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Alkaline phosphatase detection reagent

A technology for detecting reagents and phosphatase, which is applied in the field of medical inspection, can solve the problems of lack of inspection professionals and insufficient facilities for automatic biochemical inspection equipment, and achieve good sensitivity, low price, and easy operation

Active Publication Date: 2013-09-04
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] With the "Eleventh Five-Year" plan for the purchase and strengthening of basic equipment for tertiary and secondary hospitals, hospitals above the middle level have good hardware and software facilities, but grassroots medical institutions, especially health centers, clinics and other units are not fully automatic. Biochemical testing equipment and facilities are generally insufficient, and there is not a sufficient number of testing professionals. Therefore, the establishment of a POCT analysis system that is easy to operate, low in price, and real-time reporting is of great significance to the role of grassroots hospitals in disease prevention and diagnosis and treatment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Diluent:

[0042] Tris hydroxymethyl amino buffer (pH7.0) 0.1mol / L

[0043] TWEEN80 5%

[0044] Proclin300 0.1%

[0045] Bilirubin oxidase 1KU / L

[0046] Vitamin C oxidase 1KU / L

[0047] Reagent:

[0048] 2-Amino-2-methyl-propanol (pH10.0) 0.1mol / L

[0049] Magnesium acetate 5mmol / L

[0050] Zinc sulfate 5mmol / L

[0051] Hydroxyethylethylenediaminetriacetic acid 5mmol / L

[0052] Disodium p-nitrophenyl phosphate 20mmol / L

[0053] Sodium Lauryl Sulfate 10g / L

[0054] Potassium sorbate 0.1g / L

[0055] Bovine serum albumin 5g / L

Embodiment 2

[0057] Diluent:

[0058] Tris hydroxymethyl amino buffer (pH7.5) 0.5mol / L

[0059] TRITONX-100 5%

[0060] Methylparaben 1%

[0061] Potassium ferrocyanide 1mol / L

[0062] Vitamin C oxidase 2KU / L

[0063] Reagent:

[0064] 2-amino-2-methyl-propanol (pH10.5) 0.2mol / L

[0065] Magnesium acetate 10mmol / L

[0066] Zinc sulfate 10mmol / L

[0067] Hydroxyethylethylenediaminetriacetic acid 20mmol / L

[0068] Disodium p-nitrophenyl phosphate 40mmol / L

[0069] Sodium Lauryl Sulfate 20g / L

[0070] Proclin300 1g / L

[0071] Trehalose 10g / L

Embodiment 3

[0073] Diluent:

[0074] 3-Morpholinepropanesulfonic acid buffer (pH6.5) 1.0mol / L

[0075] SPAN20 10%

[0076] Sodium Benzoate 1%

[0077] Potassium ferricyanide 10mol / L

[0078] Vitamin C oxidase 10KU / L

[0079] Reagent:

[0080] 2-amino-2-methyl-propanol (11.0) 1.0mol / L

[0081] Magnesium acetate 100mmol / L

[0082] Zinc sulfate 100mmol / L

[0083] Hydroxyethylethylenediaminetriacetic acid 80mmol / L

[0084] Disodium p-nitrophenyl phosphate 50mmol / L

[0085] Sodium Lauryl Sulfate 30g / L

[0086] Proclin300 1g / L

[0087] Fatty alcohol polyoxyethylene ether 10g / L

[0088] The performance of the reagent obtained in Example 1 of the present invention is described below in conjunction with the table.

[0089] 1. Precision

[0090] Table 1. Precision evaluation results

[0091]

[0092] 2. Linear

[0093] The high-concentration serum samples collected from the hospital were diluted into different concentrations of analytes for detection, and the ALP linearity results...

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Abstract

The invention discloses an alkaline phosphatase detection reagent which comprises a diluent and a reaction reagent, wherein the diluent is composed of buffer, surfactant, preservative, anti-bilirubin interference agent and vitamin C oxidase; and the reaction reagent is composed of 2-amino-2-methyl-propanol, magnesium acetate, zinc sulfate, hydroxyethyl ethylenediamine triacetic acid, p-nitrophenyl disodium phosphate, sodium lauryl sulfate, preservative and freeze-drying protective agent. The detection reagent disclosed by the invention has favorable sensitivity, accuracy, precision and linearity, and can completely satisfy the clinical examination requirements.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to an alkaline phosphatase detection reagent used in a POCT analyzer. Background technique [0002] Liver disease is a common and extremely harmful disease, and its timely diagnosis and treatment play an important role in the prevention, diagnosis and treatment of the disease. Alkaline phosphatase (ALP) is widely distributed in various organs of the human body, among which Liver is the most. Clinically, ALP is mainly used for the examination of obstructive jaundice, primary liver cancer, secondary liver cancer, and cholestatic hepatitis. Due to the obstruction of bile excretion in the intrahepatic biliary tract, the reflux of blood caused a significant increase in serum ALP. At present, ALP is generally detected by various large-scale automatic biochemical analyzers. However, due to the high price of large-scale automatic biochemical analyzers and complicated operations, o...

Claims

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Application Information

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IPC IPC(8): C12Q1/42
Inventor 邹炳德邹继华沃燕波张桂春周海滨
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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