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Optimized formula and preparation process for W2000 liquid stock of mushroom breathing bag cultivated species

A breathing bag, cultivar technology, applied in horticulture, botanical equipment and methods, fertilizer mixtures, etc., can solve the problems of slow inoculation speed, insufficient loose agglomeration, uneven bacteria removal, etc., to improve production efficiency and avoid agglomeration Problem, effect that inoculation is easy

Active Publication Date: 2013-12-11
INST OF EDIBLE FUNGI FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of optimized formula and preparation process of the W2000 liquid stock that can replace the solid stock for the production of mushroom breathing bag cultivars. The W2000 liquid seed with strong vitality was prepared to replace the solid seed for the production of breathing bag cultivars, which can solve the pollution and inoculation speed of breathing bag cultivars caused by the hidden pollution, large particles, and insufficient agglomeration of solid stock seeds. Slow, uneven bacteria removal and other problems, thereby further improving the production efficiency and process level of breathing bag cultivars and reducing production costs

Method used

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  • Optimized formula and preparation process for W2000 liquid stock of mushroom breathing bag cultivated species

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Preparation of W2000 mother liquor strains

[0036] a. Accurately weigh 150g potatoes, 75g bran, 15g brown sugar, 3g peptone, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.13g;

[0037] b. Add 0.45 L of distilled water to the potatoes and bran, heat to boiling, filter with four layers of gauze after 20 minutes, discard the potato residue and bran residue, and obtain the potato and bran mixed filtrate for later use.

[0038] c, add 3g KH to the mixed filtrate 2 PO4 and 1.13g MgSO 4 ·7H 2 O, stir evenly, pour into a 2L graduated cylinder, make up the mixed solution to 1.5L with distilled water, which is the mother liquor culture medium;

[0039] d. Pour 100mL mother liquor culture medium into a 250mL conical flask with a 100mL graduated cylinder, and plug the bottle with a silica gel stopper;

[0040] e. 0.1Mpa, 121°C high-pressure steam sterilization for 20min;

[0041] f. After the mother liquid medium is cooled, put it in the ultra-clean workbench and irradiate it with u...

Embodiment 2

[0061] 1. Preparation of W2000 mother liquor

[0062] a. Accurately weigh 200g potatoes, 100g bran, 20g brown sugar, 4g peptone, KH 2 PO 4 4g, MgSO 4 ·7H 2 O 1.5g;

[0063] b. Add 0.6L of distilled water to the potatoes and bran, heat to boiling, filter with four layers of gauze for 20 minutes, discard the potato residue and bran residue, and obtain the potato and bran mixed filtrate for later use.

[0064] c, add 4g KH to the mixed filtrate 2 PO 4 and 1.5g MgSO 4 ·7H 2 O, stir evenly, pour into a 2L graduated cylinder, make up the mixed solution to 2L with distilled water, which is the mother liquor culture medium;

[0065] d. Pour 100mL mother liquor culture medium into a 250mL conical flask with a 100mL graduated cylinder, and plug the bottle with a silica gel stopper;

[0066] e. 0.1Mpa, 121°C high-pressure steam sterilization for 20min;

[0067] f. After the mother liquid medium is cooled, put it in the ultra-clean workbench and irradiate it with ultraviolet li...

Embodiment 3

[0083] 1) Preparation of W2000 mother liquor:

[0084] a. Preparation of mother liquor culture medium;

[0085] Accurately weigh 100g potato, 50g bran, 10g brown sugar, 2g peptone, KH 2 PO 4 2g, MgSO 4 ·7H 2 O 0.75g; Add 0.3L distilled water to potatoes and bran and heat to boiling, filter with four layers of gauze for 20min, discard potato residue and bran residue, and obtain potato and bran mixed filtrate for subsequent use. Add 2.0 g KH to the combined filtrate 2 PO 4 and 0.75g MgSO 4 ·7H 2 O, stir evenly, pour into a 1L graduated cylinder, make up the mixed solution to 1L with distilled water, which is the mother culture medium.

[0086] Measure 100mL of liquid seed medium in a 100mL graduated cylinder and pour it into a 250mL triangular flask with a silicone stopper; 0.1Mpa, 121°C high-pressure steam sterilization for 20min; after the mother liquor medium is cooled, place it in an ultra-clean workbench and irradiate it with ultraviolet light for 30min.

[0087] ...

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Abstract

The invention discloses an optimized formula and a preparation process for a W2000 liquid stock of a mushroom breathing bag cultivated species. The formular of a culture medium of the W2000 liquid stock comprises the following components in percentage by weight: 38-48 g / L of corn starch, 10-15 g / L of soybean meal, 2.5-3.5 g / L of KH2PO4 and 0.8-1.2 g / L of MgSO4.7H2O; the culture medium is cultured under the proper culture conditions that the fermentation temperature is 22-25 DEG C, the rotating speed of a shake flask is 180-220 r / min, the initial pH is 6.3-6.6 and the fermentation time is 6.5-7.5 days; the preparation process of the W2000 liquid stock comprises the steps: preparing a mother solution culture medium, connecting a PDA (Potato Dextrose Agar) stock, carrying out shaking culture, preparing a W2000 mother solution strain, preparing a liquid stock culture medium, connecting into the mother solution, carrying out shaking culture and preparing the W2000 liquid stock. The W2000 liquid stock can replace a solid stock to be connected into a mushroom breathing bag cultivated species substrate for producing a W2000 cultivated species. The W2000 liquid stock prepared by the optimized formula and the preparation process which are disclosed by the invention has the advantages of short culture period, low cost, strong activity, consistent fungus age and the like.

Description

technical field [0001] The invention belongs to the technical field of edible fungus strains, and in particular relates to an optimized formula and a preparation process of a W2000 mushroom breathing bag cultivar liquid stock. Background technique [0002] Agaricus bisporus is an edible fungus produced and consumed worldwide. The annual output of Agaricus bisporus in my country accounts for more than 50% of the world, reaching 2.46 million tons in 2012, with an output value of more than 10 billion yuan. In recent years, due to the increase in labor costs, the factory planting of Agaricus bisporus has become a trend in the development of mushroom cultivation. The Institute of Edible Fungi of the Fujian Academy of Agricultural Sciences is the only scientific research unit in China that focuses on the research and development of Agaricus bisporus strain breeding and seed production technology. The selected As2796 mushroom strain has been the main species of mushroom cultivation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00A01G1/04
Inventor 戴建清曾志恒曾辉程翊杨雷王泽生
Owner INST OF EDIBLE FUNGI FUJIAN ACAD OF AGRI SCI
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