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Preparation method for gram staining solution quality control product

A technique of Gram staining and liquid quality, which is applied in the field of biomedicine, can solve the problems of wasting time, low work efficiency, complicated operation, etc., and achieve the effect of improving economic benefits, saving time, and simple operation

Inactive Publication Date: 2013-12-25
上海兰卫医学检验所股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The four-step Gram staining method works well, but the operation is more complicated. The staff have to do a bacterial smear specimen every time they operate, which is a waste of time and has low work efficiency. There are high requirements for proficiency, but currently there is no pre-made bacterial smear specimen in the market, which can be used directly by the staff at work

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: First, Escherichia coli is used as a gram-negative bacterium, which is installed in the test tube 1, and Staphylococcus aureus is used as a gram-positive bacterium, which is installed in the test tube 2, and both the test tube 1 and the test tube 2 are placed in the refrigerator Then, take sample 1 and sample 2 out of the refrigerator, add 0.6ml physiological saline to sample 1 and sample 2 respectively, dissolve Escherichia coli and Staphylococcus aureus, and prepare Bacterial suspension; take the test tube again, mark it as sample 3, add 2ml of sterile physiological saline to sample 3, further, add 0.02ml of the bacterial suspension of sample 1 and sample 2 to sample 3 solution on a shaker; finally, take 0.2ml of the diluted bacterial suspension from sample 3 and drop it on the glass slide. The dropped slide is left to dry naturally at room temperature, and the dried slide Put the painted side up and pass it back and forth 4 times over the flame of an alcoh...

Embodiment 2

[0042] Embodiment 2: First, Neisseria meningitidis is used as a gram-negative bacterium, which is installed in the test tube 1, and Bacillus anthracis is used as a gram-positive bacterium, which is installed in the test tube 2, and both the test tube 1 and the test tube 2 are frozen in the refrigerator , dry storage; then, take out sample 1 and sample 2 from the refrigerator, add 0.4ml physiological saline to sample 1 and sample 2 respectively, dissolve Neisseria meningitidis and Bacillus anthracis, and prepare a bacterial suspension; Take the test tube again, mark it as sample 3, add 0.8ml of sterile physiological saline to sample 3, further, add 0.15ml of the bacterial suspension of sample 1 and sample 2 to sample 3, put Mix well on the shaker; finally, take 0.15ml of the diluted bacterial suspension from sample 3, drop it on the glass slide, let the dropped glass slide dry naturally at room temperature, and paint the dried glass slide facing Put it on the flame of the alcoh...

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Abstract

The invention relates to the technical field of biomedical treatment and in particular relates to a preparation method for a gram staining solution quality control product. The preparation method comprises the following steps: firstly, putting two standard strains of gram positive bacteria and gram negative bacteria into two test tubes, and freezing and drying the standard strains for storage in a refrigerator; secondly, taking out the two standard strains from the refrigerator, and adding normal saline respectively for dissolving the gram positive bacteria and the gram negative bacteria to prepare a bacterial suspension; thirdly, adding a sterile saline solution into the bacterial suspension; then adding the two standard strains into the bacterial suspension respectively, and uniformly mixing the bacterial suspension on a vibrator; fourthly, taking out the uniformly mixed bacterial suspension, diluting, and dripping on a slide; fifthly, naturally drying the slide at room temperature; sixthly, ensuring that the coating surface of the dried slide faces up, moving the slide back and forth on flame of an alcohol lamp, and fixing a bacterial film. The preparation method has the characteristics that the time is saved, the operation is simple, the work efficiency of workers is improved, the quality of a staining solution can be detected, and the workers can be supervised.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a method for preparing a Gram stain quality control product. Background technique [0002] Gram staining is a very important differential staining method in bacteriology, founded by a Danish physician in 1884. Bacteria are first dyed with basic dye crystals, and after being stained with iodine liquid coal, they are decolorized with alcohol. Under certain conditions, some bacteria will not be decolorized, and some can be decolorized. Therefore, bacteria can be divided into two groups. Major categories: Gram-positive bacteria that are not decolorized, and Gram-negative bacteria that are decolorized. For the convenience of observation, after decolorization, a red dye was used for counterstaining. The positive bacteria were still purple, and the negative bacteria were stained red. [0003] Gram staining generally includes four steps: initial staining, mordant staining, deco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
Inventor 娄峥
Owner 上海兰卫医学检验所股份有限公司
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