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Tissue culture and rapid propagation method of cudrania tricuspidata

A fast technology of Zheshu, which is applied in the field of tissue culture and rapid propagation of Zheshu, can solve the problems of limited resources and other issues

Inactive Publication Date: 2013-12-18
NANJING TONGZE AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main chemical components in Zheshu medicinal materials are flavonoids and ketones, but their resources are limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Young leaves of Zhe tree taken from the field, soaked in washing powder for 3 minutes, rinsed with running water for 30 minutes, disinfected with 0.2% mercuric chloride on the ultra-clean workbench for 8 minutes, rinsed with sterile water 4-5 times, and inserted into the callus The tissue induction medium is WPM+IAA0.2mg / l+6-BA3mg / l, subcultured for 2-3 generations, inoculated into the differentiation medium WPM+IAA0.1mg / l+KT0.5mg / l+2mg / lABA, Induce differentiation for 30 days, and inoculate the differentiated Zheshu seedlings into WPM+0.2mg / LNAA+coconut milk+5mg / LAgNO 3 , coconut milk can promote the growth of organs, AgNO 3 It is used to prevent vitrification of test-tube seedlings and premature fading of leaves. The 5cm-long Zheshu seedlings are first placed in the cultivation room, the sealing film is removed, and the open mouth is left for a week. After a week, the root culture medium is washed away with clean water, put in sand, and placed In a sterile light incu...

Embodiment 2

[0011] Young leaves of Zhe tree taken from the field, soaked in washing powder for 3 minutes, rinsed with running water for 30 minutes, disinfected with 0.2% mercuric chloride on the ultra-clean workbench for 8 minutes, rinsed with sterile water 4-5 times, and inserted into the callus Tissue induction medium is WPM+IAA0.2mg / l+6-BA3mg / l, subculture for 2-3 generations, inoculate into differentiation medium WPM+IAA0.3mg / l+KT1.5mg / l+2mg / l ABA , induced differentiation for 30 days, and the differentiated Zhessia seedlings were inoculated into WPM+0.5mg / LNAA+coconut milk+5mg / LAgNO 3 , coconut milk can promote the growth of organs, AgNO 3 It is used to prevent vitrification of test-tube seedlings and premature fading of leaves. The 5cm-long Zheshu seedlings are first placed in the cultivation room, the sealing film is removed, and the open mouth is left for a week. After a week, the root culture medium is washed away with clean water, put in sand, and placed In a sterile light incu...

Embodiment 3

[0013] Young leaves of Zhe tree taken from the field, soaked in washing powder for 3 minutes, rinsed with running water for 30 minutes, disinfected with 0.2% mercuric chloride on the ultra-clean workbench for 8 minutes, rinsed with sterile water 4-5 times, and inserted into the callus Tissue induction medium is WPM+IAA0.2mg / l+6-BA3mg / l, subculture for 2-3 generations, inoculate into differentiation medium WPM+IAA0.2mg / l+KT1.5mg / l+2mg / l ABA , induced differentiation for 30 days, and the differentiated Zhe tree seedlings were inoculated into WPM+0.3mg / LNAA+coconut milk+5mg / LAgNO 3 , coconut milk can promote the growth of organs, AgNO 3 It is used to prevent vitrification of test-tube seedlings and premature fading of leaves. The 5cm-long Zheshu seedlings are first placed in the cultivation room, the sealing film is removed, and the open mouth is left for a week. After a week, the root culture medium is washed away with clean water, put in sand, and placed In a sterile light inc...

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Abstract

The invention discloses a tissue culture and rapid propagation method of cudrania tricuspidata. The tissue culture and rapid propagation method comprises the steps of acquisition of sterile leaves, callus induction, callus differentiation, strong seedling culture, domestication and transplanting and the like. By adopting a complete tissue culture system of the cudrania tricuspidata established by the tissue culture and rapid propagation method, the technical difficult problems that the cudrania tricuspidata is low in growth speed and low in propagation rate and the like are solved, and the requirements on high proliferation coefficient and high survival rate are met. An artificial cultivation method which is short in cycle and high in propagation rate can be provided for the industrialized production of the cudrania tricuspidata.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for tissue culture and rapid propagation of chrysanthemum. Background technique [0002] Zhe tree (Cudrania tricuspidata (Carr.) Bur.) is a plant of the genus Cudrania tricuspidata (Carr.) Bur., also known as Cudrania tricuspidata (Carr.) Bur. Deciduous shrubs or small trees, which like to grow in sunny barren mountains, slopes, hills and beside water streams, are widely distributed in East China, Central South, Southwest to southern Hebei. The roots, bark or root bark, stems and leaves, and fruits of the tree can be used as medicine. It has the effects of dispelling wind and dampness, promoting blood circulation and dredging menstruation, relieving cough and reducing phlegm, and can treat rheumatic joint pain, jaundice, stranguria, amenorrhea, and traumatic injuries Injuries and boils and carbuncles have been used in the treatment of digestive system tumors such as esophag...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨存
Owner NANJING TONGZE AGRI SCI & TECH
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