Method for extracting polysaccharide from litsea roots

A technology of wood ginger seeds and polysaccharides is applied in the field of extracting polysaccharides to achieve the effects of increasing yield, shortening extraction time and low energy consumption

Inactive Publication Date: 2013-09-04
NANJING ZELANG AGRI DEV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no domestic patent related to the industrial extraction method of polysaccharides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Crush the roots of Mujiang seeds, take 1kg, infiltrate with water, and extract by ultra-high pressure method. The particle size is controlled to 60 meshes, the solid-liquid volume ratio is 1:10, the pressure is 400MPa, and the pressure is maintained for 5min. Repeat 2 times. Add protease to 10 activity units / L at ℃, enzymolyse for 4 hours, filter the enzymatic hydrolysate by ultrafiltration with 6000 hollow cellulose membrane, collect the permeate and concentrate it under 85℃ in vacuum, and concentrate to 1 / 3 of the original volume The concentrated solution was added with 95% ethanol, stirred, allowed to stand for precipitation, separated by a centrifuge, the centrifuge speed was 3000r / min, and the precipitate was obtained, which was placed in a vacuum drying box for vacuum drying at a drying temperature of 75°C to obtain the ginger root The polysaccharide is 55g, the content is 71%.

Embodiment 2

[0019] Crush the root of Mujiang seed, take 1kg, infiltrate with water, and extract by ultra-high pressure method. The particle size is controlled to 60 mesh, the solid-to-liquid volume ratio is 1:18, the pressure is 400MPa, the pressure is maintained for 3min, repeat 3 times, and the combined extract is at 50 Add protease to 20 activity units / L at ℃, enzymatic hydrolysis for 4 hours, filter the enzymatic hydrolysate by ultrafiltration with a 10,000 hollow cellulose membrane, collect the permeate and concentrate it under 85℃ in vacuum, and concentrate to 1 / 3 of the original volume The concentrated solution was added with 95% ethanol, stirred, allowed to stand for precipitation, separated by a centrifuge, the centrifuge speed was 3000r / min, and the precipitate was obtained, which was placed in a vacuum drying box for vacuum drying at a drying temperature of 75°C to obtain the ginger root The polysaccharide is 49g, the content is 73%.

Embodiment 3

[0021] Crush the roots of Mujiang seeds, take 1kg, infiltrate with water, and extract by ultra-high pressure method. The particle size is controlled to 60 mesh, the solid-to-liquid volume ratio is 1:15, the pressure is 400MPa, and the pressure is maintained for 4min. Repeat 3 times, and combine the extracts at 50 Add protease to 20 activity units / L at ℃, enzymatic hydrolysis for 4 hours, filter the enzymatic hydrolysate by ultrafiltration with a 10,000 hollow cellulose membrane, collect the permeate and concentrate it under 85℃ in vacuum, and concentrate to 1 / 3 of the original volume The concentrated solution was added with 95% ethanol, stirred, allowed to stand for precipitation, separated by a centrifuge, the centrifuge speed was 3000r / min, and the precipitate was obtained, which was placed in a vacuum drying box for vacuum drying at a drying temperature of 75°C to obtain the ginger root Polysaccharide is 43g, content is 70%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for extracting polysaccharide from litsea roots, belonging to the field of biotechnologies. The method comprises the steps of crushing litsea roots, adding water to infiltrate the crushed litsea roots, extracting by adopting an ultrahigh pressure method, controlling the particle size to be 60 meshes, the solid-liquid volume ratio to be (1: 10) to (1: 18) and the pressure to be 400 MPa, maintaining pressure for 3-5 min, repeating 2-3 times, adding protease to extract so as to carry out enzymolysis, filtering enzymatic hydrolysate, carrying out ultrafiltration on the filtered enzymatic hydrolysate by using an ultrafiltration membrane, collecting permeate, concentrating the permeate by using an ultrafiltration membrane, collecting concentrate, carrying out ethanol precipitation on the concentrate, and then, carrying out vacuum drying, thereby obtaining litsea root polysaccharide. According to the method disclosed by the invention, the operation is simple, the extraction ratio is high, the energy consumption is low, no pollution is caused, and the industrial production is facilitated.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting polysaccharides from the root of cayenne japonica. Background technique [0002] Mujiangzi, also known as Muxiangzi, is mainly distributed in the provinces and regions south of the Yangtze River in my country until Tibet. Nature and flavor are pungent, slightly bitter, fragrant, non-toxic. It blooms from November to April of the next year, and the branches and leaves are fragrant. The root bark and leaves are used medicinally. Mujiangzi has the effects of warming the kidney and invigorating the stomach, promoting qi and dispelling stagnation, and is used to treat stomach pain, vomiting, and unknown swelling and toxin embolism. [0003] Studies in recent years have shown that plant polysaccharides not only have unique effects in enhancing the body's immunity, anti-tumor, anti-virus, anti-oxidation, anti-aging, lowering blood sugar, lowering blood...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08B37/00
Inventor 苏刘花万冬梅
Owner NANJING ZELANG AGRI DEV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap