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Preparation method of anti-acid staining solution quality control product

An acid-resistant dyeing and liquid quality technology, which is applied in the field of biomedicine, can solve the problems of wasting time, complicated operation, and low work efficiency, and achieve the effects of improving economic benefits, simple operation, and saving time

Inactive Publication Date: 2013-12-18
上海兰卫医学检验所股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The three-step method of acid-fast staining works well, but the operation is more complicated. The staff have to make a bacterial smear specimen every time they operate, which is a waste of time and has low work efficiency. There are high requirements for proficiency, but currently there is no pre-made bacterial smear specimen in the market, which can be used directly by the staff at work

Method used

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Examples

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Effect test

Embodiment 1

[0026] Concrete implementation steps of the present invention are as follows:

[0027] In the first step, H37Ra Mycobacterium tuberculosis was used as an acid-fast stain-positive strain, and the acid-fast stain-negative strain was placed in two test tubes, labeled as sample 1 and sample 2, and stored in a refrigerator to freeze and dry;

[0028] In the second step, take out sample 1 and sample 2 from the refrigerator, wash the H37Ra Mycobacterium tuberculosis of Sample 1 from the culture medium with 1ml of physiological saline, and take 1ml of H37Ra Mycobacterium tuberculosis liquid and add it to a sterile tissue grinder Grinding to make branched H37Ra Mycobacterium tuberculosis; adding 0.5 ml of normal saline to sample 2, dissolving the acid-fast stain-negative strain, and preparing a bacterial suspension;

[0029] The third step is to take the test tube again and mark it as sample 3; first, add 1ml of sterile saline to sample 3; The bacterial suspension of sample 2 was plac...

Embodiment 2

[0034] Concrete implementation steps of the present invention are as follows:

[0035] In the first step, H37Ra Mycobacterium tuberculosis was used as an acid-fast stain-positive strain, and the acid-fast stain-negative strain was placed in two test tubes, labeled as sample 1 and sample 2, and stored in a refrigerator to freeze and dry;

[0036] In the second step, take out sample 1 and sample 2 from the refrigerator, wash the H37Ra Mycobacterium tuberculosis of Sample 1 from the culture medium with 2ml of physiological saline, and take 1ml of H37Ra Mycobacterium tuberculosis liquid and add it to a sterile tissue grinder Grinding; adding 0.4ml of normal saline to sample 2, dissolving the acid-fast stain-negative strain, and preparing a bacterial suspension;

[0037] In the third step, take the test tube again and mark it as sample 3; first, add 2ml of sterile saline to sample 3; The bacterial suspension of sample 2 was placed on a shaker and mixed;

[0038] In the fourth ste...

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Abstract

The invention relates to the field of biomedical technologies, and provides a preparation method of an anti-acid staining solution quality control product. The method comprises the following steps of: respectively putting H37Ra mycobacterium tuberculosis serving as a positive anti-acid staining strain together with a negative anti-acid staining strain into two test tubes and marking as a sample 1 and a sample 2; putting the samples 1 and 2 into a refrigerator; cleaning the H37Ra mycobacterium tuberculosis of the sample 1 from a culture medium with physiological saline; adding an H37Ra mycobacterium tuberculosis solution into a sterile tissue grinder to be ground so as to prepare branched H37Ra mycobacterium tuberculosis; adding the physiological saline into the sample 2 to dissolve the positive anti-acid staining strain so as to prepare a bacterium suspension; adding the sterile physiological saline into a sample 3; respectively adding the branched H37Ra mycobacterium tuberculosis and the suspension of the sample 2 into the sample 3 and evenly mixing in an oscillator; dripping the bacterium suspension of the sample 3 on a glass slide; performing air drying on the glass slide at a room temperature; putting a coating surface of the dried glass slide upwards; enabling the coating surface of the dried glass slide to pass through the flame of an alcohol lamp back and forth; and fixing a mycoderm. According to the method, the working efficiency of workers is improved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for preparing an acid-fast staining liquid quality control product. Background technique [0002] Acid-fast staining is a bacterial staining method pioneered by Ehrlich in 1882 and improved by Zier. The cell wall of mycobacteria contains a large amount of lipids, which are surrounded by peptidoglycan, so mycobacteria are generally not easy to color, and it is necessary to heat and prolong the coloring time to promote its coloring. However, after the mycolic acid in mycobacteria is combined with the dye, it is difficult to be decolorized by the acid decolorizing agent, so it is called acid-fast staining. Zie-Nissl's acid-fast staining method is to make mycolic acid and phenol fuchsin firmly combine to form a complex under heating conditions, and it will not be decolorized when treated with hydrochloric acid alcohol. After counterstaining with alkalin...

Claims

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Application Information

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IPC IPC(8): C12Q1/00C12R1/32
Inventor 娄峥
Owner 上海兰卫医学检验所股份有限公司
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