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Application of pleuromutilin for increasing yield of daptomycin

A technology of pleuromutilin and daptomycin, which is applied in the field of microbial fermentation, can solve the problems of large strain screening workload, affecting the efficiency of mutation breeding, non-directional mutation, etc., and achieves small screening workload and high efficiency. , the effect of broadening the application field

Inactive Publication Date: 2013-08-14
SOUTHWEST UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

However, because the mutations generated by the traditional mutagenesis method are random and non-directional, the screening workload of the current strains is large, which directly affects the work efficiency of mutation breeding.

Method used

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  • Application of pleuromutilin for increasing yield of daptomycin
  • Application of pleuromutilin for increasing yield of daptomycin
  • Application of pleuromutilin for increasing yield of daptomycin

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Embodiment Construction

[0014] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental methods for which specific conditions are not indicated in the preferred embodiments are usually in accordance with conventional conditions or in accordance with the conditions suggested by the manufacturer.

[0015] 1. Materials and methods

[0016] The bacterial strain used in the present invention is Streptomyces roseosporus NRRL 15998 (Streptomyces roseosporus NRRL 15998), purchased from the American Standard Biological Collection (ATCC).

[0017] Medium for cultivating Streptomyces roseospora NRRL 15998: AS-1 medium for slant culture, Trypticase Soy Broth Medium (TSB medium) for seed culture, and F10A medium for fermentation culture , the bioactivity test adopts LB medium, and the composition and preparation method of each medium are as follows:

[0018] AS-1 medium (g / L): Accurately weigh 0.2g of L-Ala, 0.2g of L-A...

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Abstract

The invention discloses the application of pleuromutilin for increasing the yield of daptomycin. The application method comprises the following steps: screening anti-pleuromutilin streptomyces roseosporus by using pleuromutilin with an antiseptic concentration being higher than the lowest antiseptic concentration, further screening strains with increased daptomycin yield from the screened resistant strains, and obtaining high-yield strains through a stability test. When daptomycin is produced by using the resistant strains obtained by using the method disclosed by the invention, the yield is increased by about 30%, thereby laying a foundation for the industrial production of daptomycin.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and particularly relates to the application of pleuromutilin in improving the yield of daptomycin. Background technique [0002] Daptomycin is a new type of cyclolipopeptide antibiotic derived from the fermentation of Streptomyces roseosporus. It is the world's first cyclolipopeptide drug developed by Cubist Pharmaceuticals of the United States. Daptomycin consists of a decanoyl side chain attached to the N-terminal tryptophan of a cyclic 13-amino acid peptide, the peptide ring is composed of amino acids and non-proteinogenic amino acids (eg: Asn, OmAsp, 3mGlu, etc.) according to the amide bond or ester bond to form a peptide ring or a carboxylic acid peptide ring, which is a typical cyclolipopeptide compound. Daptomycin has a good broad-spectrum activity against Gram-positive bacteria, and it has inhibitory effects on 35 species of Gram-positive bacteria belonging to 15 genera, especially ...

Claims

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Application Information

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IPC IPC(8): C12P21/04C12R1/465
Inventor 廖国建黎林丽胡昌华
Owner SOUTHWEST UNIVERSITY
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