Method of preparing flaccid anemone saponins W1 and W3 from rhizome of flaccid anemone

Abstract

The invention discloses a method for simultaneously preparing diwusaponin W1 and W3 from diwu medicinal material with high yield of final product and no toxicity, flammable and explosive solvents involved in the preparation process, comprising: 1) adding diwu medicinal material to 2) Add water to the heating vessel, decoct and filter to keep the residue; 3) Repeat step 2) for 1 to 2 times, and combine the filtered decoction; 4) Combine the combined decoction Put on D-101 macroporous resin column, carry out gradient elution with water, 10%, 50% and 95% ethanol solution successively, collect the eluate after 50% ethanol elution treatment, concentrate and dry under reduced pressure; 5) put The obtained total saponin extract of Diguanin was dissolved in water, and then the solution was loaded on a reversed-phase ODS column, and eluted with 30%, 65% and 100% ethanol solutions in sequence, and detected by TLC, and the samples rich in Diguanin were collected Ethanol eluents with corresponding concentrations of W1 and W3 were dried under reduced pressure; 6) Preparative high-performance liquid chromatography was used to separate and obtain pure diguanin W1 and W3.

Description

technical field [0001] The present invention relates to a kind of preparation kusaponin W 1 and W 3 The method, specifically, relates to a higher yield of the final product, which does not involve toxic and flammable and explosive solvents in the preparation process from the medicinal material Diguanin W simultaneously. 1 and W 3 Methods. Background technique [0002] Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation of the joint synovium. Excessive activation, proliferation and infiltration of T lymphocytes during the body's immune response will aggravate joint inflammation and periosteal tissue hyperplasia. , can cause joint swelling and pain, destruction of articular cartilage, narrowing of the joint space, late joint deformity, and eventually varying degrees of disability. According to statistics, RA occurs all over the world, with an average incidence rate of 1%, while the prevalence rate in my country is 0.3% to 0.4%. If not d...

AI Technical Summary

Problems solved by technology

The preparation method reported in the above-mentioned literature has complex procedures, and the final product yield is low due to repeated use of silica gel column chromatography

Simultaneously, because organic solvents such as chloroform (first class solvent), acetonitrile (second class solvent) and ethyl acetate (flammable and explosive solvent) have been used in the preparation process, the toxicity of belonging to class one and second class solvents is big (carcinogenicity is strong ), serious environmental pollution, and the above-mentioned flammable and explosive solvents are not conducive to safety management in the production process, so they are not suitable for industrial production

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