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Bacillus licheniformis H2 and application thereof

A technology of Bacillus licheniformis and strains, applied in the application, bacteria, antibacterial drugs and other directions, can solve the problems of incurable, incurable, incurable diseases, etc., and achieves recovery of intestinal function, good economic benefits, and adjustment of intestinal bacteria. The effect of group dissonance

Inactive Publication Date: 2013-08-14
SICHUAN AGRI UNIV
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  • Abstract
  • Description
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Problems solved by technology

[0005] Aiming at the current situation that the abuse and prohibition of antibiotics lead to the situation that the disease is difficult to cure and the poultry infected with Clostridium perfringens and necrotic enteritis is difficult to cure or cannot be treated. Bacillus licheniformis H resistant to bile salts, good antibacterial effect and good antagonistic effect on Clostridium perfringens 2 ; also provide the Bacillus licheniformis H 2 The application in veterinary medicine or feed can be used to treat chicken necrotic enteritis caused by Clostridium perfringens and improve the diversity of chicken intestinal microorganisms

Method used

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  • Bacillus licheniformis H2 and application thereof
  • Bacillus licheniformis H2 and application thereof
  • Bacillus licheniformis H2 and application thereof

Examples

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Embodiment 1

[0026] Example 1: The acquisition and identification of "Bacillus licheniformis CCTCC2011133" of the present invention, see figure 1

[0027] 1. Isolation and acquisition of "Bacillus licheniformis CCTCC2011133" of the present invention

[0028] 1.1 Isolation of strains

[0029]The ileum content of healthy chickens raised in the animal room of Sichuan Agricultural University located in Ya'an City, Sichuan Province, China was collected and sent to the laboratory for testing immediately. Take 0.5g of fresh ileal content, put it into a conical flask with 49.5ml of normal saline and glass beads, shake it on a shaker at 37°C for 20min, and then bathe it in a constant temperature water bath at 60°C for 20min. Take 0.5ml of diluted feces and make a ten-fold decremental dilution to 10 -5 . from 10 -3 ~10 -5 Take 100 μL of each dilution and spread it on ordinary nutrient agar medium, and incubate at a constant temperature of 37°C for 12h (see figure 2 ).

[0030] Microscopical...

Embodiment 2

[0050] Embodiment 2: Application of "Bacillus licheniformis CCTCC2011133" of the present invention

[0051] 1. Animal prevention test

[0052] (1) Preventive test design

[0053] Bacillus licheniformis H of the present invention 2 (i.e. "Bacillus licheniformis CCTCC2011133") prepares bacterial agent I according to the routine operation method of agricultural microorganisms, and the number of viable bacteria is 1.0×10 9 cfu / g, feeding healthy one-day-old iron-footed chickens, see Table 3.

[0054] Table 3 Prevention trial design

[0055]

[0056]

[0057] (2) Determination of growth performance

[0058] According to the (Chen Peng. 2011. China Poultry, 33 (11): 11-14) method, the effect on the growth performance of the infected chicken model in the prevention group was determined, see Table 4. The final weight and average daily gain of the blank group and the chicken model added with different doses of "Bacillus licheniformis CCTCC2011133" to prevent infection were h...

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Abstract

The invention discloses Bacillus licheniformis H2 which is resistant to acid, heat and cholate and has good resistance to Clostridium perfringens and application of Bacillus licheniformis H2 in prevention and control of necrotic enteritis in chicken. The strain is obtained by separation, screening and identification, and has the preservation number of CCTCC NO: M2011133; and Bacillus licheniformis H2 can be prepared into a microorganism preparation, and can effectively prevent and control necrotic enteritis in chicken caused by Clostridium perfringens, promote growth of normal physiological anaerobic bacteria in intestinal tract of chicken, improve intestinal tract microorganism diversity and immune organ index of chicken and improve growth performance of chicken when used as a veterinary medicine and chicken feed additive. Bacillus licheniformis H2 is suitable for feed factories, veterinary drug factories and scaled chicken farms.

Description

technical field [0001] The present invention relates to an isolated bacillus licheniformis (Bacillus licheniformis) H which is acid-resistant, heat-resistant and bile salt-resistant and has good resistance to Clostridium perfringens 2 The invention and its application in preventing and treating chicken necrotic enteritis belong to the technical field of preventive veterinary medicine. Background technique [0002] my country is a large poultry-raising country. Due to the rapid development of large-scale and intensive aquaculture, diseases caused by drug residues and pathogenic bacteria pollution in livestock and poultry emerge in an endless stream, which not only endangers the health of animals, but also causes huge economic losses to my country's animal husbandry. Therefore, the prevention and treatment of various diseases of livestock and poultry has become the primary task of the breeding industry. [0003] In recent years, chicken necrotic enteritis caused by Clostridiu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16A23K1/18A61K35/74A61P31/04C12R1/10A61K35/742
Inventor 倪学勤曾东李洁萍曾燕舒刚景波彭焕伟
Owner SICHUAN AGRI UNIV
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