Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tissue culture and rapid propagation method of nobile-type dendrobium seedlings

A tissue culture and dendrobium orchid technology, applied in the field of plant tissue culture, can solve the problems of large differences between spring dendrobium species, high requirements on operation technology, easy contamination of explants, etc. Overcome the effects of low reproduction coefficients

Inactive Publication Date: 2013-10-30
SHANGHAI ACAD OF AGRI SCI
View PDF5 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, due to the easy pollution of explants, high operating technical requirements, low reproduction rate, high cost, and large differences among the species of Dendrobium nodulum, there has been no report on large-scale production of Dendrobium orchid seedlings by tissue culture technology.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue culture and rapid propagation method of nobile-type dendrobium seedlings
  • Tissue culture and rapid propagation method of nobile-type dendrobium seedlings
  • Tissue culture and rapid propagation method of nobile-type dendrobium seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1) Preparation and disinfection of explants: artificially pollinate Dendrobium orchids and bag them, and pick the capsules about 100 days later. On the ultra-clean workbench, disinfect the surface of the capsule with 75% (volume ratio) alcohol for 30-60 seconds, then soak it in 15-20% (volume ratio) sodium hypochlorite solution for 20 minutes, and rinse it with sterile water for 3 times.

[0032] 2) Induction culture of protocorms: dry the surface moisture of the sterilized Dendrobium capsules with sterile absorbent paper, cut the capsules with a scalpel, pick Dendrobium seeds, and inoculate them on the induction medium for dark culture ( The formula of induction culture medium is: MS+BA 0.6mg / L+NAA 0.1mg / L+hydrolyzed casein 0.5g / L+banana puree 40g / L+sucrose 30g / L+agar powder 6.0g / L, pH=5.4~5.6); After 20-30 days, the seeds of Dendrobium orchids began to germinate, and the culture was transferred to the light to continue to cultivate. After 40-50 days of light culture, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a tissue culture and rapid propagation method of nobile-type dendrobium seedlings. The method comprises the following steps of: (1) selection and disinfection of an explant; (2) induction culture of protocorm; (3) proliferation and differentiation of protocorm; (4) strong seedling and rooting culture; (5) transplanting of test-tube plantlets. According to the method, tissue culture and rapid propagation of the nobile-type dendrobium seedlings are carried out by the method provided by the invention, the culture procedure is simple, the explant is free of pollution, the propagation coefficient is large, the production cost is low, the seedlings are high in quality, and the defects of low propagation coefficient and the like in the prior art are overcome.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a tissue culture rapid propagation method for spring dendrobium orchid seedlings. Background technique [0002] Dendrobium (Dendrobium) is a perennial flower of the Orchidaceae Dendrobium genus. Dendrobium is the largest genus in the Orchidaceae. Its origin is mainly distributed in tropical and subtropical Asia, Australia and Pacific Islands. There are more than 1,000 species in the world. There are about 76 species in my country, most of which are distributed in Southwest China, South China, Taiwan and other places. [0003] In horticulture, the varieties of Dendrobium orchids are generally divided into two categories: spring Dendrobium and autumn Dendrobium by their flowering period. Spring Dendrobium nobile (Dendrobium nobile) is a deciduous temperate species. Its flowers are brightly colored, luxurious and splendid, and are often planted as potted flow...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
Inventor 殷丽青汤桂钧周音李秀芬黄卫昌胡永红将建平刘炤胡海锋范晓芬
Owner SHANGHAI ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com