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Method for extracting anthocyanin from Europe vaccinium myrtillus

A technology for crude extraction of anthocyanins, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of waste of anthocyanins, low yield, etc., and achieve low reagent consumption, yield and purity High, short-time effect

Active Publication Date: 2013-12-25
三原金瑞生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method only utilizes the juice of lingonberry, and the anthocyanins in the pericarp and pulp are wasted, and the yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Extract anthocyanins from bilberry, the steps are as follows:

[0024] 1) Using bilberry as a raw material, reflux extraction with 50% ethanol solution, adding hydrochloric acid to the ethanol solution, concentrating the extract under reduced pressure to twice the weight of the raw material, passing through a chromatographic column filled with D101 macroporous resin, The flow rate of the upper column is 1BV / h. First, the macroporous resin is washed with water until the effluent is clear. The flow rate of water washing is 0.8BV / h, and then eluted with 50% ethanol solution. The eluate is collected, concentrated under reduced pressure, and dried to obtain a crude extract. thing;

[0025] Wherein, the ethanol solution addition amount during reflux extraction is 9 times of raw material weight, the number of times of reflux extraction is 2 times, each 2h; The mass concentration of hydrochloric acid is 37%, and the addition amount of hydrochloric acid is 0.8% of ethanol soluti...

Embodiment 2

[0028] Extract anthocyanins from bilberry, the steps are as follows:

[0029] 1) Using lingonberry as raw material, reflux extraction with 40% ethanol solution, adding hydrochloric acid to the ethanol solution, concentrating the extract under reduced pressure to twice the weight of the raw material, passing through a chromatography column filled with D101 macroporous resin, The flow rate of the upper column is 1.2BV / h. First, the macroporous resin is washed with water until the effluent is clear. The flow rate of water washing is 0.5BV / h, and then eluted with 40% ethanol solution. The eluate is collected, concentrated under reduced pressure, and dried to obtain crude Extract;

[0030] Wherein, the ethanol solution addition amount during reflux extraction is 9 times of raw material weight, and the number of times of reflux extraction is 3 times, each 1h; The mass concentration of hydrochloric acid is 40%, and the addition amount of hydrochloric acid is 1% of ethanol solution we...

Embodiment 3

[0033] Extract anthocyanins from bilberry, the steps are as follows:

[0034] 1) Using bilberry as a raw material, reflux extraction with 50% ethanol solution, adding hydrochloric acid to the ethanol solution, concentrating the extract under reduced pressure to 2.5 times the weight of the raw material, passing through a chromatographic column filled with D101 macroporous resin, The flow rate of the upper column is 0.8BV / h. First, the macroporous resin is washed with water until the effluent is clear. The flow rate of water washing is 0.7BV / h, and then eluted with 50% ethanol solution. The eluate is collected, concentrated under reduced pressure, and dried to obtain crude Extract;

[0035] Wherein, the ethanol solution addition amount during reflux extraction is 10 times of raw material weight, and the frequency of reflux extraction is 1 time, each 2h; The mass concentration of hydrochloric acid is 50%, and the addition amount of hydrochloric acid is 0.5% of ethanol solution we...

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PUM

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Abstract

The invention relates to a method for extracting anthocyanin from Europe vaccinium myrtillus. The method includes the following steps that 1) the Europe vaccinium myrtillus is used as a raw material, reflux extraction is conduct in a 40%-60% ethanol solution, hydrochloric acid is added into the ethanol solution, after an extracting solution is concentrated, a chromatographic column filled with macroporous resin is used, the macroporous resin is washed until effluent is clarified, then a 40%-60% ethanol solution is used for conducting elution, the eluant is collected, concentrated and dried, and then a crude extract is obtained; 2) subcritical extraction is conducted on the crude extract by using acetone as an extraction agent, the injection rate of the extraction agent is one to two times of the weight of the crude extract, the extraction temperature is 58-62 DEG C, the extraction pressure is 2.8-3.2MPa, the extraction time is 20-40min, and after self-volatilized acetone is recycled, a finished product can be obtained. According to the method for extracting the anthocyanin from the Europe vaccinium myrtillus, the processes of ethanol solution reflux extraction, macroporous resin absorption, subcritical extraction and the like are adopted, the steps are simple, the used time is short, the dosage of reagents is small, the yield is about 0.5%, the content of the anthocyanin is about 70%, the yield and the purity are high, and therefore the method is more suitable for industrial production.

Description

technical field [0001] The invention relates to a method for preparing plant extracts, in particular to a method for extracting anthocyanins from bilberry. Background technique [0002] European lingonberry (Vaccinium myrtillus), also known as black fruit lingonberry, is the fruit of the plant Vaccinium myrtillus in the family Rhododendronaceae. European bilberry contains a large amount of flavonoids, mainly anthocyanins, including cyanidin, delphinidin, paeoniflorin, petunia, cotton sunflower, etc., as well as galactose, Arabinose and glucosides. [0003] Anthocyanin (Anthocyanin), or anthocyanin, anthocyanin, is a common water-soluble plant pigment, widely present in the cell fluid of flowers, fruits, stems, leaves and root organs of plants, and its structure is anthocyanin Glucose and carbohydrates are combined by glycosidic bonds. Due to its unique functionality, it has been used to scavenge free radicals in the body, anti-tumor, anti-cancer, anti-inflammatory, inhibi...

Claims

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Application Information

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IPC IPC(8): C07H17/065C07H1/08C09B61/00
Inventor 文继承蒋元勇朱昂辉雍小军易华李丽金
Owner 三原金瑞生物工程有限公司
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