Preparation method and application of patrinia villosa root extractive

A technology of Patrinia officinalis and its extract, which is applied in the field of health food processing, can solve the problems that there are few studies on the pharmacological activity of Patrinia officinalis root, and there are no reports on the antioxidant activity of Patrinia officinalis root extract.

Inactive Publication Date: 2013-12-04
CHONGQING TECH & BUSINESS UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are few studies on the pharmacological activity of Patrinia root, and there is no report on the antioxidant activity of Patrinia root extract

Method used

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  • Preparation method and application of patrinia villosa root extractive

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Comparison scheme
Effect test

Embodiment 1

[0016] 1, a kind of preparation method and the application thereof of Bai Hua Patrin root extract, it is characterized in that comprising the following steps:

[0017] (1) Take the root of Baihuabaijiang, wash it with water, dry it or dry it in the air, crush it, soak it in 10 times, 8 times, and 6 times the amount of 75% ethanol for 8 hours, and then ultrasonicate it for 1 hour at 30°C and 80W power ;

[0018] (2) Standing and filtering the solution in step (1), and recovering ethanol under reduced pressure with a rotary evaporator to obtain a crude extract;

[0019] (3) Dilute the liquid extract in step (2) with an appropriate amount of water to form a suspension, then extract three times with 4 times the amount of petroleum ether and ethyl acetate, and combine the ethyl acetate layer extract;

[0020] (4) Concentrate the ethyl acetate layer extract in step (3) under reduced pressure, and freeze-dry to constant weight to obtain the ethyl acetate extract, which is set aside....

Embodiment 2

[0023] 1, a kind of preparation method and the application thereof of Bai Hua Patrin root extract, it is characterized in that comprising the following steps:

[0024] (1) Take the root of Baihuabaijiang, wash it with water, dry or dry it in the air, crush it, soak it in 10 times, 8 times, and 6 times the amount of 75% ethanol for 10 hours, and then ultrasonicate for 1 hour at a temperature of 30°C and a power of 80W ;

[0025] (2) Standing and filtering the solution in step (1), and recovering ethanol under reduced pressure with a rotary evaporator to obtain a crude extract;

[0026] (3) Dilute the liquid extract in step (2) with an appropriate amount of water to form a suspension, then extract three times with 5 times the amount of petroleum ether and ethyl acetate, and combine the ethyl acetate layer extract;

[0027] (4) Concentrate the ethyl acetate layer extract in step (3) under reduced pressure, and freeze-dry to constant weight to obtain the ethyl acetate extract, wh...

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Abstract

The invention belongs to the field of health food processing technologies, and relates to a preparation method and application of a patrinia villosa root extractive. 75% ethyl alcohol is used for performing digestion on dried patrinia villosa root powder at the room temperature, then ultrasonic extraction is performed, standing and filtering are performed, and reduced pressure distillation is performed on filter liquor, so that crude extract is obtained; appropriate water is added to the crude extract to dilute the crude extract to be a suspension, extraction is performed for three times with petroleum ether and ethyl acetate, ethyl acetate layer extraction liquor is combined, reduced pressure condensation is performed on the ethyl acetate layer extraction liquor, then the ethyl acetate layer extraction liquor is dried to constant weight in a freeze mode, and the ethyl acetate extractive is obtained for use. The patrinia villosa root extractive has good capacity for clearing DPPH free radicals and ABTS free radicals. The method of the patrinia villosa root extractive can be used for developing new antioxidant in the fields of food, medicine, health food and the like, and is beneficial to industrial production.

Description

technical field [0001] The invention relates to a preparation method and application of an extract of Patrinia japonicus root, belonging to the technical field of health food processing. The invention can be used to develop new natural antioxidants in the fields of food, medicine, health products and the like, and the product of the invention is beneficial to industrial production. Background technique [0002] Free radicals are by-products of cell metabolism in the body, and these compounds are a class of chemical substances that are active and have strong oxidative capacity. Studies have shown that free radicals produced in large quantities in organisms, such as superoxide anion free radicals and hydroxyl free radicals, can cause DNA damage, protein oxidation and lipid peroxidation. Excessive production of free radicals in organisms has been associated with many clinical diseases, such as heart disease, diabetes, liver damage, cancer, and aging. When innate defenses are i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P39/06A23L1/30A61K125/00A23L33/105
Inventor 高雪陈刚孔淑贞
Owner CHONGQING TECH & BUSINESS UNIV
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