In-vitro tissue culture and rapid propagation method of Terminalia mantaly Tricolor

Abstract

The invention provides a Terminalia mantaly Tricolor tissue culture and propagation method. The Terminalia mantaly Tricolor tissue culture and propagation method comprises the following steps of: carrying out tissue culture and rapid propagation under different hormone ingredients and concentration levels by using MS as basic culture medium, adding a natural organic mixture, and using the leaves or twigs of the Terminalia mantaly Tricolor as explants; carrying out disinfection treatment and inoculation induction to form a callus tissue; carrying out callus tissue differentiation, bud multiplication and rooting culture to form a complete plant seedling; transplanting the complete seedling in a raising substrate for growing into a normal Terminalia mantaly Tricolor plant, wherein the basic culture medium is based on MS culture media and supplemented with the ingredients including 6-benzylaminoadenine, 2,4-dichlorphenoxyacetic acid, naphthylacetic acid, sucrose, active carbon and the like. According to the in-vitro issue culture and rapid propagation method of Terminalia mantaly Tricolor disclosed by the invention, the tissue culture is applied to overcome the problems that the Terminalia mantaly Tricolor is long in seedling raising period and low in propagation coefficient, so that rapid industrialized seedling can be carried out for meeting the requirements of the market on the Terminalia mantaly Tricolor seedling.

Description

technical field [0001] The invention relates to a method for in vitro tissue culture and rapid propagation of Terminalia jinye. Background technique [0002] Terminalia jinye is a cultivated variety of Terminalia microphylla, the plant height can reach 10m, and the side branches grow in whorls and spread out horizontally. The main trunk is straight, the tree is beautiful, and the layers are distinct. The greening and beautifying effects are very good, and it is suitable for garden greening. In recent years, the side branches are distributed horizontally in layers, and the light green leaves have milky white or milky yellow spots, which are very popular in greening projects in South China. Five or six years ago, all the plants used in the market came from Taiwan, so the price was relatively expensive. Each plant with a DBH of 5 to 6 cm cost 3,000 yuan. Around 2000, some nurseries in South China began to use Terminalia lobata with a height of 1 meter as rootstock for graftin...

AI Technical Summary

Problems solved by technology

However, after many years of observation, no flowering and fruiting of this tree species have been found, so the sexual reproduction of this tree species is limited. At present, only asexual reproduction is carried out. There are three ways of asexual reproduction, one is cutting, one is grafting, and the other is It is in vitro tissue culture and rapid propagation. Through investigation, it is found that the seedlings of Terminalia jinye are generally produced by grafting. The method is primitive and time-consuming and labor-intensive. At the same time, it is affected by weather conditions and cannot produce seedlings on a large scale.