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Method for measuring total number of fermented milk living bacteria

A technology of fermented milk and live bacteria, which is applied in the direction of biochemical equipment and methods, and the measurement/inspection of microorganisms, which can solve the problems of inappropriate total number of viable bacteria, death of bacteria, and influence of osmotic pressure balance of bacteria, etc.

Active Publication Date: 2013-12-18
湖南南山牧业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the literature, when EDTA solution and milk sample are used to measure the OD value at a ratio of 9:1, it has a great impact on maintaining the osmotic pressure balance of the bacteria, which will cause the death of some bacteria, and is not suitable for the determination of the total number of viable bacteria.

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  • Method for measuring total number of fermented milk living bacteria
  • Method for measuring total number of fermented milk living bacteria
  • Method for measuring total number of fermented milk living bacteria

Examples

Experimental program
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Embodiment 1

[0050] 1. Preparation of test samples: Inoculate Lactobacillus bulgaricus and Streptococcus thermophilus into sterilized milk with a solid mass fraction of skim milk of 11.5% (sterilization temperature is 110°C and time is 15 minutes), among which, The inoculum volume ratio of the strains was 1:1, the total inoculation amount was 5%, and the fermented milk samples were obtained after 9 hours of fermentation at 42°C.

[0051] 2. The configuration of EDTA solution with a solid-liquid ratio of 2g / L: Accurately weigh about 2g of EDTA, in order to fully dissolve EDTA, add 70mL of 1mol / L NAOH solution and distilled water, so that the amount of liquid added is 1L, and the EDTA solution is obtained. The pH value is 13.2.

[0052] 3. Add 15mL of the above sterilized EDTA solution and 25mL of fermented milk to be tested into a clean Erlenmeyer flask on an ultra-clean workbench, and quickly add sterilized normal saline to a total volume of 245mL to obtain a fermented milk dilution. Use ...

Embodiment 2

[0076] 1. Preparation of test samples: Inoculate Lactobacillus casei and Bifidobacterium into sterilized milk with a solid mass fraction of skim milk of 11.5% (sterilization temperature is 110°C and time is 15 minutes). The inoculum amount of the above-mentioned composite strains was 5%, the inoculum volume ratio of Lactobacillus casei and Bifidobacterium was 3:1, the fermentation temperature was 34°C, and the fermentation time was 48 hours to obtain the required samples.

[0077] 2. The configuration of EDTA solution with a solid-liquid ratio of 2g / L: Accurately weigh about 2g of EDTA, in order to fully dissolve EDTA, add 65mL of 1mol / L NAOH solution and distilled water, so that the amount of liquid added is 1L, and the EDTA solution is obtained. The pH is 12.6.

[0078] 3. Add 5mL of the above sterilized EDTA solution and 25mL of fermented milk to be tested into a clean Erlenmeyer flask on an ultra-clean workbench, and quickly add sterilized normal saline to a total volume o...

Embodiment 3

[0101] 1. Preparation of test samples: Inoculate Lactobacillus casei and Bifidobacterium into sterilized milk with a solid mass fraction of skim milk of 11.5% (sterilization temperature is 110°C and time is 15 minutes). The inoculum amount of the above-mentioned composite strains was 5%, the inoculum volume ratio of Lactobacillus casei and Bifidobacterium was 3:1, the fermentation temperature was 34°C, and the fermentation time was 48 hours to obtain the required samples.

[0102] 2. The configuration of the EDTA solution with a solid-liquid ratio of 2g / L: Accurately weigh about 2g of EDTA to fully dissolve the EDTA, add 60.5mL of 1mol / L NAOH solution and distilled water, so that the amount of liquid added is 1L, and the EDTA solution is obtained. The pH is 11.8.

[0103] 3. On the ultra-clean workbench, add 10mL of the above sterilized EDTA solution and 25mL of fermented milk to be tested into a clean Erlenmeyer flask, and quickly add sterilized normal saline to a total volum...

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Abstract

The invention provides a method for measuring the total number of fermented milk living bacteria. The method comprises the steps of adding 2g / L of ethylene diamine tetraacetic acid (EDTA) solution with the pre-dilution volume fraction of 2-6% into a fermented milk sample; diluting the fermented milk sample, and adjusting the pH value to be 6.8-8.8; and carrying out colony counting, and calculating the total number of the fermented milk living bacteria. The method enables fermented milk diluent to become clear within a short time and bacterial cells to be evenly dispersed, and the step of oscillating the sample diluent for about 20 minutes is omitted in a measuring process; the accuracy of a measurement result of the method is higher than that of the measurement result of GB4789.35-2010.

Description

technical field [0001] The invention relates to a food testing method, in particular to a method for measuring the total number of live bacteria in fermented milk. Background technique [0002] Probiotics have a variety of functions to regulate physiological functions, such as promoting digestion, regulating the balance of gastrointestinal flora, correcting intestinal dysfunction, regulating immunity, and lowering serum cholesterol. The main product carrier for probiotics to enter the human body and exert the above effects is probiotic fermented milk. Therefore, the number of live bacteria in probiotic fermented milk is a key factor to ensure its functional properties. To obtain the desired effect, the number of probiotics must reach a sufficient number. An important indicator for judging the quality of commercially available products and fermented milk products prepared by literature methods is to determine the total number of viable bacteria in them. [0003] At present,...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
Inventor 刘成国易文芝周辉
Owner 湖南南山牧业有限公司
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