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Ziziphora clinopodioides polysaccharide extract and preparation method thereof

A technology for polysaccharide extraction and lip vanilla, applied in the field of plant polysaccharides, can solve the problems of unsuitable large-scale production, high production cost, poor product quality and the like, and achieves the effects of low production process cost, clear color and no impurities, and high yield.

Inactive Publication Date: 2014-01-01
XINJIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention provides a kind of lip vanilla polysaccharide extract and its preparation method, which overcomes the deficiencies of the above-mentioned prior art, and can effectively solve the problem of acid extraction, alkali extraction Method, enzymatic hydrolysis and ultrasonic extraction have the problems of high production cost, poor product quality and unsuitable for large-scale production when extracting lip vanilla polysaccharide.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1, the lip vanilla polysaccharide extract is obtained according to the following preparation method: the first step, after mixing lip vanilla and distilled water at a mass ratio of 1:35 to 1:45, at a temperature of 90°C to 100°C The water was extracted for 4 hours to 5 hours, the mixed solution after water extraction was filtered and the filtrate was collected, and the filtrate was concentrated to a mass of 20 to 30 times that of the lip vanilla to obtain a concentrated solution; in the second step, the concentrated solution and sevage reagent After mixing together according to the volume ratio of 1:1 to 1:1.5, shake at a speed of 130r / min to 140r / min for 25 minutes to 40 minutes for deproteinization treatment. After the shaking is over, put the mixed solution for static layering After standing until the layer interface is clear, discard the upper sevage layer and leave the lower layer of feed liquid layer; the third step is to add alcohol precipitation solvent...

Embodiment 2

[0015] Example 2, the lip vanilla polysaccharide extract is obtained according to the following preparation method: in the first step, the lip vanilla and distilled water are mixed together in a mass ratio of 1:35 or 1:45, and the temperature is 90°C or 100°C Extract the water for 4 hours or 5 hours, filter the mixed solution after water extraction and collect the filtrate, concentrate the filtrate to a mass of 20 times or 30 times the mass of the lip vanilla to obtain a concentrated solution; in the second step, combine the concentrated solution and sevage reagent After mixing together at a volume ratio of 1:1 or 1:1.5, shake at a speed of 130r / min or 140r / min for 25 minutes or 40 minutes for deproteinization treatment. After the shaking is over, put the mixed solution into a static layer After standing until the layer interface is clear, discard the upper sevage layer and leave the lower layer of material liquid layer; the third step is to add alcohol precipitation solvent ...

Embodiment 3

[0016] Embodiment 3, the difference from the above-mentioned embodiment is that firstly the lip vanilla is crushed to a powder that can pass through a 40-mesh sieve to an 80-mesh sieve, and then the powdered lip vanilla and distilled water are mixed together for water extraction.

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Abstract

The invention relates to the technical field of plant polysaccharide, and discloses a Ziziphora clinopodioides polysaccharide extract and a preparation method thereof. The Ziziphora clinopodioides polysaccharide extract is prepared by the following preparation method: 1, mixing Ziziphora clinopodioides with distilled water according to the mass ratio of (1:35) to (1:45), and then carrying out water extraction for 4-5 hours at the temperature of 90-100 DEG C, filtering the mixed solution after water extraction and collecting filtrate, and concentrating the filtrate to be 20-30 folds of mass of the Ziziphora clinopodioides, so as to obtain concentrated solution; 2, mixing the concentrated solution with a sevage reagent according to the volume ratio of (1:1) to (1:1.5). The invention provides a method for extracting the Ziziphora clinopodioides polysaccharide, so that the defect that the Ziziphora clinopodioides polysaccharide and the preparation method thereof are still blank in the field of plant polysaccharide is compensated; a foundation is provided for later analysis of the Ziziphora clinopodioides polysaccharide.

Description

technical field [0001] The invention relates to the technical field of plant polysaccharides, and relates to a lip vanilla polysaccharide extract and a preparation method thereof. Background technique [0002] lip vanilla ( Ziziphora clinopodioides Lam.) is the dry above-ground part of Lamiaceae Xintahua plant, also known as fragrant Xintahua, small-leaf mint, mountain mint, etc., and the Uyghur name is Suze—recorded in "Uyghur Medicine Chronicle" (volume 1) . Lip vanilla is pungent, cool, and slightly bitter, and has the effects of strengthening the heart and diuresis, regulating qi and reducing phlegm, and reducing inflammation and stagnation. Lip vanilla polysaccharide is a plant polysaccharide. At present, plant polysaccharide extraction methods include acid extraction, alkali extraction, enzymatic hydrolysis and ultrasonic extraction. Carry out extraction, find that there is following defect: acid extraction method can cause the breakage of glycosidic bond in lip va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 田树革丁文欢周晓英李洁
Owner XINJIANG MEDICAL UNIV
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