Primer and method for detecting molecular marker of rice aroma allele

Abstract

The invention discloses a primer and a detection method which can detect a screened molecular marker aiming at aromatic rice with multiple types of aroma genes and amplify a molecular marker of multiple types of betaine aldehyde dehydrogenase genes 2 (Badh2 / badh2) which are directly related to rice aroma formation. The detection method comprises the following steps: (1) extracting total DNAs of the rice as a template, and adding the primer for performing polymerase chain reaction (PCR) amplification; (2) performing restriction endonuclease Alu I digestion on a PCR product; (3) performing gel electrophoresis detection on an enzyme-digested product. The situation that only a mutation type of the aroma allele can be identified in the process of detecting a molecular marker of aroma genes in the past is broken, plants containing different types of the aroma genes can be selected in an assisted mode, a mutation type of the aroma allele of a parent aromatic rice variety is not required to determined before the operation of performing novel aromatic rice variety breeding on the molecular marker in an assisted mode, and pre-operation steps of molecular marker-assisted breeding are simplified, so that a breeding objective for breeding a novel aromatic rice variety is rapidly and effectively realized.

Description

technical field [0001] The invention relates to the fields of genetics and plant breeding, specifically screening molecular markers of aromatic rice, primers of the molecular markers and a detection method, which are used to assist conventional breeding in rapid, convenient and accurate selection of aromatic rice. Background technique [0002] Basmati rice is favored by consumers because of its pleasant fragrance during cooking. The price of fragrant rice is generally higher than that of non-perfume rice varieties. With the improvement of people's living standards, the current market demand for fragrant rice is increasing. These have greatly promoted people's genetic research on the aroma characteristics of rice, and also accelerated the process of breeding new varieties of aromatic rice. [0003] Although there were differences in the research results on the genetics of rice aroma in the past, most scholars believe that rice aroma is controlled by a recessive major gene (...

AI Technical Summary

Problems solved by technology

So far, 13 allelic types of aroma genes have been found to be caused by the mutation of the betaine aldehyde dehydrogenase 2 gene. Therefore, it is necessary to explore what kind of mutation the betaine aldehyde dehydrogenase 2 gene selected as the parent of the fragrant rice belongs to. The type of workload is still very large

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