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Low-molecular-weight Tremella aurantialba polysaccharide and preparation method thereof

A golden ear polysaccharide, low molecular weight technology, applied in skin care preparations, cosmetics, etc., can solve the problems of affecting the expression of biological activity, large molecular volume, poor water solubility, etc., and achieve the effect of improving moisturizing activity and simple preparation method

Inactive Publication Date: 2013-11-27
SHANGHAI JAHWA UNITED +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the polysaccharides obtained from golden ears are undegraded polysaccharides, which have large molecular weight, large molecular volume, and poor water solubility, which is not conducive to bioabsorption, thereby affecting the expression of their biological activities in the human body, which greatly limits the polysaccharides. application

Method used

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  • Low-molecular-weight Tremella aurantialba polysaccharide and preparation method thereof
  • Low-molecular-weight Tremella aurantialba polysaccharide and preparation method thereof
  • Low-molecular-weight Tremella aurantialba polysaccharide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Preparation of large molecular weight auricularia polysaccharide by water extraction and alcohol precipitation method

[0024] Grind the fruiting body of golden ear (purchased from Yunnan Tianzhiyao Wild Edible Fungi Co., Ltd.) into powder with a grinder, add water according to the ratio of 100g fruiting body to 10L water, heat and boil for 2h, then centrifuge with a centrifuge, filter the residue and repeat the above The step is to extract once more and centrifuge, and combine the filtrates of the two extractions. After the filtrate was concentrated to 2L, 3L of absolute ethanol was added for precipitation, and the precipitate was washed three times with 80% ethanol, and heated and dried in an oven at 80° C. to obtain Auricularia polysaccharide. Yield: 40.10%; polysaccharide content: 61.43%.

[0025] The golden ear polysaccharide that obtains here is through HPLC analysis, and molecular weight is about 6,800,000 Da ( figure 1 ). Example 2 Degradation of Au...

Embodiment 2

[0025] The golden ear polysaccharide that obtains here is through HPLC analysis, and molecular weight is about 6,800,000 Da ( figure 1 ). Example 2 Degradation of Auricularia polysaccharide by dilute acid

[0026] Weigh 10g of the dried golden ear polysaccharide of about 6.8 million Da in Example 1, dissolve it in 1L of 0.5N HCI aqueous solution, place it in a water bath at 80°C for 4 hours, and neutralize it with 0.5N NaOH solution to neutral after the degradation , and then precipitate with ethanol (the volume concentration of ethanol in the solution is 80%), and the precipitation is washed three times with ethanol (the volume concentration of ethanol in the solution is 80%).

[0027] Dissolve the acid-hydrolyzed Auricularia polysaccharide precipitate in water, concentrate and freeze at -20°C for 3 hours, then freeze at -80°C for 6 hours, then transfer to a freeze dryer for freeze-drying, and finally obtain a white low molecular weight Golden ear polysaccharide. Yield: 53...

Embodiment 3

[0028] This low-molecular-weight golden ear polysaccharide is analyzed by HPLC, and the molecular weight is about 12,000 Da ( figure 2 ). Example 3 In vitro moisturizing activity experiment of low molecular weight golden ear polysaccharide

[0029] 1.1 Determination of moisturizing activity in vitro

[0030] will be saturated by (NH4) 2 SO 4 The aqueous solution is used as a desiccator with a relative humidity (R.H.) of 81% and a saturated K 2 CO 3 The aqueous solution was placed in a constant temperature environment of 20° C. as a desiccator with a relative humidity (R.H.) of 43%.

[0031] Accurately weigh 1.0g of various samples and place them in a crystallization dish with a diameter of 3cm, and place them in a desiccator with a relative humidity (R.H.) of 81%. The weight difference, the moisture absorption rate is obtained from formula (1), WO is the weight of the sample before placing it, and Wn is the weight after placing it for 48 hours.

[0032] Moisture absorp...

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Abstract

The invention provides a low-molecular-weight Tremella aurantialba polysaccharide and a preparation method thereof. The low-molecular-weight Tremella aurantialba polysaccharide is prepared by water extraction, alcohol precipitation, acidolysis and alcohol re-precipitation. The preparation method provided by the invention is simple and suitable for large-scale production; the content of the low-molecular-weight Tremella aurantialba polysaccharide can reach more than 80%, and the yield is up to more than 50%; and the moisturizing activity of the Tremella aurantialba polysaccharide is obviously enhanced.

Description

technical field [0001] The invention relates to the field of edible fungi, in particular to a low-molecular-weight golden ear polysaccharide and a preparation method thereof. Background technique [0002] Golden ear, commonly known as brain ear and golden fungus, is a rare and precious edible fungus. In recent years, edible fungi have been used more and more in cosmetics, especially edible fungus polysaccharides are natural moisturizers, which can effectively improve the moisturizing ability of cosmetics, and golden ear polysaccharides are one of the main active ingredients of golden ear The study of polysaccharides with moisturizing effect has a good application prospect. [0003] Golden ear polysaccharide is mainly used in food and cosmetic industry. Studies in recent years have shown that the polysaccharides of golden ear polysaccharides have relatively good biological activities, such as anti-tumor, improving immunity, and lowering blood sugar, so it has a wide applica...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61Q19/00
Inventor 冯冰张忠马来记吴越唐庆九贾薇吴迪唐传红
Owner SHANGHAI JAHWA UNITED
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