Dendrobium officinale tissue culture seedling method

Abstract

The invention discloses a dendrobium officinale tissue culture seedling method which comprises the following operating steps of: manufacturing a culture medium of dendrobium officinale tissue culture seedlings, filling the components in a container according to the following weight ratio: 1000-1500g of banana stirred into the paste, 200-300g of white sugar, 90-105ml of ferrous sulfate, 25-33g of ammonium nitrate, 35-40g of potassium nitrate, 5-8g of magnesium sulfate, 8-12ml of glycine, 1-3ml of vitamin B1, 1-3ml of vitamin B2, 20-30ml of sugar amino acid, 20-30ml of naphthylacetic acid and 1000ml of running water, boiling, filling the culture medium into a tissue culture bottle through a filling machine, sterilizing and disinfecting; seeding on the culture medium by using pollen of mature dendrobium officinale seeds, and growing mother seedlings within three months. The cultured dendrobium officinale tissue culture seedlings are healthy and strong and normal in leaf color, 4-5 seedlings are grown, the survival rate is high, and the survival rate can be over 95 percent.

Description

technical field [0001] The invention relates to a method for tissue culture seedlings of dendrobium officinale. Background technique [0002] Dendrobium candidum, also known as black knot grass, belongs to the Dendrobium genus of Orchidaceae, is a perennial epiphytic herb of Orchidaceae, and is a precious wild medicinal plant. Born on the semi-shady rocks in the mountains at an altitude of 1600 meters, it likes a warm and humid climate and a semi-shady and semi-yang environment. It is not cold-resistant, and generally can withstand a low temperature of -5°C. Dendrobium can be divided into dozens of species such as yellow grass, nobile, and horsewhip. Dendrobium candidum and Dendrobium nobile are the best products of dendrobium. Dendrobium candidum is listed as "the first of the nine Chinese immortal grasses". A tribute to the royal family, Dendrobium officinale got its name because of its iron-green skin. Dendrobium candidum has unique medicinal value, and its stem is used...

AI Technical Summary

Problems solved by technology

The tender stems of a few varieties such as Dendrobium candidum are twisted into spiral or spring-shaped and dried in the sun. The commercial product is called Dendrobium earrings. However, due to the slow growth cycle, it is extremely difficult to survive. The wild Dendrobium candidum is on the verge of extinction, and the survival rate of artificial seedlings is low.