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Proteus mirabilis capable of eliminating ammonia nitrogen in water environment and application thereof

A technology of Proteus mirabilis and water environment, applied in the field of Proteus mirabilis

Active Publication Date: 2013-10-30
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the elimination of ammonia nitrogen in the environment by Proteus mirabilis

Method used

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  • Proteus mirabilis capable of eliminating ammonia nitrogen in water environment and application thereof
  • Proteus mirabilis capable of eliminating ammonia nitrogen in water environment and application thereof
  • Proteus mirabilis capable of eliminating ammonia nitrogen in water environment and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Screening of Proteus mirabilis capable of reducing the concentration of ammonia nitrogen in the environment

[0018] Inoculate the Proteus mirabilis seeds stored at -80°C in LB medium, the liquid volume of the shake flask is 30%, the culture temperature is 27-30°C, the culture speed is 150-200rpm / min, and the culture time is 10-16 hours.

[0019] Collect the above-mentioned thalli, and divide the collected Proteus mirabilis V7 into 1.0×10 7 CFU / ml of Proteus mirabilis was inoculated in ammonia nitrogen medium for culture, and the ammonia nitrogen medium without bacteria was used as a control. At different time points, 1ml of the culture solution was taken out, centrifuged at 13,000rpm for 5min, and the supernatant was reserved. The supernatant was semi-quantitatively determined the ammonia nitrogen concentration using the ammonia nitrogen semi-quantitative determination kit (Hangzhou Luheng Biotechnology Co., Ltd.). The yellower the color, the lower the ammo...

Embodiment 2

[0020] Example 2: Changes in ammonia nitrogen content over time after inoculation of Proteus mirabilis V7

[0021] Dilute Proteus mirabilis V7 by 1.0 × 10 7 The initial concentration of CFU / mL was inoculated into the ammonia nitrogen medium, and then loaded into the shake flask, the liquid volume was 30% of the volume of the shake flask, the culture temperature was 28-30°C, and the culture speed was 150-200rpm / min. Take out 1ml of the bacterial culture solution every 24 hours, centrifuge at 12,000rpm for 5min, and analyze the content of ammonia nitrogen in the supernatant by Nessler's reagent colorimetry. See image 3 . After 4 days of inoculation, the removal efficiency of ammonia nitrogen was relatively stable and could reach more than 85%.

Embodiment 3

[0022] Example 3: Effects of Different Initial Concentrations of Ammonia Nitrogen on Ammonia Nitrogen Scavenging Efficiency

[0023] Proteus mirabilis V7 was inoculated into ammonia nitrogen medium containing 20, 50, 100, 200, 500, 1000 and 2000 mg / L, respectively (the initial concentration of bacteria at each concentration was 1.0×10 7 CFU / mL), and then loaded in the shake flask, the volume of the liquid was 30% of the volume of the shake flask, the culture temperature was 28-30°C, and the culture speed was 150-200rpm / min. After 64 hours, 1 ml of bacterial culture solution was taken out, centrifuged at 12,000 rpm for 5 minutes, and the content of ammonia nitrogen in the supernatant of the culture solution was analyzed by Nessler's reagent colorimetric method. The change process of ammonia nitrogen removal efficiency with time after inoculation with Proteus mirabilis V7 is shown in image 3 . When the ammonia nitrogen concentration was 20, 50 and 100mg / L, the efficiency of P...

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Abstract

The invention discloses a proteus mirabilis strain V7 capable of eliminating ammonia nitrogen in water environment. The strain is preserved in the Chinese ordinary microorganism culture preservation and administration center of microbiology institute of Chinese Academy of Sciences on November 06th, 2010, and the preservation number is CGMCC No. 4312. The strain is capable of rapidly and efficiently lowering the concentration of ammonia nitrogen in low-salt and high-salt water environment, and ammonia monooxygenase (amoA) genes of the proteus mirabilis participate in the process that ammonia nitrogen is degraded by the proteus mirabilis.

Description

technical field [0001] The invention relates to the field of environmental treatment by using microorganisms, in particular to Proteus mirabilis capable of removing ammonia nitrogen in water environment and its application. Background technique [0002] With the continuous development of the aquaculture industry, the degree of intensification continues to increase, and the pollution of aquaculture water is intensified. Residual bait, feces, and antibiotics used in the breeding process are the main sources of water pollution in aquaculture, which can cause eutrophication of the water body and C, N, and P enrichment pollution of the sediment. Among them, ammonia nitrogen is one of the important pollutants. Ammonia nitrogen, as a nutrient in water bodies, can lead to water eutrophication. It is the main oxygen-consuming pollutant in water bodies and is poisonous to fish and some aquatic organisms. Ammonia nitrogen of 2-10mg / L can reach the lethal dose to aquaculture organisms...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 张卫卫陈令新王巧宁
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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