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Plant polypeptide protein extract and use thereof

A technology of extract and peptide protein, applied in the field of plant polypeptide protein extract, can solve the problems of poor product stability, low extraction yield, activity loss, etc., and achieve the effect of stable product, high extraction efficiency and maintaining efficacy

Inactive Publication Date: 2013-12-18
GUANGZHOU DEVA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The prior art has the following shortcomings and deficiencies when extracting plant polypeptide proteins: low extraction yield, poor stability of the extracted product, easy deterioration, and serious loss of activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The present embodiment relates to a plant polypeptide protein extract, obtained through the following steps:

[0027] Step (1) taking the membranous bracts of snow lotus as plant raw materials, first coarsely crushing them, and then pulverizing them to obtain a pretreatment mixture of plant raw materials;

[0028] Step (2) soaking 3.0 parts by weight of the pretreatment mixture in 7.0 parts by weight of deionized water, and then keeping it sealed at 80° C. for 15 minutes to obtain a soaking mixture;

[0029] Step (3) adjust the pH value of the soaking mixture to 6.2, add protease, the mass ratio of protease to the pretreatment mixture in step (2) is 1.0%, then cool down to 40 ° C for hydrolysis reaction for 5h, centrifuge, precipitate, filter , stand for stratification to obtain clear liquid;

[0030] Step (4) Mix the clear liquid with glacial acetic acid in a mass ratio of 1:1.1 to [MORBSA][HSO 4 ] The ionic liquid is used as a catalyst to carry out the acetylation r...

Embodiment 2

[0032] The present embodiment relates to a plant polypeptide protein extract, obtained through the following steps:

[0033] Step (1) take Coix seed containing coarse skin as plant raw material, first coarsely crush and then pulverize to obtain a pretreatment mixture of plant raw materials;

[0034] Step (2) take 5.0 parts by weight of the pretreatment mixture and soak it in 5.0 parts by weight of deionized water, and then seal it at 85° C. for 10 minutes to obtain a soaking mixture;

[0035] Step (3) adjust the pH value of the soaking mixture to 6.8, add protease, the mass ratio of protease to the pretreatment mixture in step (2) is 0.1%, then cool down to 45 ℃ for hydrolysis reaction for 4h, centrifuge, precipitate, filter , stand for stratification to obtain clear liquid;

[0036] Step (4) Mix the clear liquid with glacial acetic acid in a mass ratio of 1:1.3 to [MORBSA][HSO 4 ] The ionic liquid is used as a catalyst to carry out the acetylation reaction, and the end grou...

Embodiment 3

[0038] The present embodiment relates to a plant polypeptide protein extract, obtained through the following steps:

[0039] Step (1) take rose petals as plant raw materials, first coarsely crush them, and then pulverize them to obtain a pretreatment mixture of plant raw materials;

[0040] Step (2) soaking 4.0 parts by weight of the pretreatment mixture in 6.0 parts by weight of deionized water, and then keeping it sealed at 82° C. for 13 minutes to obtain a soaking mixture;

[0041] Step (3) adjust the pH value of the soaking mixture to 6.4, add protease, the mass ratio of the protease to the pretreatment mixture in step (2) is 0.8%, then cool down to 42°C for hydrolysis reaction for 4.5h, centrifuge, precipitate, Filter, stand for stratification, and obtain clear liquid;

[0042] Step (4) Mix the clear liquid with glacial acetic acid in a mass ratio of 1:1.2 to [MORBSA][HSO 4 ] The ionic liquid is used as a catalyst to carry out the acetylation reaction, and the end group...

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PUM

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Abstract

The invention provides a plant polypeptide protein extract and use thereof. The extract is prepared from the following steps: taking one of saussurea involucrata membranous bract, red coix seed containing tertia, rose petals, barbados aloe, lavender petals, fruits of tea plants, seaweed leaves, zante currant and chamomile petals as a plant raw material; firstly, coarsely crushing and then crushing to obtain a pre-treatment mixture of the raw material; hermetically soaking the pre-treatment mixture in deionized water at 80-85 DEG C for 10-15 minutes; then adjusting the pH value to 6.2-6.8, adding protease and cooling to carry out hydrolysis reaction; centrifuging, participating, filtering, stewing and layering, so as to obtain clear liquid; carrying out shielding protection on a polypeptide protein end group in the clear liquid; desalting and concentrating to obtain the plant polypeptide protein extract. The plant polypeptide protein extract can be used as a cell activity factor and applied to foods, health-care drugs and cosmetics. The plant polypeptide protein extract has the advantages of being high in extraction efficiency, stable in extract, difficult to deteriorate, difficult to lose activity.

Description

technical field [0001] The present invention relates to a plant extract and use thereof, in particular, to a plant polypeptide protein extract and use thereof. Background technique [0002] With the improvement of plant extraction technology, more and more plant extracts are used. Among the many extracts, there is a class of substances containing polypeptide protein that people pay more and more attention to, because polypeptide protein affects the cells of human skin. Cosmetic efficacy has gradually been confirmed, and the application of polypeptide protein cell activity factors in the cosmetic industry is more and more extensive. The polypeptide proteins contained in different plants have different properties, which are also manifested in different cosmetic effects. [0003] When extracting plant polypeptide protein in the prior art, the following shortcomings and deficiencies exist: low extraction yield, poor stability of the extracted product, easy deterioration, and ser...

Claims

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Application Information

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IPC IPC(8): A61K8/97A61K8/64A61Q19/00
Inventor 史伟凯
Owner GUANGZHOU DEVA BIOTECH
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