive, and rural rice straw has a wide range of sources. It is an agricultural product waste. It is not only cellulose itself but also a carbon source for microbial growth. This prompts us to explore and study the method of turning waste into treasure and preparing cellulase at low cost. Invention is under such a guiding ideology, after countless experiments to achieve success

Method used

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  • Method for producing cellulase through solid state fermentation by adopting rice straws as main raw material

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Embodiment 1

[0040] Take a slant strain and add 10ml of 0.9% physiological saline, scrape off the spores on the medium with a bamboo stick, which is the strain spore liquid, take 1ml of the strain spore liquid and put it into a 250ml triangle containing 50ml of seed medium , placed in a shaker. Culture conditions: rotation speed 200rpm, temperature 28°C, constant temperature cultivation for 50 hours, which is the seed liquid for fermentation and enzyme production.

[0041] Cut the rice straw into small pieces of about 1 cm, weigh 20 grams and put it into a 500 ml Erlenmeyer flask, add 50 ml of water adjusted to PH=4.8 with acetic acid and sodium hydroxide, sterilize at 121°C for 20 minutes, cool down and set aside.

[0042] Add 5ml of seed culture solution to the sterilized and cooled fermented enzyme medium and fully mix it, and then culture it statically for 9 days at 28°C, then add 200ml of water, stir it fully with a rod, and place the 250ml Erlenmeyer flask at a temperature of Shake ...

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Abstract

The present invention provides a method for producing cellulase through solid state fermentation by adopting rice straws as a main raw material, wherein a strain Trichodema reesei QZ-5 preserved in the China Center for Type Culture Collection on June 5, 2012 and having a preservation number of CCTCC NO: M2012204 is adopted as a fermentation strain, agricultural product waste rice straws are adopted as a main raw material to carry out solid state fermentation to prepare cellulase, the international standard method recommended by the International Union of Pure and Applied Chemistry (IUPAC) is adopted to determine enzyme activity (ie., filter paper enzyme activity FPA determination), activity of cellulase produced by per g of the rice straw is more than 4 IU / g, and the produced cellulase can be widely used in fields of energy sources, textiles, papermaking, feeds, food and the like, and belongs to the technical field of biology.

Description

technical field [0001] The invention provides a method for preparing cellulase by solid-state fermentation using rice straw as the main raw material. The fermentation strain used is a woody strain with the preservation number of CCTCC M 2012204 preserved in the China Typical Culture Collection Center on June 5, 2012. The enzyme belongs to the Trichodema reesei QZ-5 strain, and the invention is characterized by using agricultural product waste-rice straw as the main raw material for solid-state fermentation to prepare cellulase, which is determined by the international standard method recommended by the International Association of Theoretical and Applied Chemistry (IUPAC) Enzyme activity, that is, the enzyme activity of filter paper is determined by FPA, and converted into the activity of cellulase produced per gram of rice straw can reach more than 4IU / g, and the cellulase produced can be widely used in energy, textile, papermaking, feed, food and other fields , belonging to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12N1/14C12R1/885
CPCC12N9/2437
Inventor 李洪兵张峰张明
Owner 湖南康捷生物科技有限公司
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