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Novel freeze-drying platelet-rich fibrin, as well as preparation and application thereof

A platelet-rich, fibrin-rich technology, applied in the fields of medical science, absorbent pads, prostheses, etc., can solve problems such as undiscovered research reports, and achieve the effects of reducing immunogenicity, no immune rejection, and simple preparation method.

Inactive Publication Date: 2013-09-04
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, through consulting domestic and foreign literature, no research reports in this area have been found.

Method used

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  • Novel freeze-drying platelet-rich fibrin, as well as preparation and application thereof
  • Novel freeze-drying platelet-rich fibrin, as well as preparation and application thereof
  • Novel freeze-drying platelet-rich fibrin, as well as preparation and application thereof

Examples

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Embodiment Construction

[0035] The implementation of the present invention will be described in detail below in conjunction with the drawings and examples.

[0036] The preparation method of the platelet-rich fibrin (PRF) biological material by the freeze-drying method of the present invention comprises the following steps:

[0037] The first step is to draw fresh blood from humans or animals with a sterile syringe (draw a certain volume of non-anticoagulated blood according to the needs and individual conditions), quickly add it to a sterilized centrifuge tube, immediately centrifuge at 3000r / min for 10 minutes, and place it at 4°C Let stand in the refrigerator for 30 minutes.

[0038] In the second step, after the blood is coagulated, take out the blood coagulation, remove the blood clot at the bottom, and keep the light yellow gel-like substance, that is, platelet-rich fibrin, wrap it with sterile gauze and squeeze it, and remove the liquid in it. Extrude to form a film-like object, put it into a...

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Abstract

The invention discloses a preparation method of a novel freeze-drying platelet-rich fibrin. The method comprises steps of: centrifuging the fresh blood to obtain the gel-like platelet-rich fibrin (PRF), freeze drying the gel-like platelet-rich fibrin for 24 hours in the vacuum low-temperature environment of 400 Pa and minus 45-minus 4 DEG C so as to obtain the freeze-drying platelet-rich fibrin (PRF) with bioactivity, and preparing the sheet, granular or powdery freeze-drying platelet-rich fibrin (PRF) according requirements so as to be used for preparing trauma auxiliary materials, tissue engineering scaffolds or other biological materials for recombination use. The method can maintain the activity of the fresh PRF, so that the PRF can be easily and refrigerated for a long time, is convenient and flexible to use, can be used as the trauma auxiliary material or the scaffold material to be compounded with other tissue engineering materials and can be applied to war injury repair and tissue defect repair.

Description

technical field [0001] The invention belongs to the field of biological materials and tissue engineering, and relates to the preparation technology of biological materials, in particular to a novel freeze-dried platelet-rich fibrin and its preparation and application. Background technique [0002] French scientist Choukroun proposed a new generation of platelet-rich concentrate in 2000, that is, platelet-rich fibrin (PRF), because of its simple production method, low cost, rich in growth factors, and long acting time, certain The scaffolding effect, as well as certain anti-infection and immune functions and many other advantages, have received extensive attention in the research of trauma and defect repair of various soft and hard tissues, and have achieved satisfactory results. However, due to the limitations of PRF preparation methods, its application is limited to individualized on-the-go, that is, after fresh blood is collected, it must be centrifuged rapidly within a fe...

Claims

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Application Information

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IPC IPC(8): A61L15/32A61L15/44A61L27/54A61L27/22
Inventor 刘斌杨勇邢红艳杨涛徐海燕安然
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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