Stenotrophomonas sp. strain and application thereof in controlling Cyanobacteria blooms

A technology for oligotrophomonas and cyanobacteria blooms, which is applied to oligotrophomonas Lzh-7 and its application field in cyanobacterial bloom control, can solve the problems of limited processing capacity, secondary pollution and the like, and achieves the preparation of The effect of short cycle, good lethal effect and simple preparation method

Inactive Publication Date: 2014-01-15
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the limited processing capacity of physical and chemical methods in the prior art, and the defects of easily causing secondary pollution, the technical problem to be solved in the present invention is to find efficient algae-dissolving bacteria and to separate and enrich the highly efficient algae-dissolving active substances produced by the metabolism of algae-dissolving bacteria , for safe and efficient control of cyanobacterial blooms

Method used

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  • Stenotrophomonas sp. strain and application thereof in controlling Cyanobacteria blooms
  • Stenotrophomonas sp. strain and application thereof in controlling Cyanobacteria blooms
  • Stenotrophomonas sp. strain and application thereof in controlling Cyanobacteria blooms

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Embodiment 1

[0036] The screening of embodiment 1 algae-lytic bacteria

[0037] Add 10mL of natural water samples collected from the Meiliang Bay waters of Taihu Lake to 90mL of algae liquid of Microcystis aeruginosa 9110 in the logarithmic phase, take the yellow algae liquid two days later, apply the gradient dilution method to the beef extract peptone medium agar plate, and cultivate at 28°C After 24 hours, take a plate with a moderate colony density, and select different strains according to the different colony shapes.

[0038] The screened strains were inoculated into 10 mL of beef extract peptone medium, cultured at 28°C and 200 rpm for 24 hours, and 10 mL of cultured bacterial liquid was added to 90 mL of algae liquid of Microcystis aeruginosa in logarithmic phase. In addition, 10 mL of sterilized beef extract peptone medium was also added to 90 mL of algae liquid as a control. All the algae fluids of the experimental group and the control group were cultured in the light incubator...

Embodiment 2

[0042] Identification of embodiment 2 Stenotrophomonas Lzh-7 bacterial strain

[0043] Lzh-7 with strong algicidal effect was identified by morphological observation, staining, physiological and biochemical reactions, and 16srRNA gene sequence analysis. , arranged singly or in pairs, one end of Comamyte, with motility. The optimum growth temperature is 35°C, obligate aerobic bacteria. On the beef extract peptone medium plate, the colonies are medium-sized, round, yellow or brownish yellow and opaque. Oxidase negative, DNase positive, lysine decarboxylase test positive, glucose oxidative decomposition is slow, can quickly oxidatively decompose maltose, can liquefy gelatin, and nitrate produces nitrogen gas negative. After 16srRNA gene sequence analysis and homology comparison, it was known that it had 99% homology with a Stenotrophomonas strain in GenBank, so it was identified as a Stenotrophomonas bacterium and named Stenotrophomonas Lzh-7. The strain has been preserved in...

Embodiment 3

[0044] Embodiment 3 Stenotrophomonas Lzh-7 fermented liquid and the preparation method of ethyl acetate extract thereof

[0045] Stenotrophomonas Lzh-7 was inoculated in sterilized beef extract peptone medium according to 1% inoculum amount, and cultured on a shaker at 200 rpm at 28°C for 48 hours to obtain a fermentation broth containing Stenotrophomonas Lzh-7. Add ethyl acetate into the fermentation broth at a ratio of 1:1, put it into a shaker and vibrate for 24 hours, and separate the upper layer solution, that is, the ethyl acetate extract. The ethyl acetate was evaporated to dryness, dissolved in water, and filtered through a microporous membrane with a pore size of 0.22 μm for further purification of metabolites.

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Abstract

The invention discloses a Stenotrophomonas sp. strain and application thereof in controlling cyanobacterial blooms. A strain of Stenotrophomonas sp. Lzh-7 (CGMCC No.6548) having significant algae-lysing activity is isolated from Taihu water body. The fact is identified that the effective algae-lysing component hydroquinone is isolated and purified from the metabolites of the strain, wherein the median lethal dose (LD50) of hydroquinone to Microcystis aeruginosa 9110 is 3.9 Mug / mL and the median lethal dose (LD50) of hydroquinone to Synechococcus BN60 is 6.7 Mug / mL. The Stenotrophomonas sp. strain can be applied to the research, development and production of novel biological algaecides and eventually applied to the control of lake cyanobacterial blooms.

Description

technical field [0001] The invention relates to the field of environmental microorganisms, in particular to a strain of Stenotrophomonas sp. Lzh-7 with algae-dissolving activity and its application in the control of cyanobacteria blooms. Background technique [0002] In recent years, cyanobacteria blooms caused by lake eutrophication have polluted the lake water system more and more seriously. Taihu Lake, Chaohu Lake, Dianchi Lake and other freshwater waters have been seriously affected. The environmental and economic problems caused by it have attracted people's attention. Therefore, it is very necessary to explore effective ways to control the cyanobacterial biomass and inhibit the occurrence of cyanobacterial blooms. [0003] The control technology of cyanobacteria bloom can be summarized as physical method, chemical method and biological method. Physical methods such as mechanical algae removal, electromagnetic field algae removal, etc. can be used as auxiliary control ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F1/00C02F3/34C12R1/01
Inventor 杨虹李正华潘建良柳向龙
Owner SHANGHAI JIAO TONG UNIV
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