A fractional extraction method of honey pomelo pomelo peel polysaccharide, its product and its application
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A grading method, the technology of pomelo peel polysaccharide, applied in the application, food science, tobacco and other directions, to achieve good results
Active Publication Date: 2016-07-13
CHINA TOBACCO GUANGDONG IND
View PDF1 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
There is no report on the application of HSDE combined with compound enzyme secondary wall breaking technology in the extraction of pomelo peel polysaccharide
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0039] S1. Take pomelo peel, add 1.5% cellulase and 1.5% pectinase according to the weight of pomelo peel, add water at 50°C, the weight ratio of water and pomelo peel is 25:1, high shear Dispersing and emulsifying machine 10000r / min under the treatment of 10min, enzymatic hydrolysis for 1.5 hours, to obtain enzymatic hydrolysis liquid, take a part of the enzymatic hydrolysis liquid, leaching, to obtain a polysaccharide content of 9.49%
[0040] S2. Take the enzymolysis solution obtained from S1, add ethanol so that the concentration of ethanol in the solution is 45~55%, ethanol analysis at a temperature of 25°C-4°C, centrifuge, and centrifuge to obtain a precipitate 1 and a supernatant 1, and the obtained precipitate Object one is that the yield of a crude polysaccharide is 13.12%, the purity of the polysaccharide in the a crude polysaccharide is 42.2%, and the yield of a polysaccharide after purification is 5.6%,
[0041] S3. Add ethanol to the supernatant obtained in step S...
Embodiment 2
[0043] Example 2 Effects of solid-liquid ratio, enzyme amount, enzymolysis time and enzymolysis temperature on extraction.
[0044] 1. Materials
[0045] The honey pomelo was collected from the pomelo garden in Meizhou, and the honey pomelo with the same variety, size, uniform fruit color, basically the same maturity, no mechanical damage, no disease and pest infestation was selected as the test material. After the peel is cut into thin slices, it is dried in a constant temperature drying oven, and it is ground into powder by a pulverizer for later use. Reagents were analytically pure glucose, absolute ethanol, propylene glycol, 5% phenol, concentrated sulfuric acid, deionized water, chloroform, n-butanol, activated carbon, etc. were purchased from Guangzhou Yima Biotechnology Co., Ltd. Pectinase: Tianjin Enzyme Preparation Factory; Cellulase: Shanghai Boao Biotechnology Co., Ltd.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Property
Measurement
Unit
molecular weight
aaaaa
aaaaa
molecular weight
aaaaa
aaaaa
molecular weight
aaaaa
aaaaa
Login to view more
Abstract
The present invention provides a method for extracting honey pomelo pomelo peel polysaccharides and its products and applications thereof. A preparation method for honey pomelo pomelo peel tobacco humectant is provided. The honey pomelo peel is used as a material, and high-speed shear emulsification is combined with a compound enzyme two The polysaccharides from pomelo peel were extracted by first-time wall-breaking method, and the polysaccharides from pomelo peel were prepared by temperature-variable osmotic precipitation with organic solvents. The molecular weight distribution differences of the polysaccharides were studied by gel chromatography. The determination of the molecular weight of polysaccharides of different grades shows that there are significant differences in the molecular weight of polysaccharides graded by different solvents. Among them, the weight average molecular weight of peak ① of a crude polysaccharide is 24100 Da, the weight average molecular weight of peak ① of b crude polysaccharide is 11700 Da, and the weight average molecular weight of peak ① of c crude polysaccharide is 11700 Da. The weight average molecular weight of peak ① is 2130 Da. It shows that the lower the ethanol concentration, the larger the molecular weight of the precipitated polysaccharide. The optimal moisture retention concentration of pomelo polysaccharide is 1.5g / 100Ml. When different solvents are selected, the moisturizing effect of pomelo polysaccharide is different, and the moisture retention of water-soluble pomelo polysaccharide is significantly higher than that of alcohol-soluble pomelo Polysaccharides, in general, are higher than currently used humectants such as propylene glycol.
Description
technical field [0001] The invention relates to the field of polysaccharide extraction, more specifically, to a method for extracting and grading pomelo pomelo peel polysaccharides and the application of the product. Background technique [0002] Pomelo belongs to Rutaceae citrus and is one of the most popular fruits. Guangdong Meixian pomelo is the leading product of the "three high" agricultural strategy in Guangdong Province. At present, Meizhou golden pomelo has been cultivated for nearly a hundred years. The total area of the city is 400,000 mu, and the output is 300,000 tons. Above, the output of pomelo in the province is as high as 400,000 tons, and it is the largest production base of pomelo in the country. In 1995, it was named the "Hometown of Golden Pomelo" by the Organizing Committee of the first batch of 100 Chinese Specialty Hometowns. At present, the city has a total area of 300,000 mu and an output of more than 300,000 tons. It is the largest production ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more