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Method for detecting pesticide residues in vegetables

A technology for pesticide residues and vegetables, applied in the field of pesticide residue detection in vegetables, can solve problems such as complex chemical components

Inactive Publication Date: 2013-09-18
NANNING JIANXIONG KITCHEN EQUIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many kinds of pesticides and complex chemical components, and there are many kinds of vegetables on the market, so it is necessary to provide a method that can quickly and easily detect the amount of pesticide residues in vegetables

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take four parts of Chinese cabbage sprayed with different concentrations of 10g, cut into shreds, and label D1, D2, D3, D4, put D1, D2, D3, D4 Chinese cabbage in a mixture of acetone, dichloromethane, ethyl acetate One or more of the extraction agents in the configuration, the vegetable and the extraction agent are mixed according to the solid-liquid ratio of 0.2g / ml, the ultrasonic extraction time is 4min, after the ultrasonic extraction is completed, activated carbon is added for centrifugal purification, and the upper layer is recovered after purification Extraction solution, blow off the extractant in the eluent;

[0018] S2: Add a chelating agent to the above-mentioned four parts of the extract obtained after blowing off the solvent for mixing, then add reduced glutathione and glutathione transferase to catalyze the mixed reaction, and the reduced glutathione in the mixed solution Peptide content is 5 x 10 -5 mol / L, glutathione transferase content is 0.75U / ml, aft...

Embodiment 2

[0022] Take four cucumbers sprayed with different concentrations of 10g and cut them into pieces, and label them as H1, H2, H3, and H4. One or more kinds of extraction agents, the vegetable and the extraction agent are mixed according to the solid-liquid ratio of 0.5g / ml, the ultrasonic extraction time is 4min, after the ultrasonic extraction is completed, activated carbon is added for centrifugal purification, and the upper layer extract is recovered after purification. Blow off the extractant in the eluent;

[0023] S2: Add a chelating agent to the above-mentioned four parts of the extract obtained after blowing off the solvent for mixing, then add reduced glutathione and glutathione transferase to catalyze the mixed reaction, and the reduced glutathione in the mixed solution Peptide content is 1 x 10 -5 mol / L, glutathione transferase content is 0.5U / ml, after the reaction is complete, add phosphate buffer solution with pH=0.8 to adjust the pH to 7.4 samples to be tested re...

Embodiment 3

[0027] Take four parts of water spinach sprayed with different concentrations of 10g, cut them into pieces, and mark them as K1, K2, K3, and K4. Put the cucumbers of the K1, K2, K3, and K4 groups in a mixture of acetone, dichloromethane, and ethyl acetate. One or several kinds of extraction agents, the vegetables and the extraction agent are mixed according to the solid-liquid ratio of 0.4g / ml, the ultrasonic extraction time is 4min, after the ultrasonic extraction is completed, activated carbon is added for centrifugal purification, and the upper layer is recovered after purification Liquid, blow off the extractant in the eluent;

[0028] S2: Add a chelating agent to the above-mentioned four parts of the extract obtained after blowing off the solvent for mixing, then add reduced glutathione and glutathione transferase to catalyze the mixed reaction, and the reduced glutathione in the mixed solution Peptide content is 3 x 10 -5 mol / L, glutathione transferase content is 0.65U / ...

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Abstract

The invention discloses a method for detecting pesticide residues in vegetables. The method comprises the following steps of: chopping the vegetables to be detected; adding an extracting agent for ultrasonic extraction to obtain an extracting solution, and preparing a test sample solution to be detected by using the extracting solution; and then adding flour esterase and a developing agent into the test sample solution to be detected, uniformly shaking the mixture, standing, adding a substrate solution into the mixture, then putting the mixture into a colorimetric pool for color development, and measuring absorbance variation values of the test sample solution to be detected and a contrast test sample liquid within a color development reaction time period. According to the method, organophosphorus and carbamates pesticide in the vegetables can be detected quickly; the operation is quick and convenient; furthermore, the carbamate is catalyzed by reduced glutathione and glutathione transferase, so that the inhibition effect of the carbamic acid ester against the flour esterase is improved, and the sensitivity of detection is improved; other components except the vegetable extracts of the contrast test sample solution are consistent with components in the test sample solution to be detected.

Description

technical field [0001] The invention relates to the detection field, in particular to a method for detecting pesticide residues in vegetables. Background technique [0002] Pesticides play a significant role in promoting the development of forestry and animal husbandry, but the environmental pollution and human health problems caused by the simultaneous use of pesticides are becoming more and more serious. The hazards of pesticide residues have also attracted more and more attention, and many countries have established pesticide residue limits in food. At present, there are many kinds of pesticides with complex chemical components, and there are many kinds of vegetables on the market. Therefore, it is necessary to provide a method that can quickly and easily detect the amount of pesticide residues in vegetables. Contents of the invention [0003] The object of the present invention is to provide a method for detecting pesticide residues in vegetables, which can quickly an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/48C12Q1/44G01N21/31
Inventor 李应林
Owner NANNING JIANXIONG KITCHEN EQUIP
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