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Degradable compound active amnion material, preparation method and application thereof and compound active amnion uterine cavity repair stent

An amniotic and active technology, which is applied in the field of composite active amniotic membrane uterine cavity repair, can solve the problems of accelerated postoperative adhesion, secondary damage of metal stents, and poor effect, and achieves strong operability, avoids secondary damage, and protects the human body. good adaptability

Active Publication Date: 2014-12-24
JIANGXI RUIJI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a degradable composite active amniotic uterine cavity repair bracket, so as to solve the problem of inflammation caused by the inability to effectively separate the front and rear walls of the uterus in the prior art, thereby accelerating postoperative adhesion; the removal of the metal bracket will cause secondary damage to the human body ; The effect of drug treatment on patients with severe IUA is not optimistic, and the postoperative re-adhesion rate is over 50%; it does not have the function of promoting endometrial regeneration and repair;

Method used

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  • Degradable compound active amnion material, preparation method and application thereof and compound active amnion uterine cavity repair stent
  • Degradable compound active amnion material, preparation method and application thereof and compound active amnion uterine cavity repair stent
  • Degradable compound active amnion material, preparation method and application thereof and compound active amnion uterine cavity repair stent

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preparation example Construction

[0030] 1. Preparation of composite active amniotic membrane material

[0031] 1.1 Preparation of amniotic membrane

[0032] The amniotic membrane in this embodiment adopts human amniotic membrane, and the human amniotic membrane can be selected from commercial human amniotic membranes that meet the standard (YZB / Guo 0593-2005). During the treatment of the amniotic membrane, the amniotic membrane epithelial cells can be retained or the epithelial cells can be removed by enzymatic hydrolysis , no matter whether the epithelial cells are removed or not, the preparation of the composite active amniotic membrane material in the present invention is not affected. Among them, the methods are as follows:

Embodiment 1

[0034] Preparation of amniotic membrane retaining epithelial cells: In a clean and sterile environment, fresh amniotic membranes were rinsed with sterile saline, with the epithelial layer facing upward, and were flattened on 0.45um microporous nitrocellulose filter paper, placed in 0.2% ~ Soak in 2% glycerol for later use.

Embodiment 2

[0036] Preparation of amniotic membrane without epithelial cells: in a clean and sterile environment, fresh amniotic membrane is rinsed with sterile water, and then treated with 0.2% to 0.5% trypsin or 0.25% trypsin + 0.02% EDTA (ethylenediaminetetraacetic acid, Ethylene diamine tetraacetic acid), hydrolyzed by shaking in a water bath at 37°C for 20 to 120 minutes, remove epithelial cells, fibroblasts and serum with a cell scraper, and rinse with sterile water repeatedly. At this time, the decellularized amniotic membrane is a translucent membrane, HE Staining (hematoxylin-eosin staining), no epithelial cell residues were observed under light microscope, and soaked in 0.2%-1% glycerol for later use.

[0037] 1.2 Preparation of collagen sponge or composite collagen sponge preparation solution

[0038] The present invention includes two preparation methods of collagen sponges. The collagen sponges or composite collagen sponges prepared by the following methods are suitable for t...

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Abstract

The invention provides a compound active amnion material, a preparation method and application thereof and a compound active amnion uterine cavity repair stent. The preparation method comprises the following steps: (1) providing a collagen sponge prepare liquid or a compound collagen sponge prepare liquid; (2) providing an amnion with both an epithelial layer and a basement membrane; (3) pouring and coating the prepared prepare liquid on the basement membrane of the amnion to enable the basement membrane and the prepare liquid to be subject to crosslinking, and then preparing the compound active amnion material through freeze drying. The compound active amnion material not only keeps the complete structure and the due biological function of the amnion but also endow the amnion with a certain rigidity, so that the compound active amnion material has stronger maneuverability in application after TCRA (Transcervical Resection Of Adhesions) operation; the compound active amnion uterine cavity repair stent integrates the compound active amnion material and a saccule at the front end of a pipe body, the saccule expands under the action of an external force to push out the compound active amnion material, and then the compound active amnion material is successfully attached to the inner wall of the uterine cavity; the stent is convenient to take out and prevents secondary damage.

Description

technical field [0001] The invention relates to the technical field of gynecological intrauterine adhesion treatment, more particularly to a degradable composite active amniotic membrane material, a preparation method and application thereof, and a composite active amniotic membrane uterine cavity repair stent. Background technique [0002] Intrauterine adhesions (IUA), also known as Asherman syndrome, refers to the mutual adhesion of the uterine muscle wall and / or the cervical canal after damage to the uterine cavity or the endometrium of the cervical canal due to various factors. Affect menstruation and reproductive function in women of childbearing age. Trauma, infection, uterine malformation and genetic predisposition to the uterine cavity during pregnancy and non-pregnancy are the main reasons for the formation of intrauterine adhesions. The main histopathological changes of intrauterine adhesions are endometrial fibrosis and scarring. Clinically, intrauterine adhesion...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071A61L31/12A61M31/00
Inventor 傅筱冲熊贞燕滕志强丁科林苗春云
Owner JIANGXI RUIJI BIOTECH CO LTD
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