Penicillium citrinum LJ318 for degrading chlortetracycline

A technique of chlortetracycline and penicillium citrinum, applied in the field of microorganisms, can solve the problems of few research reports and no research reports, and achieve the effects of convenient use, good degradation, tolerance and wide concentration range of use

Inactive Publication Date: 2013-09-11
BEIJING INSTITUTE OF TECHNOLOGYGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Yet, to the screening of aureomycin-degrading bacterial strain and the research that utilizes high-efficiency bacterial strain to degrade aureomycin, domestic and foreign research reports are few
And the research about Penicillium citrinum degrading aureomycin aspect, there is no research report both at home and abroad

Method used

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  • Penicillium citrinum LJ318 for degrading chlortetracycline
  • Penicillium citrinum LJ318 for degrading chlortetracycline

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Screening method for a strain of Penicillium citrinum LJ318 degrading aureomycin

[0030] 1.1 Material preparation

[0031] Source of bacteria samples: collected from the waste of aureomycin production enterprises.

[0032] Inorganic salt solid medium: (NH4) 2 SO 4 2.00g, K 2 HPO 4 0.50g, KH 2 PO 4 0.50g, MgSO 4 7H2O 0.50g, NaCl 0.20g, CaCl 2 0.1g, FeSO 4 0.01g, 0.015g EDTA, 13.00g agar, 2.00g / L glucose, dissolved in 1000ml distilled water. After sterilizing at 115°C for 20 minutes, add 2.00 g / L aureomycin to the ultra-clean workbench.

[0033] Martin's medium: peptone 6.0g, glucose 10.0g, KH 2 PO 4 1.0g, MgSO 4 ·7H2O 0.5g, dissolved in 1000ml of distilled water. Sterilize at 115°C for 20 minutes.

[0034] Select medium: (NH4) 2 SO 4 2.00,K 2 HPO 4 0.50, KH 2 PO 4 0.50, MgSO 4 7H2O 0.50, NaCl 0.20, CaCl 2 0.10, FeSO 4 0.01g, 0.015g of EDTA, dissolved in 1000ml of distilled water. Sterilize at 115°C for 20 minutes, after cooling, a...

Embodiment 2

[0074] Growth and degradation experiments of strain LJ318 in aureomycin as the only carbon source medium:

[0075] Prepare aureomycin as the only carbon source and the concentrations of aureomycin are 25mg / L, 50mg / L, 100mg / L, 1000mg / L, 2000mg / L, 3000mg / L, 4000mg / L, 5000mg / L, 10000mg / L Inorganic salt solid medium of L. Pick a few LJ318 colonies from the activated culture medium, streak on the above-mentioned inorganic salt solid medium plate, and culture in the dark at 28°C. It is known through observation that the concentration of aureomycin for bacterial strain LJ318 is 25mg / L, 50mg / L , 100mg / L, 1000mg / L, 2000mg / L, 3000mg / L, 4000mg / L, 5000mg / L, and 10000mg / L plates all grew hyphae. It shows that the strain LJ318 can grow in the medium with the concentration of aureomycin being 25mg / L-10000mg / L.

[0076] The activated strain LJ318 was inserted into the inorganic salt liquid medium with chlortetracycline as the sole carbon source and the concentration of chlortetracycline was...

Embodiment 3

[0078] Strain LJ318 treatment experiment on aureomycin residue:

[0079] The strain LJ318 was activated with Martin's medium, then inoculated into fresh chlortetracycline residue, cultured in the dark at 30°C, and samples were taken regularly to determine the residual amount of chlortetracycline in the residue and the degradation rate was calculated.

[0080] The method for measuring the pH value is as follows: take 1 g of fungus residue and add 10 ml of distilled water, stir it evenly, and measure it with an acidity meter.

[0081] The determination method of chlortetracycline is: accurately weigh 2.0000g of bacteria residue, extract with 20mL of acetone: hydrochloric acid solution: water = 13:1:6 extract, centrifuge the extract for 5min at 8000r / min, and take the supernatant , filter with a filter membrane of 22 μm, utilize high performance liquid chromatography to measure the aureomycin content in the filtrate and calculate the degradation rate, the HPLC conditions are the sa...

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PUM

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Abstract

The invention relates to a strain of Penicillium citrinum LJ318 capable of degrading chlortetracycline and belongs to the technical field of microorganisms. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) in March 3rd, 2012, and the preservation number of the strain is CGMCC No.5850. An rDNA-ITS (Ribosomal DNA internal transcribed spacer) gene sequence of the chlortetracycline degrading strain LJ318 consists of 544 basic groups. The strain is wide in chlortetracycline-tolerating range and has relatively good degradation effects on the residual chlortetracycline in both liquid and solid wastes.

Description

technical field [0001] The invention relates to a strain of Penicillium citrinum LJ318 capable of degrading aureomycin, belonging to the technical field of microorganisms. Background technique [0002] Chlortetracycline (CTC), also known as chlortetracycline, is a broad-spectrum antibiotic that can inhibit Gram-positive bacteria, Gram-negative bacteria, spirochetes, rickettsia, and large viruses. It can prevent a variety of animal diseases and is widely used in the prevention and control of livestock and poultry diseases. [0003] As a veterinary drug, aureomycin is mostly excreted in its original form after being metabolized in the animal body, and it is not easy to biodegrade in the environment, forming environmental accumulation and residue. Aureomycin production enterprises will produce a large amount of waste during the fermentation process of aureomycin production. If these wastes are not properly treated and enter the environment, they will also have toxic and inhibi...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N15/31A62D3/02C12R1/80A62D101/28
Inventor 李艳菊姚民僕杨正礼
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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