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Droplet micro-fluidic chip and operation method thereof

A microfluidic chip and droplet technology, applied in chemical instruments and methods, laboratory utensils, chemical/physical/physicochemical processes, etc., can solve problems that are difficult to achieve precise control, expensive, flow field stability and uniformity and other issues, to achieve the effect of mild splitting and fusion conditions

Inactive Publication Date: 2013-09-11
苏州扬清芯片科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned systems require expensive instruments and equipment on the one hand, and on the other hand, the current flow field stability and uniformity of these pump and valve systems are intersected, making it difficult to achieve precise control
And the external electric field or temperature field has a certain destructive effect on the droplet.

Method used

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  • Droplet micro-fluidic chip and operation method thereof
  • Droplet micro-fluidic chip and operation method thereof
  • Droplet micro-fluidic chip and operation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 A microfluidic chip for droplet generation

[0021] A microfluidic chip structure for droplet generation such as figure 1 As shown, the microfluidic chip is made of glass, and the width and depth of channels a, b and c are 200 microns and 80 microns respectively. The structure of the electroosmotic pump d is as follows figure 2 As shown, the electroosmotic pump contains a confluence buffer f and two Y-shaped intersected packed column channels g and h. The two filled column channels are filled with glass microspheres with a particle size of 5 microns, and the surfaces are respectively modified with positive and negative charges. .

[0022] During the experiment, firstly, the internal channel of the chip is filled with 0.1M borax buffer solution, the filled column g of the electroosmotic pump is connected to the negative electrode of the external electric field, and the filled column h is connected to the positive electrode of the external electric field. Und...

Embodiment 2

[0023] Example 2 A microfluidic chip for droplet splitting

[0024] A microfluidic chip structure for droplet splitting such as image 3 As shown, the microfluidic chip is made of polycarbonate, and the chip includes a droplet generation channel A, a droplet splitting channel B and two electroosmotic pumps C and D. Wherein, the droplet splitting channel B is composed of two branch channels, and the electroosmotic pumps C and D are respectively connected to the two branch channels of the splitting channel B. All channels have a width and depth of 200 μm and 80 μm. The structural composition of electroosmotic pumps C and D is as follows figure 2 As shown, the electroosmotic pump contains a confluence buffer f and two Y-shaped intersected packed column channels g and h. The two filled column channels are filled with glass microspheres with a particle size of 5 microns, and the surfaces are respectively modified with positive and negative charges. .

[0025] During the experi...

Embodiment 3

[0026] Example 3 A microfluidic chip for droplet fusion

[0027] A microfluidic chip structure for droplet fusion such as Figure 4 As shown, the microfluidic chip is made of polydimethylsiloxane, and the chip includes two droplet generation channels E and F, a droplet fusion channel G and an electroosmotic pump H. Wherein, the droplet fusion channel G is formed by the intersection of two channels, and the electroosmotic pump H is connected to the intersection channel of the fusion channel. All channels have a width and depth of 200 μm and 80 μm. The structural composition of electroosmotic pumps C and D is as follows figure 2 As shown, the electroosmotic pump contains a confluence buffer f and two Y-shaped intersected packed column channels g and h. The two filled column channels are filled with glass microspheres with a particle size of 5 microns, and the surfaces are respectively modified with positive and negative charges. .

[0028] During the experiment, firstly, th...

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Abstract

The invention provides a novel droplet micro-fluidic chip and an operation method thereof, and in particular relates to a droplet micro-fluidic chip for integrating an electroosmotic pump. The droplet chip consists of a sample pool, a micro-channel and a micro-electroosmotic pump. One or the combination of micro-droplet generation, splitting or fusion is controlled by regulating negative pressure generated by the micro-electroosmotic pump. Droplet operation is realized in a non-electric field environment. Under the condition, on one hand, droplets can be accurately operated by regulating the pressure of the electroosmotic pump; on the other hand, the droplet generation, splitting and fusion conditions are mild, and interference from an additional electric field is avoided.

Description

technical field [0001] The invention relates to the application technology of a microfluidic chip, and in particular provides a microfluidic chip droplet manipulation method based on an electroosmotic pump. Background technique [0002] In the prior art, the microfluidic chip is an emerging technology platform. On a chip of a few square centimeters, the fluid is controlled by a network of micro-channels to complete various operations in conventional chemical or biological laboratories. Micro-droplet (droplet) is a technology developed on microfluidic chips in recent years to manipulate small-volume liquids. Its principle is: two immiscible liquids are used as the continuous phase, The other is the dispersed phase. After the continuous phase and the dispersed phase enter the microchannel at the same time, under the action of the microchannel, the dispersed phase is distributed in the continuous phase in the form of a small volume (10-15L-10-9L) unit, forming a A series of d...

Claims

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Application Information

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IPC IPC(8): B01L3/00B01J19/00
Inventor 叶嘉明
Owner 苏州扬清芯片科技有限公司
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