Application of yeast-derived dextran in the preparation of medicines for treating hepatitis B virus infection

A technology of hepatitis B virus and hepatitis B virus, applied in antiviral agents, drug combinations, pharmaceutical formulas, etc., can solve problems such as strong side effects, heavy economic burdens for patients and their families

Inactive Publication Date: 2016-01-20
SHANGHAI PUBLIC HEALTH CLINICAL CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, long-term interferon therapy has strong side effects and brings a heavy economic burden to patients and their families, while nucleoside analog therapy is prone to drug resistance of the virus

Method used

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  • Application of yeast-derived dextran in the preparation of medicines for treating hepatitis B virus infection
  • Application of yeast-derived dextran in the preparation of medicines for treating hepatitis B virus infection
  • Application of yeast-derived dextran in the preparation of medicines for treating hepatitis B virus infection

Examples

Experimental program
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Effect test

Embodiment 1

[0018] (1) Cell culture, clinical specimen intervention and index detection plan

[0019] Isolate HBVC57BL / 6J-TgN transgenic mice (the mouse was introduced from the Jackson laboratory in the United States, contains HBVS, PreS1 and PreS2 genes, can express HBsAg in mouse serum) single spleen cells, DMEM culture medium (Gibco company, plus 10% fetal cattle Serum, 100U / ml penicillin, 100μg / ml streptomycin and 400μg / ml G418) in 5% CO 2 Incubate at 37°C in a saturated steam environment. Yeast-derived dextran 250μg / ml was stimulated for 48h, and cytokine expression was detected by quantitative PCR.

[0020] Separate HBVC57BL / 6J-TgN transgenic mouse bone marrow according to conventional methods, and induce differentiation of DC for 8 days with 100ng / mlIL-4 and 100ng / ml GM-CSF. DMEM medium (Gibco company, plus 10% fetal bovine serum, 100U / ml penicillin, 100μg / ml streptomycin and 400μg / ml G418) in 5% CO 2 Incubate at 37°C in a saturated steam environment. Take the logarithmic growth phas...

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Abstract

The invention belongs to the field of medicine, relates to an application of yeast-origin glucan on preparation of drugs for curing hepatitis B virus infection, and especially relates to an application on preparation of drugs for reducing or eliminating serum surface antigen of chronic hepatitis B virus infected patients. It has been testified by tests that the yeast-origin glucan can modulate the cell factor expression of mice spleen cells, increases the expressions of IL-12, IL-1, and TNF, and decreases the expression of inhibiting cell factor IL-10; can promote the maturity of mDC of HBV C57BL / 6J-TgN transgenic mice, and increases the expressions of mDC surface co-stimulatory molecules CD80 and CD 86; is capable of prominently strengthening the generation of peripheral blood-derived PBMC IFN-gamma of HBV infected patients. The yeast-origin glucan can be used for preparation of drugs for curing hepatitis B infection, especially preparations of drugs for reducing or eliminating serum surface antigen of chronic hepatitis B virus infected patients, and can be taken as an immunity strengthening agent to help the treatment of HBV infection.

Description

Technical field [0001] The invention belongs to the field of medicine, and relates to new medicinal uses of glucan, in particular to the application of yeast-derived glucan in the preparation of drugs for treating hepatitis B virus infection, especially in the preparation of serum for reducing or eliminating chronic hepatitis B virus infection Application of surface antigen in medicine. Background technique [0002] The prior art discloses that yeast-derived glucan (glucan) has a double-helix ultrastructure, and it interacts with human monocytes, macrophages, neutrophilegranulocytes, and dendrites. The CR3, LacCer, Dectin-1, TLR2 and other receptors on the surface of immune cell membranes such as dendritic cells (DC) and natural killer cells (NKcell) bind to the physiological functions of recognition and phagocytosis, and stimulate the body to produce antibodies and secrete TNF- Alpha, interleukins and interferons and other cytokines regulate the interaction between immune cells...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/716A61P1/16A61P31/20A61P37/04
Inventor 袁正宏羽晓瑜周晓辉任广旭
Owner SHANGHAI PUBLIC HEALTH CLINICAL CENT
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