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Anti-adhesion biological membrane and preparation method thereof

A biofilm and anti-adhesion technology, which is applied in the field of tissue engineering medical biomaterials, can solve the problems of inability to suppress inflammation and achieve good physical barrier anti-adhesion, good biocompatibility, and not easy to fall off.

Active Publication Date: 2013-11-27
XIAN TISSUE ENG & REGENERATIVE MEDICINE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to reports, the Seprafilm hyaluronic acid film produced by Genzyme Biosurgery, which is widely used at home and abroad, is used to prevent postoperative adhesions, and the results show that the effective rate is only 47% (Qiqiang Zeng, et, al. World J Surg (2007) 31:2125–2131), this product is prepared from a single natural polysaccharide polymer material, which can only play a preliminary physical barrier role, and cannot achieve the effects of inhibiting inflammation, inhibiting fibrosis, and promoting healing

Method used

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  • Anti-adhesion biological membrane and preparation method thereof
  • Anti-adhesion biological membrane and preparation method thereof
  • Anti-adhesion biological membrane and preparation method thereof

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Embodiment 1

[0031] Step 1. Disinfection treatment: wash the flake SIS with the mucous membrane layer and lymph nodes removed with purified water until there is no dirt, soak it in an aqueous sodium hydroxide solution with a concentration of 0.5mol / L for half an hour, and then wash it with purified water to pH neutral;

[0032] Step 2. Decellularization and antigen removal treatment: soak the sterilized SIS in a trypsin solution with a concentration of 1 mg / mL for half an hour, then wash it with purified water, and place it in a DNA hydrolase solution containing 25 U / mL and 40 U / mL α-galactosidase enzyme solution for 4 hours, and finally washed 10 times with purified water;

[0033] Step 3: Composite hyaluronic acid: Cut the SIS processed in step 2 to 3×4cm, soak it in a hyaluronic acid solution with a concentration of 20 mg / mL for 2 hours, take it out and freeze-dry it in vacuum to obtain a compounded hyaluronic acid SIS diaphragm;

[0034] Step 4. Sterilization treatment: after packagi...

Embodiment 2

[0038] Step 1. Disinfection treatment: wash the flake SIS with the mucous membrane layer and lymph nodes removed with purified water until it is free of dirt, soak it in an aqueous sodium hydroxide solution with a concentration of 3 mol / L for 2.5 hours, and then wash it with purified water to pH neutral;

[0039] Step 2. Decellularization and antigen removal treatment: soak the sterilized SIS in a trypsin solution with a concentration of 1mg / mL for half an hour, then wash it with purified water and place it in a solution containing 10U / mL DNA hydrolase and 30U / mL Soak in the α-galactosidase enzyme solution for half an hour, and finally wash 5 times with purified water;

[0040] Step 3: Composite hyaluronic acid: Cut the SIS processed in step 2 to 4×6 cm, soak it in a hyaluronic acid solution with a concentration of 5 mg / mL for half an hour, take it out, and vacuum freeze-dry it to obtain a compounded hyaluronic acid Acidic SIS diaphragm;

[0041] Step 4. Sterilization treatm...

Embodiment 3

[0045] Step 1. Disinfection treatment: wash the sheet-shaped SIS with the mucous membrane layer and lymph nodes removed with purified water until it is free of dirt, soak it in an aqueous potassium hydroxide solution with a concentration of 1 mol / L for 1 hour, and then wash it with purified water to pH neutral;

[0046] Step 2. Decellularization and antigen removal treatment: soak the sterilized SIS in a trypsin solution with a concentration of 3 mg / mL for 2 hours, wash with purified water, and place in a solution containing 15 U / mL of DNA hydrolase and 35 U / mL Soak in the α-galactosidase enzyme solution for 2 hours, and finally wash 7 times with purified water;

[0047] Step 3: Composite hyaluronic acid: cut the SIS processed in step 2 to 5×5 cm, soak it in a hyaluronic acid solution with a concentration of 10 mg / mL for 1 hour, take it out and vacuum freeze-dry to obtain a compounded hyaluronic acid SIS diaphragm;

[0048] Step 4. Sterilization treatment: packaging the SIS ...

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Abstract

The invention provides an anti-adhesion biological membrane and a preparation method thereof. The anti-adhesion biological membrane is a dry membrane which is formed by SIS (styrene isoprene styrene block copolymer) as a support material, compound hyaluronic acid, functional proteins and anti-inflammotary medicines. The anti-adhesion biological membrane is semi-transparent from light white to faint yellow. The thickness of the anti-adhesion biological membrane is 0.01-0.5mm. Compared with the prior art, the anti-adhesion biological membrane has very good biocompatibility, good physical barrier anti-adhesion effect and the effects of resisting inflammatory, stopping bleeding and promoting fiber degradation. The effects of the anti-adhesion biological membrane are obviously superior to those of existing anti-adhesion biological membrane products. Animal experiments prove that the effective rate of the anti-adhesion biological membrane prepared by the method for preventing the adhesion after abdominal surgery reaches above 90%, the effective rate for preventing the adhesion after pelvic cavity surgery reaches above 80%, and the effective rate for preventing the adhesion after tendon surgery reaches above 70%.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering medical biomaterials, and in particular relates to an anti-adhesion biofilm and a preparation method thereof. Background technique [0002] Postoperative adhesion is a common surgical complication. Usually more than 90% of patients will have adhesions after surgical tendon surgery, and almost 70% of patients will have varying degrees of adhesion complications after abdominal and pelvic surgery. It may lead to the loss of normal physiological function after operation, cause serious adverse symptoms such as intestinal obstruction, chronic abdominal pain, chronic pelvic pain and infertility, and even endanger life. [0003] There are many materials currently used in anti-adhesion research, such as polylactic acid, hyaluronic acid, cellulose, chitosan, silica gel, resin and collagen extracted from tendon, etc. It is expected that they can provide a temporary physical barrier for tissue heal...

Claims

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Application Information

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IPC IPC(8): A61L31/04A61L31/00A61L31/16
Inventor 陈岚刘博武
Owner XIAN TISSUE ENG & REGENERATIVE MEDICINE RES INST
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