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Leucine aminopeptidase detection reagent

A technology of leucine aminopeptidase and detection reagent, which is applied in the field of medical inspection, can solve the problems of lack of inspection professionals, insufficient automatic biochemical inspection equipment and facilities, and achieves the effects of good sensitivity, low price and easy operation

Active Publication Date: 2013-08-28
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] With the "Eleventh Five-Year" plan for the purchase and strengthening of basic equipment for tertiary and secondary hospitals, hospitals above the middle level have good hardware and software facilities, but grassroots medical institutions, especially health centers, clinics and other units are not fully automatic. Biochemical testing equipment and facilities are generally insufficient, and there is not a sufficient number of testing professionals. Therefore, the establishment of a POCT analysis system that is easy to operate, low in price, and real-time reporting is of great significance to the role of grassroots hospitals in disease prevention and diagnosis and treatment.

Method used

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  • Leucine aminopeptidase detection reagent
  • Leucine aminopeptidase detection reagent
  • Leucine aminopeptidase detection reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Diluent:

[0038] Tris hydroxymethyl amino buffer (pH7.0) 0.1mol / L

[0039] TWEEN80 5%

[0040] Proclin300 0.1%

[0041] Bilirubin oxidase 1KU / L

[0042] Vitamin C oxidase 1KU / L

[0043] Reagent:

[0044] Tris hydroxymethyl amino buffer (pH6.0) 0.1mol / L

[0045] Sodium chloride 1mmol / L

[0046] L-leucine-4-nitroaniline 20mmol / L

[0047] Potassium sorbate 0.1g / L

[0048] Bovine serum albumin 5g / L

Embodiment 2

[0050] Diluent:

[0051] Tris hydroxymethyl amino buffer (pH7.5) 0.5mol / L

[0052] TRITON X-100 5%

[0053] Methylparaben 1%

[0054] Potassium ferrocyanide 1mol / L

[0055] Vitamin C oxidase 2KU / L

[0056] Reagent:

[0057] 3-(N-morpholine)sodium ethanesulfonate buffer solution (pH7.2) 0.5mol / L

[0058] Sodium chloride 5mmol / L

[0059] L-leucine-4-nitroaniline 50mmol / L

[0060] Proclin300 1g / L

[0061] Trehalose 10g / L

Embodiment 3

[0063] Diluent:

[0064] 3-Morpholinepropanesulfonic acid buffer (pH6.5) 1.0mol / L

[0065] SPAN20 10%

[0066] Sodium Benzoate 1%

[0067] Potassium ferricyanide 10mol / L

[0068] Vitamin C oxidase 10KU / L

[0069] Reagent:

[0070] Piperazine-N,N-bis(2-hydroxyethanesulfonic acid) buffer solution (pH8.0) 1mol / L

[0071] Sodium chloride 20mmol / L

[0072] L-leucine-4-nitroaniline 100mmol / L

[0073] Proclin300 1g / L

[0074] Fatty alcohol polyoxyethylene ether 10g / L

[0075] The performance of the reagent obtained in Example 1 of the present invention is described below in conjunction with the table.

[0076] 1. Precision

[0077] Table 1. Precision evaluation results

[0078]

[0079] 2. Linear

[0080] The standard serum sample is added with different concentrations of analytes for detection, and the LAP linearity results are shown in figure 1 .

[0081] 3. Methodological comparison test

[0082] Compared with the LAP results measured on the automatic biochemical...

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Abstract

The invention discloses a leucine aminopeptidase detection reagent which is characterized by comprising a diluent and a reaction reagent, wherein the diluent is composed of a buffer solution, a surfactant, a preservative, a bilirubin interference remover and a vitamin C oxidase; and the reaction reagent is composed of a buffer solution, sodium chloride, L-leucine-4-nitroaniline, 0.1-1 g / L preservative and 10-30 g / L freeze-drying protective agent. The detection reagent disclosed by the invention has favorable sensitivity, accuracy, precision and linearity, and can completely satisfy the clinical examination requirements.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a leucine aminopeptidase detection reagent for a POCT analyzer. Background technique [0002] Liver disease is a common and extremely harmful disease. Its timely diagnosis and treatment play an important role in the prevention, diagnosis and treatment of the disease. Leucine aminopeptidase (LAP, EC3.4.11.1) is widely distributed In tissues such as liver, pancreas, and kidney, when human liver, gallbladder, pancreas, and other tissues have lesions, serum LAP levels can increase. Determination of serum LAP can be used as another indicator for the diagnosis of liver diseases, and it is valuable for the differential diagnosis of cholestatic hepatitis, hepatic obstruction and pancreatic cancer. The positive rate of detection of liver cancer is higher than that of ADA, and it is more sensitive than GGT to reflect liver diseases. The detection of LAP can also reflect the natur...

Claims

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Application Information

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IPC IPC(8): C12Q1/37C12Q1/26
Inventor 邹炳德邹继华沃燕波张桂春周海滨
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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