On-line measurement method of biofilm photobioreactor
A technology of photobioreactor and measurement method, applied in the field of high-efficiency biofilm photobioreactor online measurement, can solve the problem of inhibition of hydrogen production metabolic pathway, low biofilm active biomass, increased substrate and metabolite transmission resistance and light Energy attenuation and other issues, to achieve the effect of increasing the hydrogen production rate
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[0059] 1. Strains and culture medium
[0060] The experiment used the Rhodoseudomonas palustris CQK-01 (abbreviated as PSB CQK-01) isolated, identified and domesticated by the Institute of Engineering Thermophysics of Chongqing University. The culture method of PSB CQK-01 mother liquor is: anaerobic 96h under the conditions of temperature, light conditions, and pH of 30℃, 590nm, 4000lx, 7.0. In view of the physiological characteristics of PSB CQK-01 strain, the medium composition and its ratio are as follows: ZnSO 4 ·7H 2 O(0.001g),(NH 4 ) 6 Mo 7 O 24 ·4H 2 O(0.001g), CaCl 2 (0.01g),MgSO 4 ·7H 2 O(0.2g), NaCl(0.2g), KH 2 PO 4 (0.554g), FeSO 4 ·7H 2 O(0.417g), K 2 HPO 4 ·3H 2 O(1.006g), CO(H 2 N) 2 (1.677g), C 5 H 8 NNaO 4 (0.5g), yeast extract (1.0g), C 6 H 12 O 6 ·H 2 O (10g), 1 mL of growth factor solution. The composition and ratio of growth factors are: biotin 1g / L, pyridoxine hydrochloride 1g / L, riboflavin 1g / L, thiamine hydrochloride 1g / L, and niacin 1g / L. The reagents use...
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