Pool healthy culture micro-ecologic water purifier
A water purifier and micro-ecological technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, chemical instruments and methods, etc., can solve the problem of low survival rate of effective viable bacteria, unstable product quality, use Unsatisfactory effect and other problems, to achieve significant water purification effect, stable water purification effect, and easy large-scale production
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Embodiment 1
[0022] Preparation of seed culture solution: inoculate a ring of Bacillus subtilis slant strain cells into the liquid expansion broth nutrient medium, and shake the shaker for 20 hours at 30°C and 180r / min to make the seed culture solution The cell concentration reached 4.1×10 9 cfu / mL; Inoculate a ring of slant strain cells with 1 strain of Bacillus lateralis in the nutrient medium of liquid expansion broth, and culture it on a shaker at 35°C and 200r / min for 24h to make the seed culture medium Bacteria concentration reached 3.5×10 9 cfu / mL; Inoculate a ring of slant strain cells with one strain of Hansenula anomalies in the wort liquid medium, and culture it on a shaker at 28°C and 200r / min for 24 hours to make the bacteria in the seed culture medium The concentration reaches 8.3×10 7 cfu / mL; Inoculate a ring of slant strain cells with one strain of Candida rugosa in the wort liquid medium, and culture it on a shaker at 30°C and 220r / min for 36 hours to make the bacteria i...
Embodiment 2
[0027] Preparation of seed culture solution: Inoculate one ring of Bacillus subtilis slant strain cells into the liquid expansion broth nutrient medium, and culture it on a shaker at 35°C and 200r / min for 18 hours to make the seed culture solution The cell concentration reached 2.3×10 9 cfu / mL; Inoculate a ring of slant strain cells with 1 strain of Bacillus lateralis in the nutrient medium of liquid expansion broth, and culture on a shaker for 30 hours at 37°C and 220r / min to make the seed culture medium The cell concentration reached 6.2×10 9 cfu / mL; Inoculate a ring of slant strain cells with one strain of Hansenula anomalies in the wort liquid medium, and culture it on a shaker for 30 hours at 26°C and 180r / min to make the bacteria in the seed culture medium The concentration reaches 1.8×10 8 cfu / mL; Inoculate a ring of slant strain cells with one strain of Candida rugosa in the wort liquid medium, and culture it on a shaker for 30 hours at 28°C and 180r / min to make the ...
Embodiment 3
[0032] Preparation of seed culture solution: Inoculate one ring of Bacillus subtilis slant strain cells into the liquid expansion broth nutrient medium, and shake the shaker for 24 hours at 37°C and 220r / min to make the seed culture solution The cell concentration reached 6.0×10 9 cfu / mL; Inoculate a ring of slant strain cells with 1 strain of Bacillus lateralis in the nutrient medium of liquid expansion broth, and culture it on a shaker for 36 hours at 36°C and 180r / min to make the seed culture medium The cell concentration reached 8.9×10 9 cfu / mL; Inoculate a ring of slant strain cells with one strain of Hansenula anomalies in the wort liquid medium, and culture it on a shaker at 27°C and 220r / min for 20h to make the bacteria in the seed culture medium The concentration reaches 5.5×10 7 cfu / mL; inoculate a ring of slant strain cells with one strain of Candida rugosa in the wort liquid medium, and culture it on a shaker at 29°C and 200r / min for 24 hours to make the bacteria...
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