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Yeast and method for producing chromium-enriched yeast by utilizing yeast via high-density fermentation

A high-density fermentation and chromium-enriched yeast technology, which is applied in the production of chromium-rich yeast and yeast, can solve the problems of inability to achieve high-density fermentation and low production efficiency, and achieve the effects of increasing content, increasing production efficiency, and improving product quality

Active Publication Date: 2015-03-04
ZHEJIANG SENYO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The production efficiency of the fermentation method adopted by the existing fermentation method is low, and high-density fermentation cannot be achieved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for producing chromium-rich yeast by high-density fermentation of yeast, comprising the following steps:

[0036] (1) Inoculate the above-mentioned yeast with the preservation number CCTCC M 2013238 on the medium for high-density fermentation (the medium has been sterilized), and the components of the medium are carbon source, nitrogen source, inorganic salt and a small amount of trace elements. Glucose accounts for 400 parts by weight, yeast extract accounts for 50 parts by weight, magnesium sulfate accounts for 8 parts, and diammonium hydrogen phosphate accounts for 7 parts. The culture temperature is 30-32°C, and the pH value is 5.0-5.5.

[0037] (2) When the ethanol drops, start to add nutrients, such as glucose. In the early stage of sugar supplementation, the dissolved oxygen should be maintained and the ethanol should be kept to control the sugar supplement. In the later stage, the dissolved oxygen drops to the lowest point, about 35% (in the air The ox...

Embodiment 2

[0042] A method for producing chromium-rich yeast by high-density fermentation of the above-mentioned yeast, comprising the following steps:

[0043] (1) Inoculate the above-mentioned yeast with the preservation number CCTCC M 2013238 on the medium for fermentation (the medium has been sterilized). The medium components include carbon source, nitrogen source, inorganic salt and a small amount of trace elements. Parts by weight, corn steep liquor dry powder accounts for 60 parts by weight, magnesium sulfate accounts for 6 parts, diammonium hydrogen phosphate accounts for 3 parts and a small amount of trace elements copper, iron, zinc, manganese and cobalt. The culture temperature is 30-32°C, and the pH value is 5.0-5.5.

[0044] (2) When ethanol drops, start to add nutrients, such as glucose. In the early stage of sugar supplementation, it is necessary to maintain the decline of dissolved oxygen and maintain the decline of ethanol to control sugar supplementation. In the later ...

Embodiment 3

[0049] A method for producing chromium-rich yeast by high-density fermentation of the above-mentioned yeast, comprising the following steps:

[0050] (1) Inoculate the yeast with the above-mentioned preservation number of CCTCC M 2013238 on the medium for fermentation (the medium has been sterilized), and the medium components: 450 parts by weight of sucrose, 55 parts by weight of peptone, and 7 parts by weight of magnesium sulfate , diammonium hydrogen phosphate accounts for 5 parts and a small amount of trace elements copper, iron, zinc, manganese and cobalt. The culture temperature is 30-32°C, and the pH value is 5.0-5.5.

[0051] (2) When ethanol drops, start to add nutrients, such as glucose. In the early stage of sugar supplementation, it is necessary to maintain the decline of dissolved oxygen and maintain the decline of ethanol to control sugar supplementation. The oxygen content is 100%, with the oxygen content in the saturated sodium sulfite solution being 0% as a r...

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Abstract

The invention discloses a yeast and a method for producing a chromium-enriched yeast by utilizing the yeast via high-density fermentation, wherein the collection number of the yeast is CCTCC No. M2013238. The method for producing the chromium-enriched yeast comprises the following steps of: (1) inoculating the yeast with the collection number of CCTCC No. M2013238 on a culture medium, and performing high-density fermentation; (2) performing feedback feeding on nutrient substances by virtue of the combination of ethyl alcohol and dissolved oxygen; (3) controlling the concentration of inorganic chromium in a fermentation system at 50-200 mg / L by feeding inorganic chromium; (4) stopping fermentation after the yeast stops growth, and centrifuging, washing and drying to obtain the chromium-enriched yeast. By virtue of the yeast and the method disclosed by the invention, conversion for inorganic chromium can be much effectively realized, and the content of organic chromium can achieve 6500 ppm. Via the feedback feeding technology, high-density fermentation for the chromium-enriched yeast is realized, and the yield of wet yeasts is 250-320 g / L, thus effectively increasing production efficiency.

Description

technical field [0001] The invention relates to fermentation technology, in particular to a yeast and a method for producing chromium-rich yeast by high-density fermentation of the yeast. Background technique [0002] Chromium is one of the essential micronutrients for animals and humans. The best sources of chromium are meat, especially liver and other offal, which are sources of bioavailable chromium. Brewer's yeast, unprocessed grains, bran, nuts, and cheese also provide more chromium. Therefore, obtaining chromium through yeast or yeast products is one way to supplement chromium. [0003] For chromium-rich yeast, there has been some research in the prior art. The Chinese patent "A chromium-rich Saccharomyces cerevisiae, a chromium-rich yeast product and its production method (Patent No.: 200610066365.X)" discloses the use of lithium chloride for mutagenesis , the chromium-enriched Saccharomyces cerevisiae bred on the chromium-containing medium, the chromium-enriched y...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18C12R1/865
Inventor 茅惠忠何成保宋明根
Owner ZHEJIANG SENYO BIOTECH
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