A method for constructing an ion channel on a liquid/liquid interface and a method for detecting the influence of the size of the ion channel on ion transfer

Abstract

The invention discloses a method for building a K+ ion channel on liquid / liquid interfaces. The method for building the K+ ion channel on the liquid / liquid interfaces comprises the following steps that (1) an alumina template is prepared; (2) the prepared alumina template is fixed on a polished smooth plastic pipe through a conductive adhesive, a three-bifurcation pipe is used as an electrolytic tank, organic phases are first added, then a potassium chloride solution is added in the plastic pipe with the fixed alumina template, the plastic pipe is placed on a three-bifurcation main pipe, the alumina template is right located on the interfaces where two solutions are not dissolved with each other, and therefore the ion channel of the liquid / liquid interfaces is formed. The method can be used for simulating a biological membrane and researching the dynamic process that ions pass through the ion channel, has important significance in researching ion transferring, knowing, understanding and mastering a large number of important physiological processes and revealing metabolic processes of substances and energy in biological bodies, and builds a platform for simulating the ions in a biological system.

Description

technical field [0001] The invention relates to a method for constructing an ion channel at two immiscible interfaces and a detection method for the influence of the size of the ion channel on ion transfer. Background technique [0002] In the research of life sciences, the study of ion channels on the cell membrane occupies a very important position. It is a kind of membrane receptor, its molecular structure is similar, it has a specific drug binding site, and it plays an important role in intracellular and intercellular signal transmission. Ion channels were first proposed by Hodgkin and Huxley in 1952. Subsequently, a lot of research focused on the structure and function of ion channels. In 2003, Rod Mackinnon pioneered the discovery of the structure of potassium ion channels. With the rapid development of biophysics and molecular biology, new research techniques, including patch clamp technology, molecular cloning and gene mutation technology, have been widely used, and...

AI Technical Summary

Problems solved by technology

[0004] The above-mentioned method of constructing ion channels from the perspective of biotechnology is very complicated in terms of equipment, and the operation is also very cumbersome. The research on the molecular structure and function of ion channels cannot be further developed.

Images