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Detergent compositions comprising metalloproteases

A technology of metalloprotease and composition, which is applied in the direction of detergent composition, detergent compounding agent, enzyme, etc., and can solve problems such as restrictions on the use of metalloprotease

Inactive Publication Date: 2014-01-08
NOVOZYMES AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the use of metalloproteases in washing and cleaning processes and in detergents is limited

Method used

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  • Detergent compositions comprising metalloproteases
  • Detergent compositions comprising metalloproteases
  • Detergent compositions comprising metalloproteases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0260] Gs1 from Geobacillus stearothermophilus and Bca1 from Bacillus thermolyticus purification of

[0261] Expression of Gs1 and Bca1 proteases in Bacillus subtilis.

[0262] The culture broth was centrifuged (20000 xg, 20 minutes) and the supernatant was carefully decanted from the pellet. The supernatant was filtered through a Nalgene 0.2 μm filter unit to remove remaining Bacillus host cells. The 0.2 μm filtrate was transferred to 50 mM H on a G25sephadex column (from GE Healthcare) 3 BO 3 , 5 mM dimethylglutaric acid, 1 mM CaCl 2 , pH7. G25sephadex-transferred enzyme was applied in 50mM H 3 BO 3 , 5 mM dimethylglutaric acid, 1 mM CaCl 2 , Bacitracin agarose column (from Upfront chromatography) equilibrated in pH 7. After washing the column thoroughly with equilibration buffer, thermolysin was washed with 25% (v / v) 2-propanol in 100mM H 3 BO 3 , 10mM MES, 2mM CaCl 2 , 1M NaCl, pH6 elution. Fractions from the column were analyzed for protease activity (Prot...

Embodiment 2

[0264] Purification of Bm1 from Bacillus megaterium and Bce1 and Bce2 from Bacillus cereus

[0265] Expression of Bm1, Bce1 and Bce2 proteases in Bacillus subtilis.

[0266] The culture broth was centrifuged (20000 xg, 20 minutes) and the supernatant was carefully decanted from the pellet. The supernatant was filtered through a Nalgene 0.2 μm filter unit to remove remaining Bacillus host cells. The 0.2 μm filtrate was transferred to 50 mM H on a G25 sephadex column (from GE Healthcare) 3 BO 3 , 5 mM dimethylglutaric acid, 1 mM CaCl 2 , pH7. G25 sephadex-transferred enzyme was applied in 50mM H 3 BO 3 , 5 mM dimethylglutaric acid, 1 mM CaCl 2 , Bacitracin agarose column (from Upfront chromatography) equilibrated in pH 7. After washing the column thoroughly with equilibration buffer, the M4 protease was washed with 25% (v / v) 2-propanol in 100mM H 3 BO 3 , 10mM MES, 2mM CaCl 2 , 1M NaCl, pH6 elution. Fractions from the column were analyzed for protease activity (Pro...

Embodiment 3

[0268] Characterization of Bm1, Bce1 and Bce2 proteases: pH activity and pH stability

[0269] The Protazyme OL characterization assay was used as described in the "Materials and Methods" section to obtain a pH activity profile at 37°C, as well as a pH stability profile at pH optima (residual activity after 2 hours at the indicated pH values). For the pH stability profile, proteases were diluted 8-fold in different characterization assay buffers to achieve the pH of these buffers and incubated at 37°C for 2 hours. After incubation, the pH of the protease incubation was shifted to the pH optimum of the protease by dilution in pH optimum assay buffer and residual activity was determined. The results are shown in the table below. For Table 3, the activities are relative values ​​to the optimum pH of the samples. For Table 4, the activities are relative values ​​relative to samples maintained at stable conditions (5°C, optimum pH).

[0270] Characterization of Gs1 and Bca1 ...

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Abstract

The present invention relates to the use of Thermolysin-Like Metalloproteases in cleaning processes, such as laundry and dish wash, and in particular to the use in low temperature wash and in removal of egg stains. The invention also relates to detergent compositions and cleaning compositions comprising Thermolysin-Like Metalloproteases.

Description

[0001] Sequence listing involved [0002] This application contains the Sequence Listing in computer readable form, which is hereby incorporated by reference. technical field [0003] The present invention relates to cleaning and / or detergent compositions comprising a metalloprotease (E.C3.4.24). The invention further relates to the use of said metalloproteases in cleaning processes, such as dishwashing and laundry. Furthermore, the present invention relates to methods of performing cleaning such as dishwashing and laundry. Background of the invention [0004] The detergent industry has been employing different enzymes in detergent formulations for over 30 years, the most commonly used enzymes include proteases, amylases and lipases, each of which is suitable for removing different types of stains. Detergent compositions typically contain a complex combination of ingredients in addition to enzymes. For example, most cleaning products contain surfactant systems, bleach or ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11D3/386C12N9/54
CPCC11D3/38681C11D3/386C12Y304/24C12N9/52C12N9/54
Inventor E.P.弗里斯P.R.奥斯特加德A.贝尼P.尼尔森
Owner NOVOZYMES AS
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