Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of cytoplasm Ascorbate PeroXidase as plant drought resisting marker

A peroxidase, ascorbic acid technology, applied in the fields of biotechnology and botany, can solve long and difficult problems

Inactive Publication Date: 2013-12-04
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Screening plants with strong drought resistance is an aspect that those skilled in the art have been working on. However, it is long and difficult to conduct research by traditional research methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of cytoplasm Ascorbate PeroXidase as plant drought resisting marker
  • Application of cytoplasm Ascorbate PeroXidase as plant drought resisting marker
  • Application of cytoplasm Ascorbate PeroXidase as plant drought resisting marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Embodiment 1, comparing different periods plants are affected by drought

[0104] Firstly, the effects of Zhonghan 3 and IR29 on yield under the same drought stress conditions were examined.

[0105] figure 1 A The left picture shows the water use efficiency (WUE) of the two varieties at the seedling stage, and the right picture shows the dry weight of the aboveground part (dry matter accumulation) measured after the seedlings have been cut off from water around to severe leaf curling, indicating that the two varieties are sensitive to water shortage at the seedling stage responses did not differ significantly.

[0106] However, the yield of the two varieties decreased significantly when drought stress was applied during panicle development. Such as figure 1 B, Compared with their respective plants grown under the same conditions but without drought treatment (Con), the seed setting rate of Zhonghan 3 and IR29 decreased by 39% and 68%, respectively, and the yield per...

Embodiment 2

[0108] Embodiment 2, changes in protein expression after drought treatment

[0109]During the reproductive growth period, Zhonghan 3 and IR29 were treated with water cut-off and drought, and their control (control, culture conditions were the same but without drought treatment) and drought panicle samples were taken respectively. Grinding with liquid nitrogen, lysing the sample after crude protein extraction by TCA / acetone method, and quantifying the protein by Bradford method, each took 150 μg for two-dimensional electrophoresis experiment, and the obtained two-dimensional gel was transferred to membrane and hybridized with anti-APX antibody (AS08 368, Agrisera, Sweden) , ECL chromogenic tablet. A total of 11 APX isoforms were detected by 2D-WB, of which 6 isoforms (that is, the isoforms corresponding to the positions of protein spots 1, 2, 3, 7, 8, and 9 mentioned later) were detected in Zhonghan 3 were induced by stress, while only one of these isoforms was expressed at a ...

Embodiment 3

[0117] Embodiment 3, research on the expression of APX isomers in other varieties

[0118] The above results suggest that APX has species-specificity in rice panicle response to drought stress, such as image 3 The shown APX2 (protein point 1 (molecular weight 29kD, isoelectric point 4.8), 2 (molecular weight 29.52kD, isoelectric point 4.92), 3 (molecular weight 30kD, isoelectric point 4.98)), APX1 (protein point 7 (molecular weight 26.5 kD, isoelectric point 5.15), 8 (molecular weight 27.2kD, isoelectric point 5.3), 9 (molecular weight 27.8kD, isoelectric point 5.33)). The present inventors further verified whether APX1 protein isoforms 1, 2, and 3 and APX2 protein isoforms 7, 8, and 9 can be used as drought resistance marker proteins in more rice varieties.

[0119] The typical rice Zhenshan 97B, the typical upland rice IRAT109, the relatively drought-resistant variety C22 and the drought-sensitive variety IRAT106 were treated with water cut-off and drought during the repro...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an application of cytoplasm Ascorbate PeroXidase as a plant drought resisting marker, and provides a marker about plant drought resistance: cytoplasm Ascorbate PeroXidase (APX). When plants with strong drought resistance are compared to drought sensitive plants, the cytoplasm Ascorbate PeroXidase shows low expression in the plants with strong drought resistance, but after the plants with strong drought resistance are subjected to drought stress, the expression quantity is increased observably. Therefore, the cytoplasm Ascorbate PeroXidase can be used in indication of plant drought resistance accurately and provides a new way for cultivation of excellent plant varieties.

Description

technical field [0001] The invention belongs to the fields of biotechnology and botany; more specifically, the invention relates to the application of cytoplasmic ascorbate peroxidase (APX) as a plant drought resistance marker. Background technique [0002] Drought is the most important abiotic stress affecting crop production and an important environmental problem facing the world. Grasses, especially rice, are the most important food crops in the world. More than half of the world's population uses rice as a staple food, especially in developing countries in Asia. However, rice requires more water than other food crops such as wheat and corn. In Asia, 70% of agricultural water is used for rice production. In order to solve this contradiction, it is necessary to adjust the farming system and strengthen water-saving irrigation. However, enhancing the drought resistance of rice itself and selecting rice with strong drought resistance are the most critical. [0003] Screeni...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573
Inventor 孙卫宁黄蔚
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com