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Bacillus cereus (strain B2), liquid preparation, preparation method of liquid preparation, and application of B2 strain or liquid preparation to prevention and treatment of melanconium juglandinum kunze

A technology of bacillus cereus and liquid preparations, applied in the fields of bacillus cereus B2 strains, liquid preparations and their production, and in the prevention and control of walnut branch blight, which can solve the problems of phytotoxicity and drug resistance

Inactive Publication Date: 2013-11-20
SICHUAN AGRI UNIV
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Problems solved by technology

Although chemical control is an effective control method, there are many practical problems such as pathogen resistance, environment and food safety, and drug damage. Some chemical fungicides have been strictly restricted in many developed countries and regions.

Method used

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  • Bacillus cereus (strain B2), liquid preparation, preparation method of liquid preparation, and application of B2 strain or liquid preparation to prevention and treatment of melanconium juglandinum kunze
  • Bacillus cereus (strain B2), liquid preparation, preparation method of liquid preparation, and application of B2 strain or liquid preparation to prevention and treatment of melanconium juglandinum kunze
  • Bacillus cereus (strain B2), liquid preparation, preparation method of liquid preparation, and application of B2 strain or liquid preparation to prevention and treatment of melanconium juglandinum kunze

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Embodiment Construction

[0011] The present invention will be described in detail below in conjunction with specific embodiments.

[0012] (1) Screening of Bacillus cereus B2 strain

[0013] 1.1 Pathogens tested

[0014] The pathogenic bacteria tested was Melanconium juglandinum Kunze.

[0015] 1.2 Isolation and purification of spores

[0016] Take the hybrid bamboo rhizosphere soil (below the surface layer 10cm) and put it into a sterile sample bag and bring it back to the laboratory for future use. Weigh 10 g of each sample and transfer it to a conical flask containing 90 mL of normal saline and a small amount of glass beads, mix thoroughly to make a soil suspension, and heat it in a water bath at 100°C for 5 minutes. After cooling, take 1mL under aseptic conditions and add it to a test tube filled with 9mL sterile water, and mix well to obtain 10 -2 The sample diluents were diluted sequentially according to this method to obtain 10 -3 、10 -4 、10 -5 and 10 -6 Soil dilution solution, take 10 ...

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Abstract

The invention discloses bacillus cereus (strain B2), a liquid preparation, a preparation method of the liquid preparation, and application of the strain B2 or the liquid preparation to prevention and treatment of melanconium juglandinum kunze. The strain is separated from hybridized bamboo roots at Tianquan county, Ya'an city, Sichuan province in 14th June, 2010, is identified as Bacillus cereus according to form, physiology and molecules, and is preserved at CGMCC (China General Microbiological Culture Collection Center) in Chaoyang district of Beijing, China in 20th June, 2013, the preservation number is CGMCC NO:7767, and the strain is classified and named as Bacillus cereus. The strain B2 not only inhibits the colonization and the growth of pathogenic bacteria on the cuticular layer part of host limbs, but also well inhibits the breeding and the diffusion of the pathogenic bacteria in the cuticular layer of the host.

Description

technical field [0001] The invention relates to a Bacillus cereus B2 strain, a liquid preparation, a preparation method thereof and an application in preventing and treating walnut branch blight. Background technique [0002] Walnut branch blight (Melanconium juglandinum Kunze.) mainly damages branches. It mostly occurs on 1-2 year old branches, generally occurs in some areas, and the rate of diseased branches can be as high as 20% to 30%. A large number of branches are caused to die, which has a great impact on tree development and walnut yield. The cortex of the damaged branches turns dark grayish brown, and then gradually turns light reddish brown or gray, and the diseased part of the big branches is sunken. Scattered conidia discs under the cork layer of dead branches, after maturity, a large number of spores break through the cork layer from the center of the disc, and black spots (conidia discs) the size of rice grains are scattered on the diseased branches, with a d...

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/085
Inventor 朱天辉李姝江邵宝林张静韩珊谯天敏张丽娜郑磊
Owner SICHUAN AGRI UNIV
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