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Alkaline-tolerant and halophilic aspergillus strain and application thereof in environmental management

A technology of Aspergillus grisea and strains, applied in application, fungi, other chemical processes, etc., can solve problems such as accelerating straw, and achieve the effect of efficient degradation

Inactive Publication Date: 2013-12-11
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microbial community in saline-alkali land, especially in severe saline-alkali soil, is extremely simple and inactive. Although adding certain bacterial preparations can accelerate the degradation of straw during the process of returning straw to the field, increase the organic matter content in the soil, and significantly improve soil physical and chemical properties. It has obvious effects on maintaining a good ecological environment system and crop nutrition system in farmland, reducing environmental pollution, increasing yield and high quality, etc. However, the research team found that these bacterial agents can play a good role in normal soil, but in saline-alkali soil In the high concentration of salt and high pH environment, it is difficult for these strains to play their due role

Method used

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  • Alkaline-tolerant and halophilic aspergillus strain and application thereof in environmental management
  • Alkaline-tolerant and halophilic aspergillus strain and application thereof in environmental management
  • Alkaline-tolerant and halophilic aspergillus strain and application thereof in environmental management

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1, Isolation and identification of halophilic and alkali-resistant bacterial strains

[0018] Isolation of the strains: The soil samples for the isolation of the strains were from Zhenlai County, Jilin Province. Select the land that has been desolate for many years and is highly salinized, take the soil below 2cm of the soil surface, put it into a sterilized glass bottle, and bring it back to the laboratory for later use. Using the dilution coating method, the diluted and clarified soil supernatant was spread on the PDA medium (pH=10) supplemented with NaCl to 250.0g / L and cultured for 4 days. After three rounds of screening, a halophilic and resistant Alkaline fungal strains (the alkali-tolerant halophilic Aspergillus cinerea strain number of the present invention is CGMCC6025).

[0019] Morphological identification of strains: Inoculate the screened halophilic and alkali-resistant strains on Chase agar medium, culture at 25°C for one week, observe the colony ...

Embodiment 2

[0020] Embodiment 2, bacterial strain Aspergillus cinerea JL halophilicity, salt tolerance and optimum salt concentration identification

[0021] The PDA medium (pH 7.0) with gradient NaCl content was prepared, and its salt concentration (mass fraction) was increased from 1% to 31% (saturation concentration), with an increase of 2% at each level. The PDA without NaCl was used as the control. Inoculate an equal amount of strains in the center of the culture medium, culture at 25°C for 1 week. It was found that the strain did not grow on the medium without NaCl (growth was extremely slow), and the growth was relatively slow at the concentration of 1-5%; the growth of the strain was accelerated at the concentration of 5%-19%, but in the range of 9-19% The growth rate slows down, and the size of the colony is similar; the growth of the strain slows down sharply at the concentration of 21-27%, and the bacteria hardly grows at the concentration of 29-31%. Therefore, the strain is a ...

Embodiment 3

[0023] Embodiment 3, bacterial strain Aspergillus gray aeruginosa JL growth pH measurement

[0024] Prepare an acid-base gradient PDA medium with a pH of 1.0-13.0, add NaCl to a final concentration (mass fraction) of 9%, inoculate, and record the growth of the strain after culturing at 25°C for 1 week. The study found that the strain can grow at pH 3-11, but grows faster at pH 5-9, and can grow at pH 10-12, but the growth rate is significantly slower. Therefore, the strain is a salt-tolerant fungus.

[0025] In order to determine the optimum pH range for the growth of the strain, use the above pH gradient to further culture with PD liquid medium, shake culture at 25°C for 1 week, collect mycelia by centrifugation, rinse with deionized water for 4 times, and dry at 105°C , to compare the dry weight of mycelia. The bacterial strain has the largest dry weight in the range of pH6-8, therefore, the optimum pH range of the bacterial strain is 6-8.

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Abstract

The invention belongs to the field of microbial engineering and technology, and specifically relates to an alkaline-tolerant and halophilic aspergillus strain and an application thereof in environmental management. The strain is preserved in China General Microbiological Culture Collection Center on April 20th, 2012, with a preservation name of aspergillus JL and a preservation number of CGMCC 6025. The strain has the characteristic of degrading biological substances such as straws in a high salt and alkali environment and can be used for improvement of saline lands and treatment of environmental pollutions.

Description

Technical field: [0001] The invention belongs to the field of microbial engineering and technology. In particular, it relates to an Aspergillus cinerea strain with alkali-resistant and halophilic properties, and its application in saline-alkali land improvement and environmental management. Background technique: [0002] Microorganisms are organisms that live in a wide range of environments in nature. Regardless of prokaryotic or eukaryotic organisms, no matter how harsh the environmental conditions are, they can be found almost everywhere on the earth. Halophilic or (and) alkali-tolerant fungi are microbial resources isolated from high-salt, high-alkaline or high-salt-alkali coexistence environments. Due to their important research, application and development value, they have always attracted attention. At present, with the discovery of microbial resources of halophilic and alkali-resistant bacteria, people are beginning to apply these halophilic and alkali-resistant bact...

Claims

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Application Information

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IPC IPC(8): C12N1/14C09K17/00C12R1/66C09K101/00C09K109/00
Inventor 魏毅刘晓丹贾保磊张世宏刘金亮高文彬
Owner JILIN UNIV
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