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Test paper for detecting fluoroquinolone drugs and its application

A fluoroquinolone and drug technology, applied in the field of immunochemistry, can solve the problems of high degree of instrumentation, complex sample processing, technical difficulty, etc., and achieve the effects of high sensitivity, improved detection efficiency, and improved sensitivity

Active Publication Date: 2013-09-25
BEIJING KWINBON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For cosmetics, the matrix is ​​complex and diverse, and the technology is difficult. Although the instrument detection method has the advantages of high detection accuracy, it is not suitable for the detection of a large number of samples due to its high degree of instrumentation, complicated sample processing, and high detection costs. The immunological detection method is simple, fast and sensitive, and can detect most samples at the same time. It is an ideal rapid screening method

Method used

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  • Test paper for detecting fluoroquinolone drugs and its application
  • Test paper for detecting fluoroquinolone drugs and its application
  • Test paper for detecting fluoroquinolone drugs and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation of embodiment one fluoroquinolones test paper

[0046] 1. Preparation of Fluoroquinolone Haptens

[0047] (1) Dissolve 1 to 3 mmol of norfloxacin in 30 to 60 ml of chloroform, in the dehydrating agent 1,3-dicyclohexylcarbodiimide (DCC) and catalyst 4-dimethylaminopyridine (DMAP) Under the action of 1-3mmol of tert-butyl aminopropionate, stir at room temperature for 1-5 hours to obtain the condensate of norfloxacin and ethyl p-aminophenylacetate, wash the obtained condensate with water, dry it, and pass through silica gel Purified by column chromatography, and the eluent was ethyl acetate-petroleum ether.

[0048] (2) Dissolve the purified norfloxacin and ethyl p-aminobenzyl acetate condensate in methanol, add NaOH, stir at 50-70°C for 0.5-2 hours, carry out ester hydrolysis reaction, and remove the solvent under reduced pressure to obtain The crude fluoroquinolone hapten is p-carboxymethylbenzamide norfloxacin, then dissolve p-carboxymethylbenzamide no...

Embodiment 2

[0086] The detection of fluoroquinolone residues in the sample of embodiment two

[0087] 1. Sample pretreatment

[0088] Creamy, milky cosmetics or lotion or: Weigh 0.1g of creamy or lotiony cosmetics or 0.1ml of lotion into a centrifuge tube, add 5mL of 0.02mol / L phosphate buffer solution, vortex the sample until uniform, pending inspection.

[0089] 2. Detection with test paper

[0090] Use a straw to absorb the sample solution to be tested and drop 2 to 3 drops vertically into the sample hole, react for 5 to 10 minutes, and judge the result. 3. Analyze test results

[0091] Negative (-): Both the T line and the C line are colored, indicating that the concentration of fluoroquinolones in the sample is lower than the detection limit, such as figure 2 (a).

[0092] Positive (+): No color on the T line and color on the C line, indicating that the concentration of fluoroquinolones in the sample is equal to or higher than the detection limit, such as figure 2 (b).

[0093...

Embodiment 3

[0094] Example three sample detection example

[0095] 1. Detection limit test

[0096] Take blank creamy or milky cosmetics or lotion samples respectively, add fluoroquinolones enrofloxacin, difloxacin, flumequine, danofloxacin, Flufloxacin, norfloxacin, sarafloxacin, ofloxacin, and ciprofloxacin were added to final concentrations of 1, 2.5, and 5 mg / kg (L), respectively, and ciprofloxacin and enoxacin were added to The final concentrations were 2.5, 5, and 10 mg / kg (L), and the test paper was taken for detection, and each sample was repeatedly measured three times.

[0097] When using test paper to detect creamy, milky cosmetics or lotion samples, when the added concentration of fluoroquinolones in the creamy, milky cosmetics or lotion samples is 3 μg / L, two red lines visible to the naked eye will appear on the test paper, showing Negative; when the fluoroquinolones enrofloxacin, difloxacin, flumequine, dafloxacin, pefloxacin, norfloxacin, sarafloxacin, ofloxacin, cyclo W...

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Abstract

The invention provides a test paper for detecting fluoroquinolone drugs. The test paper includes a sample absorption pad (1), a conjugate release pad (2) and a conjugate release pad (3), a reaction membrane (4), a water absorption pad (5) and a PVC (8) baseboard. The reaction membrane is provided with a detection line (6) coated with a fluoroquinolone-carrier protein conjugate and a quality control line (7) coated with a rabbit anti-sheep anti-antibody. The conjugate release pad (2) is coated with a sheep anti-mouse anti-antibody-colloidal gold marker, and the conjugate release pad (3) is coated with a fluoroquinolone monoclonal antibody. The invention also provides a method for applying the fluoroquinolone test paper to detection of fluoroquinolone drugs. The test paper provided by the invention can be used for detecting fluoroquinolone drug residue in cosmetics, and has the characteristics of simple operation, high sensitivity, fast detection speed and low cost, thus being suitable for screening of a large number of samples and field monitoring.

Description

technical field [0001] The invention relates to the technical field of immunochemistry, in particular to a test paper for detecting fluoroquinolones and its application. Background technique [0002] Fluoroquinolones are the most important breakthrough in the synthesis of antibacterial drugs after the rapid development of sulfa drugs in the past 20 years. The development of this type of drugs has gone through the following stages: In 1962, American scientists discovered the first fluoroquinolone Nalidixic acid, a class of antibacterial drugs, followed by Olinic acid and pyrrole acid, which became the first generation of fluoroquinolones. Due to the narrow antibacterial spectrum, short half-life, toxic side effects and bacterial resistance, etc., it is rarely used now. In the 1970s, 2nd generation fluoroquinolones such as pipemidic acid and fluoroquinolone were developed. In the 1980s, the third-generation fluoroquinolones with wider antibacterial spectrum and stronger anti...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558
Inventor 万宇平吴鹏冯静赵正苗余厚美聂雯莹田甜蒲小容
Owner BEIJING KWINBON BIOTECH
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