A kind of Bacillus s-2-6 and its antibacterial application

Abstract

The invention relates to Bacillus sp. S-2-6 CGMCC No.6477, which is obtained from the flowing steps: sampling a misgurnus anguillicaudatus sample in a misgurnus anguillicaudatus breeding farm, taking intestinal tract of the misgurnus anguillicaudatus sample, carrying out freezing grinding, filtering with gauze, taking the filtrate, carrying out gradient dilution, coating on a flat plate (TSB culture medium), culturing for 24 h at a temperature of 28 DEG C, selecting a typical single colony according to different characteristics such as colony morphology, a size, edge uniformity, transparency and the like, carrying out streaking, purifying, and storing in 15% glycerol at a temperature of -20 DEG C so as to separate the S-2-6 bacterial. The S-2-6 bacterial has effects of inhibitions of Aeromonas veronii, Aeromonas sobria and Vibrioharveyi.

Description

technical field [0001] The invention relates to a microbial strain, especially a bacillus S-2-6, and also relates to the bacteriostasis application of the bacillus S-2-6. Background technique [0002] Loach ( Misgurnus anguillicaudatus ) belongs to Cyprinidae, Loach family, has excellent nutrient composition structure, and its breeding prospect is optimistic. However, with the development of the loach farming industry, disease problems have become increasingly prominent. For example, in September 2007 and August 2012, loach farms in Donghai and Guanyun, Jiangsu, suffered from sudden diseases and suffered heavy losses. The loach diseases found so far are mainly bacterial diseases caused by infections such as Aeromonas temperatus, Vibrio vulnificus, Aeromonas loach and Aeromonas vanlon. Such bacteria are commonly found in fresh water, sea water, silt and Sewage and other environments are distributed all over the world. [0003] At present, the prevention and control of loach...

AI Technical Summary

Problems solved by technology

[0003] At present, the prevention and control of loach disease is the same as that of other aquatic diseases. Traditional methods such as chemically synthesized drugs and antibiotics are mostly used. Residues in the body, long-term intake of the human body can lead to chronic poisoning, etc., affecting the sustainable development of the aquaculture industry; the abuse of antibiotics has also repeatedly hindered the export of aquatic products in our country, causing huge losses to the aquaculture industry

As a fresh aquatic product for export, loach has a high inspection standard for drug (antibiotic) residues, and the use of antibiotics is not allowed in actual production, resulting in extremely limited means for the prevention and treatment of loach bacterial diseases

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