Method for identifying purity of corn seeds
A purity identification and seed technology, applied in measuring devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of insufficient film toughness and hardness, long time-consuming protein extraction, and cumbersome gel-making procedures. The effect of easy test operation and observation, and simple process flow
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[0037] 1. Solution preparation
[0038] (1) Electrode buffer
[0039] Weigh 6.00g of glycine, pour it into a 2000mL beaker, add 1800mL of deionized water to dissolve, adjust the pH to 3.3 with 2.0ml of lactic acid, add deionized water to 2000mL, and mix well.
[0040] (2) Sample extract
[0041] Weigh 5.80g of sodium chloride, 200.00g of sucrose, and 0.1515g of methyl green AR-0721 (molecular weight: 608.75), pour them into a 1000mL beaker, and add 800ml of deionized water. Dissolve, heat to a slight boil, let it cool to room temperature, and then dilute to 1000mL with deionized water. Store at 4°C.
[0042] (3) Separating gel solution
[0043] Take 1.43mL of sodium lactate and add 980mL of deionized water, then add 129.38g of acrylic acid amine, 4.31g of N,N'-methylenebisacrylamide, 0.25g of ascorbic acid, and 8.0mg of ferrous sulfate. After dissolving, adjust the pH to 3. 0, add deionized water to make up to 1000mL, filter into a brown bottle, and store at 4°C.
[0044...
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