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Triple nucleic acid detection kit for neisseria gonorrhoeae / ureaplasma urealyticum/ chlamydia trachomatis

A detection kit, the technology of Ureaplasma urealyticum, applied in the biological field, can solve the problems of low sensitivity and poor specificity, and achieve the effects of high sensitivity, pollution prevention, rapid and objective detection results

Active Publication Date: 2015-05-13
JIANGSU BIOPERFECTUS TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The technical problem mainly solved by the present invention is to overcome the defects such as poor specificity and low sensitivity of existing gonorrhea / ureaplasma urealyticum / chlamydia trachomatis detection kits, and provide gonorrhea / ureaplasma urealyticum / chlamydia trachomatis, which can effectively To prevent pollution, it has the advantages of high sensitivity, good specificity, strong repeatability, rapid and objective test results, etc. It has a good application prospect for the detection of gonorrhea / ureaplasma urealyticum / chlamydia trachomatis

Method used

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  • Triple nucleic acid detection kit for neisseria gonorrhoeae / ureaplasma urealyticum/ chlamydia trachomatis
  • Triple nucleic acid detection kit for neisseria gonorrhoeae / ureaplasma urealyticum/ chlamydia trachomatis
  • Triple nucleic acid detection kit for neisseria gonorrhoeae / ureaplasma urealyticum/ chlamydia trachomatis

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Embodiment 1

[0051] Example 1 A nucleic acid detection kit for rapid detection of Neisseria gonorrhoeae / Ureaplasma urealyticum / Chlamydia trachomatis.

[0052] The above kit includes RT-PCR reaction solution, enzyme mixture, Neisseria gonorrhoeae (NG) / Ureaplasma urealyticum (UU) / Chlamydia trachomatis (CT) triple reaction solution, positive control and negative control;

[0053] Wherein, the PCR reaction solution includes 10× buffer, 25mM MgCl 2 , 10mM dUTP and 10mM dNTPs;

[0054] The triple reaction liquid of gonorrhoeae / ureaplasma urealyticum / chlamydia trachomatis comprises the following components:

[0055] Component (1): composed of a pair of primers for detecting Neisseria gonorrhoeae and a probe for detecting Neisseria gonorrhoeae; wherein, the base sequences of the two primers are respectively shown in SEQ ID No.1 and SEQ ID No.2; The base sequence of the needle is shown in SEQ ID No.3, the 5' end of the probe is marked with a fluorescent reporter group, and the 3' end is marked wi...

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Abstract

The invention discloses a triple nucleic acid detection kit for neisseria gonorrhoeae / ureaplasma urealyticum / chlamydia trachomatis. The kit comprises an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) reaction liquid, an enzyme mixed liquid, a triple reaction liquid for neisseria gonorrhoeae / ureaplasma urealyticum / chlamydia trachomatis, positive control and negative control. The kit disclosed by the invention overcomes the deficiencies that in the prior art, neisseria gonorrhoeae / ureaplasma urealyticum / chlamydia trachomatis is poor in specificity, lower in sensitivity and the like, effectively prevents pollution, has the advantages of high sensitivity, good specificity, strong repeatability, quick and objective detection result and the like, and has good application prospect for detecting neisseria gonorrhoeae / ureaplasma urealyticum / chlamydia trachomatis.

Description

technical field [0001] The invention relates to a nucleic acid detection kit, in particular to a triple nucleic acid detection kit for Neisseria gonorrhoeae / Ureaplasma urealyticum / Chlamydia trachomatis, belonging to the field of biotechnology. Background technique [0002] STDs are a group of diseases that are mainly transmitted through sexual contact. Sexually transmitted diseases are the most serious and widespread infectious diseases that endanger human beings. They not only endanger personal health, but also bring disaster to families, future generations, and society at the same time. Sexually transmitted diseases damage human health in many ways. If sexually transmitted diseases cannot be detected and treated thoroughly after infection, not only can the reproductive organs be damaged, leading to infertility, some sexually transmitted diseases can also damage the heart, brain and other vital organs of the human body, and even lead to death. Some sexually transmitted di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/04G01N21/64
Inventor 王国强刘中华李荣宇方存磊张旭
Owner JIANGSU BIOPERFECTUS TECH CO LTD
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