A kind of laser Raman detection method of malachite green
A malachite green and detection method technology, applied in the field of analysis and detection, can solve problems such as abnormal detection, complicated detection process, difficult and accurate judgment, etc., and achieve the effects of convenient product quality, food safety assurance, and practical detection technology
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Embodiment 1
[0043] Prepare a 100μg / mL malachite green standard aqueous solution; prepare an enhanced chip: drop the nano-silver colloidal aqueous solution onto the aluminum foil, and after the addition is complete, the silver glue has a diameter of 3mm and dried to obtain an enhanced chip; drop 10μL of the malachite green standard aqueous solution Add it to the enhanced chip and dry to obtain the malachite green standard sample; use the American Ocean Optics RAM-PRO-785E portable laser Raman spectrometer to detect the malachite green standard sample to obtain the malachite green standard Raman spectrum. The main characteristic peak wave number is: 1176cm -1 ,1397.7cm -1 , And 1618.6cm -1 (See figure 2 ). Use the American Ocean Optics RAM-PINPNTR-785 handheld laser Raman spectrometer to scan the standard samples of malachite green (Raman spectrum scanning is performed to obtain the characteristic peak map of malachite green, the main characteristic peak wave number is: 1176cm -1 ,1397.7cm -...
Embodiment 2
[0045] Prepare a 0.2μg / mL malachite green aqueous solution; take 10μL dots on the silver gel enhanced chip, dry, and use the American Ocean Optics RAM-PRO-785E portable laser Raman spectrometer to detect the malachite green standard sample points to obtain malachite Green laser Raman spectrum (see Figure 4 ).
Embodiment 3
[0047] Detection of malachite green in fish.
[0048] Preparation of fish meat containing malachite green: add 5 g of crushed fish meat to a 50 mL centrifuge tube, and then add 0.5 mL of 2 ppm malachite green aqueous solution to the centrifuge tube, and stir with a glass rod for 15 minutes to fully absorb the mixed malachite green.
[0049] Extraction: Add 10 mL of acetonitrile to the above 50 mL centrifuge tube and homogenize for 30 seconds at 10000 r / min. Add 5 g of acidic alumina and shake for 2 minutes, centrifuge at 4000 r / min for 10 minutes, transfer the supernatant to a 125 mL separatory funnel, add 2 mL of diethylene glycol to the separatory funnel, and shake for 2 minutes.
[0050] Take a 50mL centrifuge tube and add 10mL acetonitrile, wash the homogenizer blade for 10 seconds, pour the washing solution into the previous centrifuge tube, use a glass rod to disperse the sediment in the centrifuge tube and stir evenly, vortex the mixer for 1 min, and let it stand for 20 min. ...
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