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Application of metformin on preparation drugs for reducing surface antigen level of hepatitis B virus

A technology of hepatitis B virus and metformin, which is applied in the field of medicine and can solve problems such as Met’s anti-HBV and interfering viral protein reports.

Inactive Publication Date: 2013-12-18
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there have been no reports about Met's anti-HBV and interfering with viral proteins

Method used

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  • Application of metformin on preparation drugs for reducing surface antigen level of hepatitis B virus
  • Application of metformin on preparation drugs for reducing surface antigen level of hepatitis B virus
  • Application of metformin on preparation drugs for reducing surface antigen level of hepatitis B virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 2

[0018] Example 1 Metformin reduces the level of hepatitis B virus surface antigen effect test

[0019] (1) Cell culture, drug intervention and index detection scheme

[0020] Stable cell line HepG 2.2.15 cells were treated with DMEM medium (Gibco company, plus 10% fetal bovine serum, 100 U / ml penicillin, 100 μg / ml streptomycin and 400 μg / ml G418) in 5% CO 2 Culture at a constant temperature of 37°C in a saturated water vapor environment.

[0021] Cells in the logarithmic growth phase are divided into 2*10 4 Spread 24-well plates at a density of 24 wells. After the cells are completely adhered to the wall the next day, set the dosage according to the usual dosage of the in vitro experimental protocol in the reference literature: 5 concentration gradients of 1, 0.5, 0.25, 0.125, and 0.063 (mM) for metformin, The normal control was only given blank medium; each group had three replicate wells; the administration was repeated every other day, and the supernatant was collected 5 ...

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Abstract

The invention belongs to the field of medicine, and relates to a pharmaceutical application of an oral hypoglycemic drug metformin, and especially relates to an application of metformin on preparation drugs for reducing surface antigen level of hepatitis B virus. The results of in-vitro cell culture tests and drug intervention tests show that metformin can prominently reduce HBsAg titer of stable cell line HepG 2.2.15 and HBeAg titer of cell culture supernatant, and has an inhibition tendency on HBV-DNA of HepG 2.2.15 cell culture supernatant and HBV replication intermediates in cells. The metformin can be used to prepare drugs for inhibiting HBsAg, and furthermore, can be used to prepare drugs for reducing or eliminating serum surface antigen of hepatitis B virus chronic infection patients, drugs for reducing or inhibiting core antigen of hepatitis B virus, and drugs for inhibiting DNA and virus replication intermediates of hepatitis B virus.

Description

technical field [0001] The invention belongs to the field of medicine and relates to a new medicinal use of oral hypoglycemic drug metformin. It specifically relates to the use of metformin in the preparation of medicines for reducing the surface antigen level of hepatitis B virus. Background technique [0002] At present, the quantitative monitoring and regulation of hepatitis B virus (HBV) surface antigen (HBsAg) is a hot issue in the field of HBV research. Studies have shown that after antiviral treatment improves the clinical outcome of patients with current HBV infection to a certain extent, the decline of HBV-related hepatocellular carcinoma (HCC) is not ideal, and interferon only marginally reduces HCC (P=0.056), while Medium and long-term treatment of nucleoside (acid) analogues can only partially reduce the risk of HCC but cannot completely eliminate its risk. Once drug resistance is obtained, the risk of HCC cannot be reduced even if the salvage treatment obtains ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/155A61P1/16A61P31/20
Inventor 张继明臧国庆荀运浩
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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