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A kind of aseptic seeding and test-tube seedling propagation method of Jianlan

A technology for aseptic sowing and test-tube seedlings, applied in the field of plant reproduction, can solve the problems of inability to achieve industrial development, unable to meet the needs of fine varieties, limited number of seedlings, etc., and achieve high germination rate, fast germination, and seedlings. good quality effect

Active Publication Date: 2014-10-15
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for many excellent varieties with leaf art and flower art, due to the limited number of seedlings, it is impossible to achieve industrial development
Unable to meet the market demand for fine varieties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Jianlan golden silk horsetail selfing and seed aseptic sowing and test-tube plantlet propagation method

[0027] 1. Material selection: In mid-to-early June, Jianlan golden horsetail blooms, and the healthy and healthy golden horsetail is selected as the parent. After 2 days of flowering, artificial self-pollination is carried out. Before and after pollination, the whole flower stems are covered with pollination bags. The pedicel selects 2 flowers for pollination, adopts cross-pollination of the same plant, removes the labial petals of the parents after pollination, and removes the remaining flowers that are not pollinated on the pedicel. After 15 days, the pollination bag was removed to check the ovary expansion, and the result rate was 100%.

[0028]2. Sterile sowing and seed pretreatment: 150 days after pollination, the capsules are yellow-green, and the hybrid capsules are 7-8 minutes mature. Cut off the capsules, first clean the surface with 75% alcoh...

Embodiment 2

[0035] Embodiment 2: the hybridization of Jianlan golden wrinkled rainbow lotus and Qingshan Yuquan and seed aseptic sowing and test-tube seedling propagation method

[0036] 1. Material selection: In mid-to-early July, when the two parents bloom at the same time, 5 days after flowering, they are the parents of each other and carry out reciprocal crossing. Before and after pollination, the whole flower stems are covered with pollination bags, and 2 flowers are selected for each flower stem to pollinate. After pollination, the lip petals of the female parent are removed, and the remaining flowers that are not pollinated on the flower stems are removed. Remove the pollination bag after 15 days, check the ovary expansion situation, the result rate is 100%.

[0037] 2. Sterile sowing and seed pretreatment: 180 days after pollination, the hybrid capsules are 7-8 minutes mature, and the capsules are yellow-green. Cut off the capsules, first clean the surface with 75% alcohol by volu...

Embodiment 3

[0044] Embodiment 3: the hybridization of Jianlan Yipinmei and Fulong, aseptic sowing of seeds and propagation method of test-tube plantlets

[0045] 1. Material selection: In the middle and late July, 7 days after the first flower of Jianlan Yipinmei, Fulong began to bloom, and 10 days after the first flower of Jianlan Yipinmei, the two parents were used as parents for each other. Reciprocal. Before and after pollination, the whole flower stems are covered with pollination bags, and 2 flowers are selected for each flower stem to pollinate. After pollination, the lip petals of the female parent are removed, and the remaining flowers that are not pollinated on the flower stems are removed. Remove the pollination bag after 15 days, check the ovary expansion situation, the result rate is 100%.

[0046]2. Sterile sowing and seed pretreatment: 180 days after pollination, the capsules are fully developed, yellow-green, and the hybrid capsules are mature at 7-8 minutes. Cut off the ...

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Abstract

The invention discloses a sterile seeding and test-tube plantlet propagation method of sword-leaved cymbidiums. The aim of the sterile seeding and test-tube plantlet propagation method is realized by key technologies of selecting parents to pollinate, carrying out seed sterile seeding pre-treatment to increase the germination rate, increasing the reproduction rate of root stocks, increasing the differentiation rate of the root stocks, accelerating the growth of seedlings and increasing the rooting rate of the seedlings, increasing the transplanting survival rate of the seedlings and the like. According to the sterile seeding and test-tube plantlet propagation method disclosed by the invention, the seed germination speed is fast, the germination rate is high, the number of buds differentiated by the root stocks is more, bud rooting is good and the quality of the seedlings is good; a lot of test-tube seedlings can be obtained rapidly; the transplanting survival rate of grown-up seedlings is up to more than 90%, so that the method provides an effective way for producing the seedlings of the sword-leaved cymbidiums.

Description

Technical field: [0001] The invention belongs to the field of plant propagation, and in particular relates to a method for aseptic sowing and test-tube seedling propagation of Jianlan. Background technique: [0002] Guolan is a traditional inscription flower in my country, with high ornamental value and economic value. It usually refers to a part of the ground species in the Orchid family, generally including Jianlan (Cymbidium ensifolium), Chunlan (C.goeringii), Cymbidium (C. .faberi), ink orchid (C.sinense), cold orchid (C.kanran), lotus petal orchid (C.tortisepalum), and spring sword 7 species. The orchid is elegant and fragrant, known as the "gentleman among flowers", and is highly appreciated by literati. It has been cultivated in my country for more than 2,000 years and has formed a unique orchid culture. The orchid is also very popular in Southeast Asian countries. [0003] Although orchid has been popular in my country for thousands of years, its development and util...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 张建霞段俊吴坤林曾宋君
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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