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Method for determining contents of cystine, cysteine and salt thereof in amino acid injection

A cysteine ​​and determination method technology, applied in the content determination of cysteine ​​and its salts, in the field of cystine, can solve the problems of affecting analytical sensitivity, interference in content analysis, expensive reagents, etc., and save detection time. , The effect of strong exclusivity and low price

Active Publication Date: 2014-05-14
GUANGDONG LITAI PHARM CO LTD
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Problems solved by technology

[0003] At present, the method for analyzing the contents of cystine, cysteine ​​and their salts in amino acid nutritional injections is generally the waters derivatization method, which has the following disadvantages that are difficult to overcome: 1) This method uses a special AccQ-FluorTM reagent , due to its monopoly position, the reagent price is relatively expensive; 2) In the process of collecting cystine, cysteine ​​and its salts during analysis, other amino acids are also collected at the same time, resulting in many unknown peaks in the analysis process, Other collected substances interfere with the content analysis of cystine, cysteine ​​and their salts; 3) Other collected substances also affect the sensitivity of the analysis

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  • Method for determining contents of cystine, cysteine and salt thereof in amino acid injection
  • Method for determining contents of cystine, cysteine and salt thereof in amino acid injection
  • Method for determining contents of cystine, cysteine and salt thereof in amino acid injection

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Embodiment Construction

[0029] The present invention will be further described below in conjunction with specific examples.

[0030] Determination of content of cysteine ​​hydrochloride and its salts, cystine and its salts in amino acid injection

[0031] 1. Prepare the required solution:

[0032] Preparation of special mobile phase A6 solution: Take 12.0g of trisodium citrate dihydrate, 7.6g of sodium chloride, 6.0g of citric acid, 1.0g of boric acid, 120ml of methanol, 7.78ml of 37% hydrochloric acid, and 0.1ml of caprylic acid, and place them in a 1000ml measuring bottle. Add water to the volume and adjust the pH to 2.7;

[0033] Prepare a special sodium hydroxide regeneration solution: take 18.0g of sodium hydroxide and put it in a 1000ml measuring bottle, add water to make up to the mark;

[0034] Prepare potassium-sodium buffer solution (pH5.51): Take 272.0g of sodium acetate trihydrate and 196.0g of potassium acetate, add them to 400ml of water, stir while adding, add 200ml of acetic acid at...

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Abstract

The invention relates to a method for determining contents of cystine, cysteine and salt thereof in amino acid injection. The method comprises the following steps: measuring a sample to be detected and a reference solution precisely, adding 0.1-1% of sodium pyrosulfite solution, shaking up, heating by 70-100 DEG C. for 10-60 minutes, fetching out, adding formic acid 1.5 ml and 30% superoxol 1.0 ml, shaking up, placing in 30+-2 DEG C. for 30 minutes, adding water to the scale, shaking up, fetching the sample to be tested and injecting into a chromatograph with cation exchange resin, carrying out gradient elution and regeneration balance by a special-purpose mobile phase A6 solution and a special-purpose sodium hydroxide regeneration liquid, carrying out a post-column derivatization reaction by a special-purpose derivatization reagent ninhydrin solution, recording a chromatogram with the detection wavelength 570 nm, and obtaining the contents by calculating peak areas according to external standard method. The invention has the beneficial effects that (1) short analysis time and saved detection time; (2) high sensitivity; (3) low price and saved detection cost; (4) good specialization.

Description

technical field [0001] The invention relates to a method for determining the content of cystine, cysteine ​​and salts thereof, in particular to a method for determining the content of cystine, cysteine ​​and salts thereof in amino acid injection. Background technique [0002] Cystine is a non-essential amino acid in the human body, and its increased concentration in the body is considered to be the initiating factor of atherosclerosis. Among them, homocysteine ​​is associated with cardiovascular diseases (coronary artery disease, hypertension), nerve It is closely related to the occurrence of systemic diseases (stroke), diabetes, kidney disease, pregnancy-related diseases, and Alzheimer's disease. In the late 1960s, some scholars noticed that most patients with hereditary homocystinuria had subclinical cardiovascular disease. In 1976, Wichen et al. proposed through epidemiological investigation that homocysteine ​​is an independent risk factor for cardiovascular patients, b...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 严正华方建华罗庆发
Owner GUANGDONG LITAI PHARM CO LTD
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